Model-Based Shear Stress Gradient in Realistic Vascular Flows and Its Relation to Arterial Macromolecular Permeability

2003 ◽  
Author(s):  
Jeffrey A. LaMack ◽  
Heather A. Himburg ◽  
Xue-Mei Li ◽  
Morton H. Friedman
Keyword(s):  
Shear Stress ◽  
Fluid Dynamic ◽  
Stress Gradient ◽  
Blue Dye ◽  
Infrarenal Aorta ◽  
Flow Measurements ◽  
Average Shear Stress ◽  
Average Shear ◽  
Time Average ◽  
Shear Stress Gradient

Evans blue dye (EBD) was injected into the carotid arteries of three anesthetized pigs and allowed to circulate for 90 minutes. At the conclusion of the 90-minute period, the animals were sacrificed and injection casts of the infrarenal aorta and iliac-femoral arteries were prepared. The casts with their surrounding arteries were removed and immersed in fixative. After fixation, the EBD-stained vessels were separated from the casts, which were used to construct computational meshes for simulation of the flow fields and wall shear stress distributions that had existed in the casted regions during the experiments. The inlet flow waves and flow partitions were based on flow measurements performed during each experiment. Based on a conceptual model of the relation between shear stress nonuniformity and permeability increase, the spatial and angular variation of the gradient of the time-average shear stress at the walls of the external iliac arteries was found from the computational fluid dynamic simulations for each experiment. Using affine transformations, the gradient and time-average shear stress results, and the EBD optical density distributions, were mapped to a common template, allowing pixel-by-pixel correlations of the hemodynamic stress parameters and local permeability. The results suggest that both shear stress gradient and time-average shear play a role in determining vascular permeability to macromolecules.

scholarly journals Endothelial cell responses to atheroprone flow are driven by two separate flow components: low time-average shear stress and fluid flow reversal

2010 ◽  
Vol 298 (2) ◽  
pp. H367-H374 ◽  
Author(s):  
Daniel E. Conway ◽  
Marcie R. Williams ◽  
Suzanne G. Eskin ◽  
Larry V. McIntire
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Umbilical Vein ◽  
Steady Shear ◽  
Monocyte Adhesion ◽  
Average Shear Stress ◽  
Average Shear ◽  
Time Average

To simulate the effects of shear stress in regions of the vasculature prone to developing atherosclerosis, we subjected human umbilical vein endothelial cells to reversing shear stress to mimic the hemodynamic conditions at the wall of the carotid sinus, a site of complex, reversing blood flow and commonly observed atherosclerosis. We compared the effects of reversing shear stress (time-average: 1 dyn/cm2, maximum: +11 dyn/cm2, minimum: −11 dyn/cm2, 1 Hz), arterial steady shear stress (15 dyn/cm2), and low steady shear stress (1 dyn/cm2) on gene expression, cell proliferation, and monocyte adhesiveness. Microarray analysis revealed that most differentially expressed genes were similarly regulated by all three shear stress regimens compared with static culture. Comparisons of the three shear stress regimens to each other identified 138 genes regulated by low average shear stress and 22 genes regulated by fluid reversal. Low average shear stress induced increased cell proliferation compared with high shear stress. Only reversing shear stress exposure induced monocyte adhesion. The adhesion of monocytes was partially inhibited by the incubation of endothelial cells with ICAM-1 blocking antibody. Increased heparan sulfate proteoglycan expression was observed on the surface of cells exposed to reversing shear stress. Heparinase III treatment significantly reduced monocyte adhesion. Our results suggest that low steady shear stress is the major impetus for differential gene expression and cell proliferation, whereas reversing flow regulates monocyte adhesion.

WALL SHEAR STRESS GRADIENT ANALYSIS WITHIN AN IDEALIZED STENOSIS USING NON-NEWTONIAN FLOW

Neurosurgery ◽  
2007 ◽  
Vol 61 (4) ◽  
pp. 853-864 ◽  
Author(s):  
Clemens M. Schirmer ◽  
Adel M. Malek
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Fluid Dynamic ◽  
Stress Gradient ◽  
Focal Region ◽  
Symptomatic Carotid ◽  
Wall Shear Stress Gradient ◽  
Shear Stress Gradient ◽  
Wall Shear ◽  
Near Wall

Abstract OBJECTIVE The endothelium is functionally regulated by the magnitude and spatiotemporal gradients of wall shear stress (WSS). Although flow separation and reversal occur beyond high-grade stenoses, little is known of the WSS pattern within clinically relevant mild to moderate stenoses. METHODS An axisymmetric geometry with 25, 50, and 75% stenosis criteria (quantified in accordance with the North American Symptomatic Carotid Endarterectomy Trial) was used to generate a high-resolution, hybrid, tetrahedral-hexahedral computational mesh with boundary-layer enrichment to improve near-wall shear stress gradient (WSSG) computation. Time-dependent computational fluid dynamic analysis was performed using a non-Newtonian Carreau-Yasuda model of blood to yield the shear-dependent viscosity. RESULTS Transition to secondary flow patterns was demonstrated in stenoses of 25, 50, and 75%. A focal region with near-wall flow reversal and retrograde WSS was identified within the stenosis itself and was found to migrate cyclically during the cardiac pulse. A zone of zero WSS and divergent WSSG that shifts in toward the throat with increasing stenotic severity was identified. Focal zones of high WSSG with converging and/or diverging direction were uncovered within the stenosis itself, as were expected changes in the distal poststenotic region. These zones of divergent WSSG shift over a substantial length of the stenosis during the course of the cardiac cycle. CONCLUSION Luminal WSS demonstrates dynamic direction reversal and high spatial gradients within the distal stenosis throat of even clinically moderate lesions. These findings shed light on the complex vessel wall hemodynamics within clinical stenoses and reveal a mechanical microenvironment that is conducive to perpetual endothelial functional dysregulation and stenosis progression.

scholarly journals Numerical Study of Fluid Dynamics in Suspension Culture of iPS Cells

2019 ◽  
Vol 17 (1) ◽  
pp. 73 ◽  
Author(s):  
Masaki Yano ◽  
Takuya Yamamoto ◽  
Yasunori Okano ◽  
Toshiyuki Kanamori ◽  
Mashiro Kino–oka
Keyword(s):  
Shear Stress ◽  
Suspension Culture ◽  
Numerical Study ◽  
Maximum Shear Stress ◽  
Ips Cells ◽  
Stirred Tanks ◽  
Average Shear Stress ◽  
Average Shear ◽  
Orbital Shaking ◽  
Induced Pluripotent

In a suspension culture of iPS cells, the shear stress generated during mixing is expected to promote differentiation of induced pluripotent stem (iPS) cells. The stress on the cells can be controlled by rotational rate and shape of impeller. However, it is difficult to optimize these operative parameters by experiments. Therefore, we have developed a numerical model to obtain the average and the maximum shear stress in two kinds of stirred tanks and an orbital shaking cylindrical container. The present results showed that the shear stress strongly depended on the type of mixing and lesser extent on the shape of the impeller. The average shear stress is larger in the shaking mode than that in the stirring mode. In contrast, the maximum shear stress is much smaller in the shaking than the stirring. These results suggest that stirring and shaking should be selectively used depending on the application

scholarly journals Effects of Nanoparticle Enhanced Lubricant Films in Thermal Design of Plain Journal Bearings at High Reynolds Numbers

Symmetry ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1353 ◽  
Author(s):  
Abdollahzadeh Jamalabadi ◽  
Alamian ◽  
Yan ◽  
Li ◽  
Leveneur ◽  
...  
Keyword(s):  
Heat Transfer ◽  
Shear Stress ◽  
Temperature Rise ◽  
Rotational Speed ◽  
Volume Fraction ◽  
Journal Bearings ◽  
Thermal Design ◽  
Average Shear Stress ◽  
Average Shear ◽  
Dissipation Power

Performance investigation of oil journal bearings is of particular importance given the growing use of them as a support for rotary components in a wide range of industrial machines. Frictional forces and shear stresses, which are proportionate to the velocity of lubricating layers at different points in the bearing space, provide the basis for changing temperature conditions. Various factors such as rotational velocity increase, slip width reduction, and small heat transfer coefficient of lubricant cause intensification of lubricant temperature changes. In the present study, with using computational fluid dynamic (CFD) thermohydrodynamic (THD) numerical simulations, the effect of nanoparticles on the performance features of plain journal bearings is evaluated. Particularly, 3D simulation of a journal bearing is implemented using CFD which considerably improves the accuracy of results, coupled with conjugate heat transfer model for metal parts of bearings. Reynolds equation model is used to calculate the oil-film pressure developed in hydrodynamic journal bearings by applying the nano-based lubricants. The configuration of thrust bearing consists of six pads in this study. In order to reduce the modeling complexity and computational cost and because of the symmetrical geometry of the pads, simulation of a single pad is considered instead of the entire domain. In this study, TiO2 nanoparticle with different volume fraction percentages are used. The parameters that are changed to evaluate the performance of the bearing include volume fraction percentage of the nanoparticle, type of lubricant, and rotational speed. Based on the results, for all different lubricant types, the dissipation power, average shear stress, and temperature rise are increased with augmenting the rotational speed. By increasing the rotational speed from 500 to 1500 rpm, the average shear stress increases by more than 100%, 120%, and 130% for DTE 26, DTE 25, and DTE 24 lubricant types, respectively. Moreover, by increasing the rotational speed from 500 to 1500 rpm, the dissipation power, and temperature rise are increased around 600% and 800%, respectively. Furthermore, increasing nanoparticles volume fraction from 0% to 10%, increases all parameters by approximately 10% for all lubricant types and in all rotational speeds.

Numerical Investigation and Prediction of Atherogenic Sites in Branching Arteries

1995 ◽  
Vol 117 (3) ◽  
pp. 350-357 ◽  
Author(s):  
M. Lei ◽  
C. Kleinstreuer ◽  
G. A. Truskey
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Blood Vessels ◽  
Vector Fields ◽  
Stress Gradient ◽  
Western World ◽  
Medium Size ◽  
Wall Shear Stress Gradient ◽  
Shear Stress Gradient ◽  
Wall Shear

Atherosclerosis, a disease of large- and medium-size arteries, is the chief cause of death in the US and most of the western world. It is widely accepted that the focal nature of the disease in arterial bends, junctions, and bifurcations is directly related to locally abnormal hemodynamics, often labeled “disturbed flows.” Employing the aorto-celiac junction of rabbits as a representative atherosclerotic model and considering other branching blood vessels with their distinctive input wave forms, it is suggested that the local wall shear stress gradient (WSSG) is the single best indicator of nonuniform flow fields leading to atherogenesis. Alternative predictors of susceptible sites are briefly evaluated. The results discussed include transient velocity vector fields, wall shear stress gradient distributions, and a new dimensionless parameter for the prediction of the probable sites of stenotic developments in branching blood vessels. Some of the possible underlying biological aspects of atherogenesis due to locally significant |WSSG|-magnitudes are briefly discussed.

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