Effects of Ultrasonic Vibration-Assisted Pelleting on Sugar Yield in Biofuel Manufacturing With Different Pretreatment Methods

2013 ◽  
Author(s):  
Elizabeth Kennedy ◽  
Pengfei Zhang ◽  
Qi Zhang ◽  
Z. J. Pei ◽  
Donghai Wang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ultrasonic Vibration ◽  
Large Scale ◽  
Sugar Yield ◽  
Hot Water ◽  
Dilute Acid Pretreatment ◽  
Cellulosic Biofuels ◽  
Acid Pretreatment ◽  
Promising Alternative ◽  
Low Efficiency

There are many concerns taking place due to the reliability and sustainability of petroleum-based liquid transportation fuels. The importance of finding an alternative fuel source to the petroleum-based fuels is in high demand. One promising alternative is cellulosic biofuels that offer numerous benefits for the environment. However, there are some obstacles in the way of manufacturing the cellulosic biofuels at a large scale and doing so cost effectively. One obstacle is the low sugar yield of biomass in enzymatic hydrolysis, leading to low efficiency in biomass-biofuel conversion and thus high conversion costs. Pretreatment of biomass is required to achieve high sugar yield in enzymatic hydrolysis. Dilute acid pretreatment and hot water pretreatment can effectively increase the sugar yield, but they usually involve high temperature or excessive use of acid, making pretreatment one of the most costly process in cellulosic biofuel manufacturing. This study investigated effects of ultrasonic vibration-assisted pelleting on the sugar yield of biomass pretreated by the two pretreatment methods. Results showed that, for both pretreatment methods, pellets produces higher sugar yield than particles. Furthermore, at the same pretreatment temperature, the water-pretreated pellets produced higher sugar yield than acid-pretreated particles.

Ultrasonic Vibration-Assisted Pelleting of Sorghum Stalks: Effects of Pressure and Ultrasonic Power

2010 ◽  
Author(s):  
Qi Zhang ◽  
P. F. Zhang ◽  
Timothy Deines ◽  
Z. J. Pei ◽  
Donghai Wang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ultrasonic Vibration ◽  
Large Scale ◽  
Cost Effective ◽  
Sugar Yield ◽  
Cellulosic Biomass ◽  
Ultrasonic Power ◽  
Cellulosic Biofuels ◽  
Transportation Fuels ◽  
Low Efficiency

Cellulosic biofuels can be used to replace traditional liquid transportation fuels. Cellulosic biomass is feedstock in manufacturing of cellulosic biofuels. However, the low density of cellulosic biomass feedstock hinders large-scale and cost-effective manufacturing of cellulosic biofuels. Another bottleneck factor in manufacturing of cellulosic biofuels is the low efficiency of the enzymatic hydrolysis of cellulosic biomass materials resulting in a low sugar yield. Ultrasonic vibration-assisted (UV-A) pelleting can increase the density of cellulosic biomass feedstocks via combined effects of mechanical compression and ultrasonic vibration of the tool on the cellulosic biomass. Meanwhile ultrasonic vibration may act as a beneficial pretreatment for enzymatic hydrolysis, which can possibly increase the efficiency of hydrolysis and obtain a higher sugar yield. The pressure and the ultrasonic power are important parameters in UV-A pelleting. Their effects on pellet quality (density, durability, and stability) and sugar yield (after hydrolysis) are experimentally investigated.

Ultrasonic Vibration-Assisted Pelleting of Biomass: A Designed Experimental Investigation on Pellet Quality and Sugar Yield

2010 ◽  
Author(s):  
Pengfei Zhang ◽  
Timothy Deines ◽  
Daniel Nottingham ◽  
Z. J. Pei ◽  
Donghai Wang ◽  
...  
Keyword(s):  
Experimental Investigation ◽  
Ultrasonic Vibration ◽  
Large Scale ◽  
Sugar Yield ◽  
Cellulosic Biomass ◽  
Ultrasonic Power ◽  
Process Variables ◽  
Cellulosic Biofuels ◽  
Pellet Quality ◽  
Low Efficiency

Increasing demands and concerns for the reliable supply of liquid transportation fuels make it important to find alternative sources to petroleum based fuels. One such alternative is cellulosic biofuels. However, several technical barriers have hindered large-scale, cost-effective manufacturing of cellulosic biofuels, such as the low density of cellulosic feedstocks (causing high transportation and storage costs) and the low efficiency of enzymatic hydrolysis process (causing longer processing time and low sugar yield). Ultrasonic vibration-assisted (UV-A) pelleting can increase the density of cellulosic materials by compressing them into pellets. UV-A pelleting can also increase the sugar yield of cellulosic biomass materials in hydrolysis. At present, the effects of process variables in UV-A pelleting on pellet quality (density, durability, and stability) and sugar yield have not been adequately investigated. This paper reports an experimental investigation on UV-A pelleting of wheat straw. A 24 factorial design is employed to evaluate the effects of process variables (moisture content, particle size, pelleting pressure, and ultrasonic power) on output variables (pellet density, durability, stability, and sugar yield).

Dilute Acid Pretreatment and Enzymatic Hydrolysis of Woody Biomass for Biofuel Manufacturing: Effects of Particle Size on Sugar Conversion

2013 ◽  
Author(s):  
Meng Zhang ◽  
Xiaoxu Song ◽  
Pengfei Zhang ◽  
Z. J. Pei
Keyword(s):  
Weight Loss ◽  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Sugar Yield ◽  
Total Sugar ◽  
Cellulosic Biomass ◽  
Dilute Acid Pretreatment ◽  
Promising Alternative ◽  
Biomass Particle Size ◽  
Sugar Conversion

Biofuels derived from cellulosic biomass offer a promising alternative to petroleum-based liquid transportation fuels. Cellulosic biomass can be converted into biofuels through biochemical pathway. This pathway consists of two major conversions: sugar conversion and ethanol conversion. Sugar yield in sugar conversion is critical to the cost effectiveness of biofuel manufacturing, because it is approximately proportional to the ethanol biofuel yield. Cellulosic biomass sugar conversion consists of pretreatment and enzymatic hydrolysis. Biomass particle size is an important factor affecting sugar yield. The literature contains many studies investigating the relationship between particle size and sugar yield. Many studies focused only on the sugar yield in enzymatic hydrolysis, and failed to take into account the biomass weight loss during pretreatment. This weight loss results in a loss of the amount of potential sugar (cellulose), which continues going into enzymatic hydrolysis. Without considering this loss, cellulosic biomass with a higher enzymatic hydrolysis sugar yield may end up with a lower total sugar yield through sugar conversion. The present study aims to address this issue by investigating the effects of biomass particle size using total sugar yield, a parameter considering both the biomass weight loss in pretreatment and the sugar yield in enzymatic hydrolysis.

Effects of Ultrasonic Treatments on Cellulose in Cellulosic Biofuel Manufacturing: A Literature Review

2010 ◽  
Author(s):  
P. F. Zhang ◽  
Z. J. Pei
Keyword(s):  
Large Scale ◽  
Morphological Structure ◽  
Degree Of Polymerization ◽  
Sugar Yield ◽  
Cellulosic Biomass ◽  
Cellulosic Biofuels ◽  
Crystallinity Degree ◽  
Transportation Fuels ◽  
Cellulosic Biofuel ◽  
Low Efficiency

Cellulosic biofuels are one type of renewable energy, and have been proposed to replace traditional liquid transportation fuels. Cellulosic biomass is the feedstocks in cellulosic biofuel manufacturing. Cellulose accounts for approximately 30% of the total weight in cellulosic biomass. Glucose, one type of monosaccharide convertible to ethanol, can be obtained by hydrolyzing the polymeric structure of cellulose. Currently enzymatic methods are the most common for the hydrolysis of cellulose. However, the low efficiency of enzymatic hydrolysis increases production cost and hinders the large-scale manufacturing of cellulosic biofuels. Ultrasonic treatments applied on cellulosic biomass were found to improve the efficiency of hydrolysis and subsequently increase the sugar yield of hydrolysis. To understand the effects of ultrasonics on cellulose, investigations have been conducted on the effects on cellulose characteristics caused by ultrasonic treatments during hydrolysis. This paper reviews the effects of ultrasonic treatments on cellulose during hydrolysis in terms of sugar yield and some characteristics of cellulose, such as accessibility, crystallinity, degree of polymerization, and morphological structure.

Ultrasonic Vibration-Assisted Pelleting in Manufacturing of Cellulosic Biofuels: An Investigation on Biomass Particle Size

2011 ◽  
Author(s):  
Pengfei Zhang ◽  
Qi Zhang ◽  
Z. J. Pei ◽  
Linda Pei
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Ultrasonic Vibration ◽  
Sugar Yield ◽  
Cellulosic Biomass ◽  
Particle Sizes ◽  
Cellulosic Biofuels ◽  
Significant Difference ◽  
Before And After ◽  
Biomass Particle Size

Biofuels made from cellulosic biomass are an alternative to petroleum-based liquid transportation fuels. However, several technical barriers have hindered cost-effective manufacturing of cellulosic biofuels, such as low density of cellulosic feedstocks (causing high transportation and storage costs) and low sugar yield in enzymatic hydrolysis. Ultrasonic vibration-assisted (UV-A) pelleting can increase the density of cellulosic feedstocks and the sugar yield in enzymatic hydrolysis. A hypothesis for UV-A pelleting to increase sugar yield is that UV-A pelleting reduces the particle size of cellulosic biomass. This paper reports an experimental investigation on effects of UV-A pelleting on biomass particle size. Particle sizes before and after UV-A pelleting are measured and compared. The results show that there is no significant difference between particle sizes before and after UV-A pelleting. Therefore, the hypothesis is rejected.

scholarly journals Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Dilute Acid Pretreatment ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Organic Reagents ◽  
Milled Wood Lignin ◽  
Affinity Constants

Abstract Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

scholarly journals Investigation of interaction between cellulase and residual lignin fractions in acid-pretreated bamboo residues and their effect on enzymatic digestibility

2021 ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Interaction Mechanism ◽  
Dilute Acid Pretreatment ◽  
Affinity Constant ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Abstract Background: During dilute acid pretreatment, pseudo lignin and lignin form droplets which deposit on the surface of lignocellulose, and further inhibit its enzymatic hydrolysis. However, how this lignin interacts with cellulase enzymes and then affects enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by milled wood lignin (MWL) method. All the obtained lignin fractions from DAP-BR were used to investigate the interaction mechanism between lignin and cellulase using surface plasmon resonance (SPR) technology in order to understand how they affect enzymatic hydrolysisResults: Results showed that removing surface lignin significantly decrease the enzymatic hydrolysis of DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel decreased enzymatic hydrolysis of Avicel, while different SL samples showed a slight increase to its enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus the SL samples, stronger affinity for MWL (KD = 6.8-24.7 nM) was found versus that of SL (KD = 39.4-52.6 nM) by SPR analysis. The affinity constant of all tested lignin had good correlations (R2>0.6) with their effects on enzymatic digestibility of extracted DAP-BR and Avicel.Conclusions: This work reveals that the surface lignin on DAP-BR is necessary towards maintaining enzyme digestibility levels, and its removal has a negative impact on the substrate’s digestibility.

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

scholarly journals Evaluation of the Interactive Effect Pretreatment Parameters via Three Types of Microwave-Assisted Pretreatment and Enzymatic Hydrolysis on Sugar Yield

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 787 ◽  
Author(s):  
Saleem Ethaib ◽  
Rozita Omar ◽  
Mustapa Kamal Siti Mazlina ◽  
Awang Biak Dayang Radiah
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Interactive Effect ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Solid Loading ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Sago Palm ◽  
Microwave Assisted

This study aims to evaluate the sugar yield from enzymatic hydrolysis and the interactive effect pretreatment parameters of microwave-assisted pretreatment on glucose and xylose. Three types of microwave-assisted pretreatments of sago palm bark (SPB) were conducted for enzymatic hydrolysis, namely: microwave-sulphuric acid pretreatment (MSA), microwave-sodium hydroxide pretreatment (MSH), and microwave-sodium bicarbonate (MSB). The experimental design was done using a response surface methodology (RSM) and Box–Behenken Design (BBD). The pretreatment parameters ranged from 5–15% solid loading (SL), 5–15 min of exposure time (ET), and 80–800 W of microwave power (MP). The results indicated that the maximum total reducing sugar was 386 mg/g, obtained by MSA pretreatment. The results also illustrated that the higher glucose yield, 44.3 mg/g, was found using MSH pretreatment, while the higher xylose yield, 43.1 mg/g, resulted from MSA pretreatment. The pretreatment parameters MP, ET, and SL showed different patterns of influence on glucose and xylose yield via enzymatic hydrolysis for MSA, MSH, and MSB pretreatments. The analyses of the interactive effect of the pretreatment parameters MP, ET, and SL on the glucose yield from SPB showed that it increased with the high MP and longer ET, but this was limited by low SL values. However, the analysis of the interactive effect of the pretreatment parameters on xylose yields revealed that MP had the most influence on the xylose yield for MSA, MSH, and MSB pretreatments.

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