The Human Body is a Pressurized Self-Assembly Machine

2012 ◽  
Author(s):  
Brenda K. Krkosska Bayles
Keyword(s):  
Blood Pressure ◽  
Human Body ◽  
Self Assembly ◽  
The Novel ◽  
Daughter Cells ◽  
Self Assembling ◽  
Living Tissues ◽  
First Time

The novel and self-obtained concept of this paper is that living tissues get help self-assembling by following some mechanical equations. The simple diagnostic act of checking someone’s blood pressure reminds us that the human body is a pressurized object, and blood moving throughout the pressurized body creates flows that are strikingly similar to the movements of fluids in pressurized machines. Self-assembly using two mechanical concepts and their equations is herein demonstrated for the first time to show the separation of healthy daughter cells and the nonseparation of aneuploid cells, and to show monosaccharide and disaccharide movements.

scholarly journals Self-Assembly and Photophysical Studies of an Unusual Red Colored Dye Which Show Green Fluorescence in Cell Imaging

2021 ◽  
Author(s):  
Vivekshinh Kshtriya ◽  
Bharti Koshti ◽  
Ashadul Haque ◽  
Ankit Gangrade ◽  
Ramesh Singh ◽  
...  
Keyword(s):  
Self Assembly ◽  
Cell Imaging ◽  
Uv Light ◽  
Photophysical Properties ◽  
Unexpected Result ◽  
Green Fluorescence ◽  
Self Assembling ◽  
Red Color ◽  
Orange Colour ◽  
First Time

<p>We report for the very first time self-assembly of a red color dye 7-Amino-6h-anthra[9,1-cd][1,2]thiazol-6-one (<b>AAT</b>),its photophysical properties and its applications in cell imaging<b>.</b>Interestingly, <b>AAT </b>show intense red colour in visible light while it shows the orange colour fluorescence under UV light @312nm.Surprisingly, when this dye was used as cell imaging agent it revealed only green fluorescence inside cells and not red. Hence, the photophysical properties of this dye was very intriguing. Further, when self-assembling properties of this dye was examined it revealed formation of tree like branched structures which appeared red both under green and red filter which was again an unexpected result..Interestingly <b>AAT</b> self-assembly also show morphological transition and the branched tree like structures changes to straight fibres as the solvent is changed from DMSO to THF. Hence, the results of self-assembly and cell imaging were contrary to each other and the photophysical properties of this dye is very unusual as compared to conventional dyes. Our future endeavours willaim to understand this anomalous behaviour in greater details in future through various biophysical assays.</p>

scholarly journals Self-Assembly and Photophysical Studies of an Unusual Red Colored Dye Which Show Green Fluorescence in Cell Imaging

2021 ◽  
Author(s):  
Vivekshinh Kshtriya ◽  
Bharti Koshti ◽  
Ashadul Haque ◽  
Ankit Gangrade ◽  
Ramesh Singh ◽  
...  
Keyword(s):  
Self Assembly ◽  
Cell Imaging ◽  
Uv Light ◽  
Photophysical Properties ◽  
Unexpected Result ◽  
Green Fluorescence ◽  
Self Assembling ◽  
Red Color ◽  
Orange Colour ◽  
First Time

<p>We report for the very first time self-assembly of a red color dye 7-Amino-6h-anthra[9,1-cd][1,2]thiazol-6-one (<b>AAT</b>),its photophysical properties and its applications in cell imaging<b>.</b>Interestingly, <b>AAT </b>show intense red colour in visible light while it shows the orange colour fluorescence under UV light @312nm.Surprisingly, when this dye was used as cell imaging agent it revealed only green fluorescence inside cells and not red. Hence, the photophysical properties of this dye was very intriguing. Further, when self-assembling properties of this dye was examined it revealed formation of tree like branched structures which appeared red both under green and red filter which was again an unexpected result..Interestingly <b>AAT</b> self-assembly also show morphological transition and the branched tree like structures changes to straight fibres as the solvent is changed from DMSO to THF. Hence, the results of self-assembly and cell imaging were contrary to each other and the photophysical properties of this dye is very unusual as compared to conventional dyes. Our future endeavours willaim to understand this anomalous behaviour in greater details in future through various biophysical assays.</p>

scholarly journals Gas barrier enhancement of uncharged apolar polymeric films by self-assembling stratified nano-composite films

RSC Advances ◽  
2019 ◽  
Vol 9 (19) ◽  
pp. 10938-10947 ◽  
Author(s):  
Ali Akbar Motedayen ◽  
Mohammadreza Rezaeigolestani ◽  
Carole Guillaume ◽  
Valérie Guillard ◽  
Nathalie Gontard
Keyword(s):  
Self Assembly ◽  
Hydrophobic Interactions ◽  
Composite Films ◽  
The Self ◽  
Polymeric Films ◽  
Assembly Process ◽  
Gas Barrier ◽  
Nano Composite ◽  
Self Assembling ◽  
First Time

The gas (O2 and CO2) permeability of an innovative stratified PE–organoclay (LLDPE/OMMT) nano-enabled composite films was studied for the first time and related to the self-assembly process driven by hydrophobic interactions.

Buried Narratives: Exhuming the Mother's Story in Oliver Twist

2018 ◽  
Vol 8 (3) ◽  
pp. 247-266
Author(s):  
Michelle L. Wilson
Keyword(s):  
Social Change ◽  
Maternal Inheritance ◽  
The Novel ◽  
Poor Law ◽  
The Family ◽  
The Law ◽  
First Time ◽  
Critical Consideration

Initially, Oliver Twist (1839) might seem representative of the archetypal male social plot, following an orphan and finding him a place by discovering the father and settling the boy within his inheritance. But Agnes Fleming haunts this narrative, undoing its neat, linear transmission. This reconsideration of maternal inheritance and plot in the novel occurs against the backdrop of legal and social change. I extend the critical consideration of the novel's relationship to the New Poor Law by thinking about its reflection on the bastardy clauses. And here, of course, is where the mother enters. Under the bastardy clauses, the responsibility for economic maintenance of bastard children was, for the first time, legally assigned to the mother, relieving the father of any and all obligation. Oliver Twist manages to critique the bastardy clauses for their release of the father, while simultaneously embracing the placement of the mother at the head of the family line. Both Oliver and the novel thus suggest that it is the mother's story that matters, her name through which we find our own. And by containing both plots – that of the father and the mother – Oliver Twist reveals the violence implicit in traditional modes of inheritance in the novel and under the law.

Factors Affecting Molecular Self-Assembly and Its Mechanism

2012 ◽  
Vol 9 (1) ◽  
pp. 43 ◽  
Author(s):  
Hueyling Tan
Keyword(s):  
Self Assembly ◽  
Building Blocks ◽  
Molecular Engineering ◽  
Molecular Structures ◽  
Polymer Science ◽  
New Approach ◽  
Factors Affecting ◽  
Dna Structures ◽  
Self Assembling ◽  
Wide Range

Molecular self-assembly is ubiquitous in nature and has emerged as a new approach to produce new materials in chemistry, engineering, nanotechnology, polymer science and materials. Molecular self-assembly has been attracting increasing interest from the scientific community in recent years due to its importance in understanding biology and a variety of diseases at the molecular level. In the last few years, considerable advances have been made in the use ofpeptides as building blocks to produce biological materials for wide range of applications, including fabricating novel supra-molecular structures and scaffolding for tissue repair. The study ofbiological self-assembly systems represents a significant advancement in molecular engineering and is a rapidly growing scientific and engineering field that crosses the boundaries ofexisting disciplines. Many self-assembling systems are rangefrom bi- andtri-block copolymers to DNA structures as well as simple and complex proteins andpeptides. The ultimate goal is to harness molecular self-assembly such that design andcontrol ofbottom-up processes is achieved thereby enabling exploitation of structures developed at the meso- and macro-scopic scale for the purposes oflife and non-life science applications. Such aspirations can be achievedthrough understanding thefundamental principles behind the selforganisation and self-synthesis processes exhibited by biological systems.

Single Amino Acid Based Self-Assemblies of Cysteine and Methionine

2018 ◽  
Author(s):  
Nidhi Gour ◽  
Bharti Koshti ◽  
Chandra Kanth P. ◽  
Dhruvi Shah ◽  
Vivek Shinh Kshatriya ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Self Assembly ◽  
Aromatic Amino Acids ◽  
Neutral Condition ◽  
Single Amino Acid ◽  
Binding Assays ◽  
Human Neuroblastoma ◽  
Cytotoxicity Assays ◽  
First Time

We report for the very first time self-assembly of Cysteine and Methionine to discrenible strucutres under neutral condition. To get insights into the structure formation, thioflavin T and Congo red binding assays were done which revealed that aggregates may not have amyloid like characteristics. The nature of interactions which lead to such self-assemblies was purported by coincubating assemblies in urea and mercaptoethanol. Further interaction of aggregates with short amyloidogenic dipeptide diphenylalanine (FF) was assessed. While cysteine aggregates completely disrupted FF fibres, methionine albeit triggered fibrillation. The cytotoxicity assays of cysteine and methionine structures were performed on Human Neuroblastoma IMR-32 cells which suggested that aggregates are not cytotoxic in nature and thus, may not have amyloid like etiology. The results presented in the manuscript are striking, since to the best of our knowledge,this is the first report which demonstrates that even non-aromatic amino acids (cysteine and methionine) can undergo spontaneous self-assembly to form ordered aggregates.

Probing the Dynamic Self-Assembly Behaviour of Photoswitchable Wormlike Micelles in Real Time

2018 ◽  
Author(s):  
Elaine A. Kelly ◽  
Judith E. Houston ◽  
Rachel Evans
Keyword(s):  
Real Time ◽  
Absorption Spectroscopy ◽  
Self Assembly ◽  
Uv Light ◽  
Wormlike Micelles ◽  
Tetraethylene Glycol ◽  
Light Illumination ◽  
First Time ◽  
Dependent Processes

Understanding the dynamic self-assembly behaviour of azobenzene photosurfactants (AzoPS) is crucial to advance their use in controlled release applications such as<i></i>drug delivery and micellar catalysis. Currently, their behaviour in the equilibrium <i>cis-</i>and <i>trans</i>-photostationary states is more widely understood than during the photoisomerisation process itself. Here, we investigate the time-dependent self-assembly of the different photoisomers of a model neutral AzoPS, <a>tetraethylene glycol mono(4′,4-octyloxy,octyl-azobenzene) </a>(C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>) using small-angle neutron scattering (SANS). We show that the incorporation of <i>in-situ</i>UV-Vis absorption spectroscopy with SANS allows the scattering profile, and hence micelle shape, to be correlated with the extent of photoisomerisation in real-time. It was observed that C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>could switch between wormlike micelles (<i>trans</i>native state) and fractal aggregates (under UV light), with changes in the self-assembled structure arising concurrently with changes in the absorption spectrum. Wormlike micelles could be recovered within 60 seconds of blue light illumination. To the best of our knowledge, this is the first time the degree of AzoPS photoisomerisation has been tracked <i>in</i><i>-situ</i>through combined UV-Vis absorption spectroscopy-SANS measurements. This technique could be widely used to gain mechanistic and kinetic insights into light-dependent processes that are reliant on self-assembly.

Application of Novel Low Current OBIRCH Amplifier and Nanoprobing to Identify Subtle Leakages in Advanced Node Technologies

2018 ◽  
Author(s):  
Satish Kodali ◽  
Liangshan Chen ◽  
Yuting Wei ◽  
Tanya Schaeffer ◽  
Chong Khiam Oh
Keyword(s):  
Signal To Noise Ratio ◽  
Semiconductor Industry ◽  
Fault Isolation ◽  
Ring Oscillator ◽  
High Signal ◽  
The Novel ◽  
Resistance Change ◽  
Three Samples ◽  
Relative Threshold ◽  
First Time

Abstract Optical beam induced resistance change (OBIRCH) is a very well-adapted technique for static fault isolation in the semiconductor industry. Novel low current OBIRCH amplifier is used to facilitate safe test condition requirements for advanced nodes. This paper shows the differences between the earlier and novel generation OBIRCH amplifiers. Ring oscillator high standby leakage samples are analyzed using the novel generation amplifier. High signal to noise ratio at applied low bias and current levels on device under test are shown on various samples. Further, a metric to demonstrate the SNR to device performance is also discussed. OBIRCH analysis is performed on all the three samples for nanoprobing of, and physical characterization on, the leakage. The resulting spots were calibrated and classified. It is noted that the calibration metric can be successfully used for the first time to estimate the relative threshold voltage of individual transistors in advanced process nodes.

Directive Effect of Chain Length in Modulating Peptide Nano-assemblies

2020 ◽  
Vol 27 (9) ◽  
pp. 923-929
Author(s):  
Gaurav Pandey ◽  
Prem Prakash Das ◽  
Vibin Ramakrishnan
Keyword(s):  
Electron Microscopy ◽  
Amino Acids ◽  
Light Scattering ◽  
Chain Length ◽  
Self Assembly ◽  
The Self ◽  
Ionic Charge ◽  
Self Assembling ◽  
Biophysical Techniques

Background: RADA-4 (Ac-RADARADARADARADA-NH2) is the most extensively studied and marketed self-assembling peptide, forming hydrogel, used to create defined threedimensional microenvironments for cell culture applications. Objectives: In this work, we use various biophysical techniques to investigate the length dependency of RADA aggregation and assembly. Methods: We synthesized a series of RADA-N peptides, N ranging from 1 to 4, resulting in four peptides having 4, 8, 12, and 16 amino acids in their sequence. Through a combination of various biophysical methods including thioflavin T fluorescence assay, static right angle light scattering assay, Dynamic Light Scattering (DLS), electron microscopy, CD, and IR spectroscopy, we have examined the role of chain-length on the self-assembly of RADA peptide. Results: Our observations show that the aggregation of ionic, charge-complementary RADA motifcontaining peptides is length-dependent, with N less than 3 are not forming spontaneous selfassemblies. Conclusion: The six biophysical experiments discussed in this paper validate the significance of chain-length on the epitaxial growth of RADA peptide self-assembly.

Rhythmic Prose in Imperial Greek Literature

2018 ◽  
Author(s):  
G. O. Hutchinson
Keyword(s):  
The Novel ◽  
Greek Literature ◽  
System A ◽  
First Time

The chapter looks at the division between poetry and prose in ancient and other literatures, and shows the importance of rhythmic patterning in ancient prose. The development of rhythmic prose in Greek and Latin is sketched, the system explained and illustrated (from Latin). It is firmly established, for the first time, which of the main Greek non-Christian authors 31 BC–AD 300 write rhythmically. The method takes a substantial sample of random sentence-endings (usually 400) from each of a large number of Imperial authors; it compares that sample with one sample of the same size (400) drawn randomly from a range of authors earlier than the invention of this rhythmic system. A particular sort of X2-test is applied. Many Imperial authors, it emerges, write rhythmically; many do not. The genres most likely to offer rhythmic writing are, unexpectedly, narrative: historiography and the novel.

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