Spatiotemporal Deformation of Engineered Tissue During Freezing

2009 ◽  
Author(s):  
Ka Yaw Teo ◽  
J. Craig Dutton ◽  
Bumsoo Han
Keyword(s):  
Extracellular Matrix ◽  
Cellular Viability ◽  
Long Term Storage ◽  
Preservation Technology ◽  
Engineered Tissue ◽  
Frozen State ◽  
Microstructural Integrity ◽  
Long Term Preservation ◽  
Term Storage

A reliable long-term preservation technology is highly desired to provide “off-the-shelf” availability of various engineered tissues (ETs). Among the existing preservation techniques, cryopreservation, which is preserving biomaterials in the frozen state, remains the primary candidate for long-term storage of ETs. One of the most significant challenges in cryopreservation is the lack of consistency in maintaining the functionality of ETs [1]. Since the functionality is closely related to the microstructural integrity of extracellular matrix (ECM) and cellular viability, the ECM microstructure should be maintained as well as the viability of cells [2, 3].

Role of Cells in Freezing-Induced Cell-Fluid Matrix Interactions Within Engineered Tissues

2012 ◽  
Author(s):  
Angela Seawright ◽  
Altug Ozcelikkale ◽  
J. Craig Dutton ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Biological Tissues ◽  
Cellular Viability ◽  
Long Term Storage ◽  
Matrix Interactions ◽  
Term Storage

Cryopreservation can provide long-term storage of various biological tissues, which has significant impact on tissue engineering and regenerative medicine. For successful cryopreservation of tissues, tissue functionality must be maintained including physical properties such as mechanical, optical, and transport properties, as well as cellular viability. Such properties are associated with the extracellular matrix (ECM) microstructure. Thus, the preservation of the ECM microstructure may lead to successful cryopreservation [1,2]. Yet, there is still very little known about changes in the ECM microstructure during freezing/thawing.

scholarly journals EFFECT OF FREEZING ON THE MILK QUALITY OF COWS

2020 ◽  
pp. 54-57
Author(s):  
Lyubov Valentinovna Efimova ◽  
Tatyana Vyacheslavovna Zaznobina ◽  
Elena Vladimirovna Gatilova ◽  
Olga Valeryevna Ivanova
Keyword(s):  
Quality Indicators ◽  
Age Groups ◽  
Milk Quality ◽  
Long Term Storage ◽  
Before And After ◽  
Frozen State ◽  
Term Storage ◽  
Two Age Groups

Due to the fact that milk has a short shelf life, the issue of its freezing and long-term storage in the frozen state is relevant. A study was conducted on the effect of freezing on the milk quality after its defrosting of cows-mothers and cows-daughters of Red-Motley breed. As a result, it was established how milk quality indicators to change after five months of storage in a frozen state in cows of two age groups. A high correlation was established between quality indicators before and after freezing. The influence of the “freezing” factor on milk quality indicators was recognized statistically significant, the “generation” factor – not significant.

Gelatin/PVA composited photochromic film for light printing with fast rewritability and long-term storage ability

2019 ◽  
Vol 7 (40) ◽  
pp. 12518-12522 ◽  
Author(s):  
Yongqi Yang ◽  
Jianyu Xu ◽  
Yi Li ◽  
Guanghui Gao
Keyword(s):  
Long Term Storage ◽  
Long Term Preservation ◽  
Term Storage ◽  
Storage Ability

A photochromic film that could be fast erased and possess long-term preservation ability has been designed, and could be used as rewritable paper.

scholarly journals CRYOPRESERVATION OF TRUE-SEED AND EMBRYO OF MAIZE AND SOYBEAN FOR LONG-TERM STORAGE

2016 ◽  
Vol 2 (2) ◽  
pp. 31
Author(s):  
Enny Sudarmonowati ◽  
I. Fitryatmi ◽  
S. Sadjad
Keyword(s):  
Liquid Nitrogen ◽  
Rapid Freezing ◽  
Soybean Seeds ◽  
Long Term Storage ◽  
Significant Difference ◽  
Long Term Preservation ◽  
Term Storage ◽  
Cryoprotectant Solution ◽  
Excised Embryos

<br />Study on cryopreservation of Indonesian local cultivars and improved  varieties of maize and soybean has never been done. This method may be used for long-term preservation of seeds of maize and soybean. In this study, the method was applied to maize and soybean, Arjuna and Wilis respectively, as a model for preserving germplasm of ortodox seeds. Whole seeds and excised embryos of both varieties were subjected to two methods of cryopreservation, i.e., two-stage cooling and rapid freezing with or without 15% dimethyl sulfoxide (DMSO) as cryoprotectant solution prior to immersion in liquid nitrogen (-196oC). Results indicated that there was no significant difference between the use of DMSO for both species in terms of viability, although pretreatment in DMSO was slightly reduced the percentage of viability of both species. Slow freezing to -30oC prior to immersion in the liquid nitrogen could give as high as 76.67% and 51.67% surviving whole seeds of maize and soybean, respectively. Preserving excised embryos of maize in the liquid nitrogen using either slow or rapid freezing significantly reduced the percentage of viability from 20-76.67% to 5-18.33% (four folds) depending on treatments applied. Results also showed that one day or 15 minutes of immersion of samples in the liquid nitrogen gave rise to similar values of viability of maize and soybean, i.e., 20-60% and 20-51.67%, respectively depending on  treatments applied. These results implied that for long-term storage of maize and soybean seeds as they could survive at the rate of 76.67% and 51.67% respectively, the seed can be treated by prefreezing to -30oC<br />without the presence of DMSO prior to immersion in liquid nitrogen.<br /><br />

Agglutinability of Red Cells After Long-Term Storage in the Frozen State

Transfusion ◽  
1962 ◽  
Vol 2 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Paul V. Strumia ◽  
Max M. Strumia ◽  
Louise S. Colwell ◽  
Barbara Torg
Keyword(s):  
Red Cells ◽  
Long Term Storage ◽  
Frozen State ◽  
Term Storage

scholarly journals THE USE OF DORMANT-BUD CRYOPRESERVATION FOR LONG-TERM STORAGE OF PECAN AND WALNUT GERMPLASM

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1142c-1142 ◽  
Author(s):  
Todd M. Morrissey ◽  
William A. Gustafson
Keyword(s):  
Woody Plants ◽  
Vaccinium Corymbosum ◽  
Black Walnut ◽  
Long Term Storage ◽  
Dormant Bud ◽  
If Current ◽  
Cryopreservation Techniques ◽  
Long Term Preservation ◽  
Term Storage

A study was designed to determine if current dormant-bud cryopreservation techniques investigated on woody plants, such as apple (Malus domestica), gooseberry (Ribes), blueberry (Vaccinium corymbosum) and pear (Pryus communis) etc., could be applied to certain nut tree species for long-term preservation. Pecan (Carya illinoinensis) and black walnut (Juglans nigra) were exposed to prefreezing temperatures ranging from -10° C to -40° C and then directly immersed in liquid nitrogen for 2 hrs. Dehydration by prefreezing was not sufficient for bud survival in pecan. Bud survival was increased by dehydrating stem sections prior to prefreezing. Prefreezing at -30° or -40° C was suitable for survival of black walnut.

The Stability and Shelf-Life of Liposome Encapsulated Hemoglobin: A Potential Blood Substitute

1987 ◽  
Vol 110 ◽  
Author(s):  
Alans Rudolph ◽  
Lewis P. Stratton ◽  
Wayne K. Knoll ◽  
Sandra Bayne ◽  
Frances Ligler
Keyword(s):  
Shelf Life ◽  
Blood Substitute ◽  
Oxidative Process ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Great Progress ◽  
The Stability ◽  
Long Term Preservation ◽  
Term Storage

AbstractFor any viable blood substitute, questions of long-term storage and shelf-life must be addressed. Recently, we have made great progress in improving the stability of the blood substitute, liposome encapsulated hemoglobin (LEH). We have concentrated our efforts on protecting LEH in solution and in the long-term preservation of LEH by lyophilization. In particular, we have been able to retard and in some cases, reverse the oxidative process of metHb formation in solution by the addition of antioxidants such as NADH and glutathione. We have been able to regenerate Hb preparations with 60% metHb by the addition of 10 mM NADH and glutathione. In these preparations addition of these antioxidants results in a decrease of metHb levels from 62% to 15% over the course of 12.5 days at 4°C. We have also explored the use of protective solutes such as the disaccharide trehalose in the preservation of LEH in the freeze-dried state. Addition of increasing amounts of trehalose and other disaccharides results in the inhibition of lyophilization-induced fusion events and in the retention of hemoglobin within the unilamellar liposomal vesicles following rehydration.

scholarly journals CRYOPRESERVATION OF TRUE-SEED AND EMBRYO OF MAIZE AND SOYBEAN FOR LONG-TERM STORAGE

2016 ◽  
Vol 2 (2) ◽  
pp. 31
Author(s):  
Enny Sudarmonowati ◽  
I. Fitryatmi ◽  
S. Sadjad
Keyword(s):  
Liquid Nitrogen ◽  
Rapid Freezing ◽  
Soybean Seeds ◽  
Long Term Storage ◽  
Significant Difference ◽  
Long Term Preservation ◽  
Term Storage ◽  
Cryoprotectant Solution ◽  
Excised Embryos

<br />Study on cryopreservation of Indonesian local cultivars and improved  varieties of maize and soybean has never been done. This method may be used for long-term preservation of seeds of maize and soybean. In this study, the method was applied to maize and soybean, Arjuna and Wilis respectively, as a model for preserving germplasm of ortodox seeds. Whole seeds and excised embryos of both varieties were subjected to two methods of cryopreservation, i.e., two-stage cooling and rapid freezing with or without 15% dimethyl sulfoxide (DMSO) as cryoprotectant solution prior to immersion in liquid nitrogen (-196oC). Results indicated that there was no significant difference between the use of DMSO for both species in terms of viability, although pretreatment in DMSO was slightly reduced the percentage of viability of both species. Slow freezing to -30oC prior to immersion in the liquid nitrogen could give as high as 76.67% and 51.67% surviving whole seeds of maize and soybean, respectively. Preserving excised embryos of maize in the liquid nitrogen using either slow or rapid freezing significantly reduced the percentage of viability from 20-76.67% to 5-18.33% (four folds) depending on treatments applied. Results also showed that one day or 15 minutes of immersion of samples in the liquid nitrogen gave rise to similar values of viability of maize and soybean, i.e., 20-60% and 20-51.67%, respectively depending on  treatments applied. These results implied that for long-term storage of maize and soybean seeds as they could survive at the rate of 76.67% and 51.67% respectively, the seed can be treated by prefreezing to -30oC<br />without the presence of DMSO prior to immersion in liquid nitrogen.<br /><br />

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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