Molecular Delivery Into Live Cells Using Gold Nanoparticle Arrays Fabricated by Polymer Mold Guided Near-Field Photothermal Annealing

2010 ◽  
Author(s):  
Ting-Hsiang Wu ◽  
Fan Xiao ◽  
Sheraz Kalim ◽  
Tara Teslaa ◽  
Michael A. Teitell ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Gold Nanoparticle ◽  
Targeted Delivery ◽  
Mammalian Cells ◽  
Near Field ◽  
Cell Monolayer ◽  
Nanoparticle Arrays ◽  
Molecular Delivery ◽  
A Cell ◽  
Polymer Mold

A massively-parallel molecular delivery system for mammalian cells is demonstrated by pulsed-laser irradiation of a gold nanoparticle array situated below a cell monolayer. This system is capable of high throughput and spatially-targeted delivery into desired areas of a cell culture by designing the laser irradiation pattern. Rapid fabrication of gold nanoparticle arrays over large areas (>1 mm2) is achieved by polymer mold guided near-field photothermal annealing.

scholarly journals Near-field heat transfer between gold nanoparticle arrays

2013 ◽  
Vol 114 (21) ◽  
pp. 214306 ◽  
Author(s):  
Anh D. Phan ◽  
The-Long Phan ◽  
Lilia M. Woods
Keyword(s):  
Heat Transfer ◽  
Gold Nanoparticle ◽  
Near Field ◽  
Nanoparticle Arrays

Dielectrophoretic Assembly of Gold Nanoparticle Arrays Evaluated in Terms of Room-Temperature Resistance

2020 ◽  
Vol E103.C (2) ◽  
pp. 62-65
Author(s):  
Yoshinao MIZUGAKI ◽  
Makoto MORIBAYASHI ◽  
Tomoki YAGAI ◽  
Masataka MORIYA ◽  
Hiroshi SHIMADA ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Room Temperature ◽  
Nanoparticle Arrays ◽  
Temperature Resistance

Analysis of the Dynamics of the Electric Capacitance of a Cell Monolayer at the Late Stages of Caco-2 cell Differentiation

2019 ◽  
Vol 35 (6) ◽  
pp. 87-90
Author(s):  
S.V. Nikulin ◽  
V.A. Petrov ◽  
D.A. Sakharov
Keyword(s):  
Impedance Spectroscopy ◽  
Cell Monolayer ◽  
Microfluidic Chips ◽  
Russian Science ◽  
Electric Capacitance ◽  
A Cell ◽  
Static Conditions ◽  
Late Stages

The real-time monitoring of electric capacitance (impedance spectroscopy) allowed obtaining evidence that structures which look like intestinal villi can be formed during the cultivation under static conditions as well as during the cultivation in microfluidic chips. It was shown in this work via transcriptome analysis that the Hh signaling pathway is involved in the formation of villus-like structures in vitro, which was previously shown for their formation in vivo. impedance spectroscopy, intestine, villi, electric capacitance, Hh The study was funded by the Russian Science Foundation (Project 16-19-10597).

Seed-Mediated Growth of Uniform Gold Nanoparticle Arrays

2007 ◽  
Vol 111 (35) ◽  
pp. 12933-12938 ◽  
Author(s):  
Sachin Kumar ◽  
Hongzhou Yang ◽  
Shouzhong Zou
Keyword(s):  
Gold Nanoparticle ◽  
Nanoparticle Arrays ◽  
Seed Mediated

Sterol and lipid trafficking in mammalian cells

2006 ◽  
Vol 34 (3) ◽  
pp. 335-339 ◽  
Author(s):  
F.R. Maxfield ◽  
M. Mondal
Keyword(s):  
Membrane Trafficking ◽  
Mammalian Cells ◽  
Intracellular Transport ◽  
Vesicular Transport ◽  
Cholesterol Content ◽  
Cellular Functions ◽  
Lipid Trafficking ◽  
Sterol Transport ◽  
A Cell ◽  
Asymmetrically Distributed

The pathways involved in the intracellular transport and distribution of lipids in general, and sterols in particular, are poorly understood. Cholesterol plays a major role in modulating membrane bilayer structure and important cellular functions, including signal transduction and membrane trafficking. Both the overall cholesterol content of a cell, as well as its distribution in specific organellar membranes are stringently regulated. Several diseases, many of which are incurable at present, have been characterized as results of impaired cholesterol transport and/or storage in the cells. Despite their importance, many fundamental aspects of intracellular sterol transport and distribution are not well understood. For instance, the relative roles of vesicular and non-vesicular transport of cholesterol have not yet been fully determined, nor are the non-vesicular transport mechanisms well characterized. Similarly, whether cholesterol is asymmetrically distributed between the two leaflets of biological membranes, and if so, how this asymmetry is maintained, is poorly understood. In this review, we present a summary of the current understanding of these aspects of intracellular trafficking and distribution of lipids, and more specifically, of sterols.

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