3D Reconstruction Based on Single Defocused Microscopic Image
Microscope has being limited by the depth of focus, while the focused image is clear, the defocused images are fuzzy and fuzzy degree of the object images vary with different defocused distances. This paper presented a 3D reconstruction method based on a defocused microscopic image. After the defocused microscopic image is divided the microscopic into M × N regions, the fuzzy degree of each region is quantitatively evaluated. A corresponding curve of the relation between fuzzy degree and defocus distance is drawn by the presented algorithm in this paper, and then the three-dimensional characteristics of objects are reconstructed. This method has the merits of little computation, low cost and high speed. And M and N values can be changed according to the needs of the measurement accuracy.