Estimation of Biomechanical Stimulus in Bone Scaffolds in Vivo: Multi-Scale Finite Element Model

2010 ◽  
Author(s):  
Alireza Roshan-Ghias ◽  
Alexandre Terrier ◽  
Dominique P. Pioletti
Keyword(s):  
Tissue Engineering ◽  
Finite Element ◽  
Bone Formation ◽  
Element Model ◽  
Bone Scaffold ◽  
Synthetic Bone ◽  
Multi Scale ◽  
Bone Scaffolds ◽  
Complex Process

Bone formation inside a scaffold is a complex process involving different phenomena, one of which being the mechanical stimulation (1,2). Our goal in bone tissue engineering is, through mechanical considerations, confer osteoinductivity to a synthetic bone scaffold.

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

scholarly journals A Multi-Scale Finite Element Model of Bruising in Soft Connective Tissues

2012 ◽  
Vol 3 ◽  
pp. 1-5 ◽  
Author(s):  
Lu Huang ◽  
Noah Bakker ◽  
James Kim ◽  
Jez Marston ◽  
Ian Grosse ◽  
...  
Keyword(s):  
Finite Element ◽  
Finite Element Model ◽  
Element Model ◽  
Connective Tissues ◽  
Multi Scale

Biomechanical Modeling of Hip Joint Prosthesis

2005 ◽  
Author(s):  
M. C. Gaspar ◽  
A. Mateus ◽  
C. Pereira ◽  
F. V. Antunes
Keyword(s):  
Finite Element ◽  
Hip Joint ◽  
Laser Scanning ◽  
Hip Prosthesis ◽  
Element Model ◽  
Geometrical Model ◽  
Elastic Behavior ◽  
Joint Prosthesis ◽  
Element Analysis

In this work a Bombelli cementless isoelastic RM total hip prosthesis was considered. It was implanted over a course of 14 years on the patient and studied subsequently to its chirurgical replacement. Computed Tomography, radiographies and 3-D laser scanning were used to assess the prosthesis geometry, while the original femur anatomy was modeled based on 2-D radiographies taken at different stages of the in-vivo implant of the prosthesis. A finite element model was developed, based on the generated 3-D geometrical model, considering a linear elastic behavior and typical loading conditions. This analysis allowed determining stress and strain fields throughout bone-prosthesis contact surface and critical areas in terms of micromovements. The developed procedure, consisting of 3-D scanning, generation of geometrical 3-D models and finite element analysis, results in a powerful tool to follow-up and predict failure mechanisms in hip joint prosthesis.

Personalized finite element model of the knee joint in vivo

2006 ◽  
Vol 39 ◽  
pp. S501
Author(s):  
M. Sangeux ◽  
F. Marin ◽  
F. Charleux ◽  
L. Dürselen ◽  
M.-C. Ho Ba Thoa
Keyword(s):  
Finite Element ◽  
Knee Joint ◽  
Finite Element Model ◽  
Element Model
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