Controlling the Biodegradation of Magnesium Implants Through Nanostructured Coatings

2011 ◽  
Author(s):  
Maria E. Iskandar ◽  
Jaclyn Y. Lock ◽  
Arash Aslani ◽  
Huinan Liu
Keyword(s):  
Orthopedic Implants ◽  
Biological Properties ◽  
Culture Conditions ◽  
Medical Implants ◽  
Promising Alternative ◽  
Tissue Integration ◽  
Metallic Biomaterials ◽  
Device Applications ◽  
Phosphate Buffered Saline ◽  
Cell Culture Conditions

Magnesium (Mg) alloys, a novel class of degradable, metallic biomaterials, have attracted growing interest as a promising alternative for medical implant and device applications due to their advantageous mechanical and biological properties. Moreover, magnesium is biodegradable in the physiological environments. The major obstacle for Mg to be used as medical implants is its rapid degradation in physiological fluids. Therefore, the present key challenge lies in controlling Mg degradation rate in the physiological environment. The objective of this study is to develop a nanostructured-hydroxyapatite (nHA) coating on Mg implants to control the degradation and bone tissue integration of the implants. Nanostructured-HA coatings are deposited on magnesium using the Spire’s patented TPA process to moderate the aggressive degradation of magnesium and to improve fast osteointegration between magnesium and natural bone. Morphology and element compositions were characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analysis. The degradation of nHA coated Mg and uncoated Mg was investigated by incubating samples in phosphate buffered saline (PBS) under standard cell culture conditions. The degradation results suggest the nanocoatings positively mediated magnesium degradation. Therefore, nHA coatings are promising for controlling the biodegradation of magnesium-based orthopedic implants and devices.

Lectin Binding Studies on RNA Tumor Virus-Infected Cells

1975 ◽  
Vol 33 ◽  
pp. 326-327
Author(s):  
D. C. Hixson
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Culture Conditions ◽  
3T3 Cells ◽  
Binding Studies ◽  
Con A ◽  
Microscopic Studies ◽  
Tumor Virus ◽  
Infected Cells ◽  
Cell Culture Conditions

The abilities of plant lectins to preferentially agglutinate malignant cells and to bind to specific monosaccharide or oligosaccharide sequences of glycoproteins and glycolipids make them a new and important biochemical probe for investigating alterations in plasma membrane structure which may result from malignant transformation. Electron and light microscopic studies have demonstrated clustered binding sites on surfaces of SV40-infected or tryp- sinized 3T3 cells when labeled with concanavalin A (con A). No clustering of con A binding sites was observed in normal 3T3 cells. It has been proposed that topological rearrangement of lectin binding sites into clusters enables con A to agglutinate SV40-infected or trypsinized 3T3 cells (1). However, observations by other investigators have not been consistent with this proposal (2) perhaps due to differences in reagents used, cell culture conditions, or labeling techniques. The present work was undertaken to study the lectin binding properties of normal and RNA tumor virus-infected cells and their associated viruses using lectins and ferritin-conjugated lectins of five different specificities.

scholarly journals Electrochemical Stability of Poly(3,4-Ethylenedioxythiophene) Derivatives Under Cell Culture Conditions

2021 ◽  
Vol 1885 (3) ◽  
pp. 032004
Author(s):  
Qichao Pan ◽  
Zuwei Zhang ◽  
Yaqiong Zhang ◽  
Yaopeng Zhang ◽  
Bo Zhu
Keyword(s):  
Cell Culture ◽  
Culture Conditions ◽  
Electrochemical Stability ◽  
Cell Culture Conditions

scholarly journals Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves

2021 ◽  
Vol 22 (2) ◽  
pp. 674
Author(s):  
Óscar Darío García-García ◽  
Marwa El Soury ◽  
David González-Quevedo ◽  
David Sánchez-Porras ◽  
Jesús Chato-Astrain ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Nerve Repair ◽  
Wistar Rat ◽  
Biomechanical Properties ◽  
Biological Properties ◽  
Suitable Alternative ◽  
Promising Alternative ◽  
Histological Pattern ◽  
The Impact

Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.

Influence of cell culture conditions on aromatase activity in human genital skin fibroblasts

1989 ◽  
Vol 25 (9) ◽  
pp. 806-812 ◽  
Author(s):  
Tarek Bisat ◽  
Terry R. Brown ◽  
Claude J. Migeon ◽  
Gary D. Berkovitz
Keyword(s):  
Cell Culture ◽  
Culture Conditions ◽  
Skin Fibroblasts ◽  
Aromatase Activity ◽  
Cell Culture Conditions

scholarly journals Glioma cells showing IDH1 mutation cannot be propagated in standard cell culture conditions

2011 ◽  
Vol 104 (6) ◽  
pp. 968-970 ◽  
Author(s):  
S Piaskowski ◽  
M Bienkowski ◽  
E Stoczynska-Fidelus ◽  
R Stawski ◽  
M Sieruta ◽  
...  
Keyword(s):  
Cell Culture ◽  
Glioma Cells ◽  
Culture Conditions ◽  
Idh1 Mutation ◽  
Standard Cell ◽  
Cell Culture Conditions
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