Characterization of Carbon Nanotube-Conjugated Collagen Composite Matrix Mechanics

2007 ◽  
Author(s):  
John R. Twomey ◽  
Vivek Sundaram ◽  
Krishna Madhavan ◽  
Wei Tan
Keyword(s):  
Mechanical Properties ◽  
Type I Collagen ◽  
High Stress ◽  
Type I ◽  
Composite Matrix ◽  
Matrix Mechanics ◽  
Tissue Constructs ◽  
Engineered Tissue ◽  
Walled Carbon Nanotubes

In the case of vascular grafts, enhanced mechanical properties of engineered tissue constructs are required in order to function properly in mechanically-active physiologic conditions. It is proposed that a composite matrix constructed of type I collagen, fibronectin, and covalently-functionalized single-walled carbon nanotubes (SWNTs) will provide the desired mechanical properties required for the development of implantable tissues capable of withstanding high-stress environments.

Magnetic Alignment of Type I Collagen as a Method for Altering Tensile Mechanical Properties

2012 ◽  
Author(s):  
Tyler Novak ◽  
Jamie Canter ◽  
Dafang Chen ◽  
Joel Hungate ◽  
Sherry Voytik-Harbin ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Type I Collagen ◽  
Structural Integrity ◽  
Biological Response ◽  
Type I ◽  
Noninvasive Methods ◽  
Tissue Constructs ◽  
Musculoskeletal Tissues ◽  
Common Technique ◽  
Scaffold Materials

To date, ligament and tendon replacements largely utilize autograft/allograft transplantation, although the use of tissue engineered materials remain a promising solution [10]. The development of an engineered solution may depend on the choice of scaffold materials with optimal fiber alignment. Type I collagen is an abundant extracellular matrix component in musculoskeletal tissues. The controlled alignment of type I collagen for tissue engineering and regenerative medicine applications enables the fabrication of unique scaffolds that emulate the ultrastructure of their native counterparts. Moreover, the alignment of type I collagen has become a common technique to manipulate mechanical properties of tissue constructs and the biological response of embedded cells [1,2]. It is additionally important to develop noninvasive methods to align collagen structures while maintaining inherent structural integrity and biological activity.

Intrinsic mechanical properties of the extracellular matrix affect the behavior of pre-osteoblastic MC3T3-E1 cells

2006 ◽  
Vol 290 (6) ◽  
pp. C1640-C1650 ◽  
Author(s):  
Chirag B. Khatiwala ◽  
Shelly R. Peyton ◽  
Andrew J. Putnam
Keyword(s):  
Mechanical Properties ◽  
Type I Collagen ◽  
Focal Adhesions ◽  
Microscopic Analysis ◽  
Maximal Activity ◽  
Type I ◽  
Cell Functions ◽  
Collagen Density ◽  
Cells Cultured ◽  
In Cells

Mechanical cues present in the ECM have been hypothesized to provide instructive signals that dictate cell behavior. We probed this hypothesis in osteoblastic cells by culturing MC3T3-E1 cells on the surface of type I collagen-modified hydrogels with tunable mechanical properties and assessed their proliferation, migration, and differentiation. On gels functionalized with a low type I collagen density, MC3T3-E1 cells cultured on polystyrene proliferated twice as fast as those cultured on the softest substrate. Quantitative time-lapse video microscopic analysis revealed random motility speeds were significantly retarded on the softest substrate (0.25 ± 0.01 μm/min), in contrast to maximum speeds on polystyrene substrates (0.42 ± 0.04 μm/min). On gels functionalized with a high type I collagen density, migration speed exhibited a biphasic dependence on ECM compliance, with maximum speeds (0.34 ± 0.02 μm/min) observed on gels of intermediate stiffness, whereas minimum speeds (0.24 ± 0.03 μm/min) occurred on both the softest and most rigid (i.e., polystyrene) substrates. Immature focal contacts and a poorly organized actin cytoskeleton were observed in cells cultured on the softest substrates, whereas those on more rigid substrates assembled mature focal adhesions and robust actin stress fibers. In parallel, focal adhesion kinase (FAK) activity (assessed by detecting pY397-FAK) was influenced by compliance, with maximal activity occurring in cells cultured on polystyrene. Finally, mineral deposition by the MC3T3-E1 cells was also affected by ECM compliance, leading to the conclusion that altering ECM mechanical properties may influence a variety of MC3T3-E1 cell functions, and perhaps ultimately, their differentiated phenotype.

Assembly of type I collagen: fusion of fibril subunits and the influence of fibril diameter on mechanical properties

2000 ◽  
Vol 19 (5) ◽  
pp. 409-420 ◽  
Author(s):  
David L. Christiansen ◽  
Eric K. Huang ◽  
Frederick H. Silver
Keyword(s):  
Mechanical Properties ◽  
Type I Collagen ◽  
Type I ◽  
Fibril Diameter

scholarly journals Mechanical Properties of Native and Cross-linked Type I Collagen Fibrils

2008 ◽  
Vol 94 (6) ◽  
pp. 2204-2211 ◽  
Author(s):  
Lanti Yang ◽  
Kees O. van der Werf ◽  
Carel F.C. Fitié ◽  
Martin L. Bennink ◽  
Pieter J. Dijkstra ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Type I Collagen ◽  
Collagen Fibrils ◽  
Type I

scholarly journals Regenerative Rehabilitation in Injuries of Tendons

2021 ◽  
Vol 3 (2) ◽  
pp. 192-206
Author(s):  
Sergey G. Sсherbak ◽  
Stanislav V. Makarenko ◽  
Olga V. Shneider ◽  
Tatyana A. Kamilova ◽  
Alexander S. Golota
Keyword(s):  
Mechanical Properties ◽  
Type I Collagen ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Tendon Healing ◽  
Tendon Injuries ◽  
Type I ◽  
Differentiation Markers ◽  
Postoperative Rehabilitation ◽  
Potent Inducer

The mechanical properties of tendons are thought to be affected by different loading levels. Changes in the mechanical properties of tendons, such as stiffness, have been reported to influence the risk of tendon injuries chiefly in athletes and the elderly, thereby affecting motor function execution. Unloading resulted in reduced tendons stiffness, and resistance exercise exercise counteracts this. Transforming growth factor-1 is a potent inducer of type I collagen and mechanosensitive genes encoding tenogenic differentiation markers expression which play critical roles in tendon tissue formation, tendon healing and their adaptation during exercise. In recent years, our understanding of the molecular biology of tendons growth and repair has expanded. It is probable that the next advance in the treatment of tendon injuries will result from the application of this basic science knowledge and the clinical solution will encompass not only the the best postoperative rehabilitation protocols, but also the optimal biological modulation of the healing process.

Sub‐ and Supramolecular X‐Ray Characterization of Engineered Tissues from Equine Tendon, Bovine Dermis, and Fish Skin Type‐I Collagen

2020 ◽  
Vol 20 (5) ◽  
pp. 2000017 ◽  
Author(s):  
Alberta Terzi ◽  
Nunzia Gallo ◽  
Simona Bettini ◽  
Teresa Sibillano ◽  
Davide Altamura ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Skin Type ◽  
Type I ◽  
Fish Skin ◽  
X Ray ◽  
Engineered Tissues
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