Preparation and Characterization of Tricolor CdSe-Tagged Microbeads for Bio-Detection

Author(s):  
Jin Chang ◽  
Bingbo Zhang ◽  
Dena Li ◽  
Guiping Ma ◽  
Weicai Wang ◽  
...  

Tricolor microbeads for biological assay have been prepared by embedding three quantum dots (cadmium selenide semiconductor nanocrystals) of different size into carboxyl-functionalized polystyrene (PS-COOH) microbeads. These efforts can render CdSe nanocrystals water-solubility, chemical stability and good photostability. The results indicate that QDs-tagged microbeads are highly uniform, reproducible and strong in fluorescence emission. Based on the properties it possesses, QDs-tagged microbead may have great potential for bio-detection.

2013 ◽  
Vol 117 (44) ◽  
pp. 22509-22517 ◽  
Author(s):  
Irene Barceló ◽  
Elena Guillén ◽  
Teresa Lana-Villarreal ◽  
Roberto Gómez

2013 ◽  
Vol 67 (9) ◽  
pp. 997-1002 ◽  
Author(s):  
Viviane Pilla ◽  
Leandro P. Alves ◽  
Adalberto N. Iwazaki ◽  
Acácio A. Andrade ◽  
Andrea Antunes ◽  
...  

Author(s):  
Chu Viet Ha ◽  
Chu Anh Tuan ◽  
Nguyen Thi Bich Ngoc ◽  
Tran Hong Nhung ◽  
Nguyen Quang Liem ◽  
...  

The quantum dots coated by silica  is fluorescence material class with great biocompatibility, low toxicity and water-solubility, that is suitable for bioapplications. This work presents the synthesis of SiO2 coated CdTe/ZnSe (named CdTe) quantum dots (CdTe@SiO2 nanoparticles) via a wet chemmical route called modified Stöber method. The compounds tetraethylorthosilicate (TEOS) has used as precursors, aminopropyltriethoxysilane (APTES) is  as  electric neutralizer, and ammonium hydroxide is used as catalysts. The size of CdTe@SiO2 nanoparticles was estimated about 70 to 150 nm depending on the quantities of H2O, APTEOS, and catalysts. The emission behaviours of SiO2 coated quantum dots was effected by ratio of substances participating in the reaction and synthesis conditions. with the ratio (by volume) of suitable substances: TEOS:solution of QDs:NH4OH:APTES:H2O being 1.5:1.5x10-2:0.8x10-2:4x10-2:3x10-4:5x10-2, the prepared silica nanoparticles containing quantum dots show high fluorescence emission efficiency, the fluorescence intensity is higher than that of uncoated CdTe/ZnSe quantum dots. This is a positive result in the technique of manufacturing luminescent silica nanoparticles containing quantum dots. The results show an ability to use the CdTe@SiO2 nanoparticles for biological application.


2019 ◽  
Vol 21 (29) ◽  
pp. 16108-16119 ◽  
Author(s):  
F. A. Zhao ◽  
H. Y. Xiao ◽  
X. M. Bai ◽  
X. T. Zu

Cadmium selenide (CdSe) nanocrystals are important photoelectric materials.


2001 ◽  
Vol 704 ◽  
Author(s):  
D.M. Speckman ◽  
T.L. Jennings ◽  
S.D. LaLumondiere ◽  
S.C. Moss

AbstractSemiconductor nanocrystals are expected to play an important role in the development of novel electronic and optoelectronic devices, and have already shown promise in the area of biological reporters for medical screening and sensor applications. We have been involved in developing luminescent CdSe/ZnS core-shell nanocrystals (or quantum dots, QDs) for use in self-assembled structures and as fluorescent reporters in immunoassay-based biodetectors. As part of our efforts to bind semiconductor CdSe/ZnS quantum dots to antibody proteins for our immunoassay work, we functionalized the nanocrystal surfaces with a variety of organic acid salts to impart water solubility to the nanocrystals.During the course of working with these derivatized, water-soluble quantum dots, we observed significant differences in their chemical reactivities and physical characteristics compared to those of underivatized CdSe/ZnS nanocrystals. One of the most striking differences observed is the reactivity of the derivatized and underivatized nanocrystals with stainless steel surfaces. The fluorescence of aqueous mixtures of our water-soluble nanocrystals is immediately quenched upon exposure of the mixtures to stainless steel (SS) surfaces or to chromium oxide, whereas underivatized quantum dots exhibit little or no reactivity at all. As has been reported by several other laboratories, the water-soluble nanocrystals also exhibit significantly lower quantum yields compared to the underivatized nanocrystals. We discuss the unusual reactivity exhibited by these nanocrystals, and suggest possible explanations for their interesting chemical behavior. We also describe methods to prevent the quenching of water-soluble derivatized quantum dots by stainless steel and metal oxides.


Nanoscale ◽  
2012 ◽  
Vol 4 (15) ◽  
pp. 4719 ◽  
Author(s):  
Claire Gégout ◽  
Maria L. McAtee ◽  
Nichole M. Bennett ◽  
L. M. Viranga Tillekeratne ◽  
Jon R. Kirchhoff

2017 ◽  
Vol 4 (1) ◽  
pp. 244-254 ◽  
Author(s):  
Shauhrat S. Chopra ◽  
Thomas L. Theis

Quantum dots (QDs) are semiconductor nanocrystals (2–10 nm) with tunable band gaps and desirable luminescence properties.


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