In-Vitro Diagnosis of Colon Cancer Using Bio-Functionalized Magnetic Nanoparticles

2011 ◽  
Author(s):  
Chin-Yih Hong ◽  
Shieh-Yueh Yang ◽  
K. W. Huang ◽  
Herng-Er Horng ◽  
Hong-Chang Yang
Keyword(s):  
Colon Cancer ◽  
Magnetic Nanoparticles ◽  
Detection Limit ◽  
Dynamic Range ◽  
Risk Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Detection Range ◽  
Low Detection Limit ◽  
In Vitro Diagnosis

The popular bio-marker for colon cancer is carcinoembryonic antigen (CEA). By conjugating anti-CEA onto magnetic nanoparticles, CEA can be specially labeled and detected by measuring magnetic signals via immunomagnetic reduction (IMR). The low detection limit and detection range of IMR on CEA are investigated. The results are compared with those by using the existing assay, such as enzyme-linked immunosorbent assay (ELISA). It is evidenced that IMR has sensitivity much higher than that of ELISA. The low detection limit is below the normal level of CEA concentration of clinic practice and is suitable for early-stage in-vitro diagnosis for colon cancer. Furthermore, the dynamic range of detection for the CEA concentration using IMR extends well above the threshold of high risk level of colorectal carcinoma in current diagnosing practice. Therefore, IMR is also suitable in other stages diagnosis for colon cancer development.

Schisandrin B Attenuates Colitis-Associated Colorectal Cancer through SIRT1 Linked SMURF2 Signaling

2021 ◽  
pp. 1-17
Author(s):  
Zhichen Pu ◽  
Weiwei Zhang ◽  
Minhui Wang ◽  
Maodi Xu ◽  
Haitang Xie ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Cell Growth ◽  
Protein Expression ◽  
Hct116 Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Schisandrin B ◽  
In Vivo Models

Colon cancer, a common type of malignant tumor, seriously endangers human health. However, due to the relatively slow progress in diagnosis and treatment, the clinical therapeutic technology of colon cancer has not been substantially improved in the past three decades. The present study was designed to investigate the effects and involved mechanisms of schisandrin B in cell growth and metastasis of colon cancer. C57BL/6 mice received AOM and dextran sulfate sodium. Mice in treatment groups were gavaged with 3.75–30 mg/kg/day of schisandrin B. Transwell chamber migration, enzyme-linked immunosorbent assay (ELISA), Western blot analysis, immunoprecipitation (IP) and immunofluorescence were conducted, and HCT116 cell line was employed in this study. Data showed that schisandrin B inhibited tumor number and tumor size in the AOD+DSS-induced colon cancer mouse model. Schisandrin B also inhibited cell proliferation and metastasis of colon cancer cells. We observed that schisandrin B induced SMURF2 protein expression and affected SIRT1 in vitro and in vivo. SMURF2 interacted with SIRT1 protein, and there was a negative correlation between SIRT1 and SMURF2 expressions in human colorectal cancer. The regulation of SMURF2 was involved in the anticancer effects of schisandrin B in both in vitro and in vivo models. In conclusion, the present study revealed that schisandrin B suppressed SIRT1 protein expression, and SIRT1 is negatively correlated with the induction of SMURF2, which inhibited cell growth and metastasis of colon cancer. Schisandrin B could be a leading compound, which will contribute to finding novel potential agents and therapeutic targets for colon cancer.

scholarly journals Nanostructured copper selenide as an ultrasensitive and selective non-enzymatic glucose sensor

2021 ◽  
Author(s):  
Siddesh Umapathi ◽  
Harish Singh ◽  
Jahangir Masud ◽  
Manashi Nath
Keyword(s):  
Detection Limit ◽  
High Efficiency ◽  
Glucose Sensor ◽  
High Sensitivity ◽  
Copper Selenide ◽  
Glucose Detection ◽  
Applied Potential ◽  
Detection Range ◽  
Low Detection Limit ◽  
Linear Detection

CuSe nanostructures exhibit high-efficiency for glucose detection with high sensitivity (19.419 mA mM−1 cm−2) and selectivity at low applied potential (0.15 V vs. Ag|AgCl), low detection limit (0.196 μM) and linear detection range (100 nM to 40 μM).

A nanofluidic device for ultrasensitive and label-free detection of tetracycline in association with γ-cyclodextrin and GO

2021 ◽  
Vol 13 (15) ◽  
pp. 1832-1838
Author(s):  
Jiaxi Cheng ◽  
Fenghua Jiang ◽  
Siqi Zhang
Keyword(s):  
Detection Limit ◽  
Water Samples ◽  
Recovery Rate ◽  
Label Free ◽  
Detection Range ◽  
Low Detection Limit ◽  
Sensing Platform ◽  
Label Free Detection ◽  
Nanofluidic Device

We develop a label-free, sensitive and selective nanochannel sensing platform for detection of TC. The nanosensor provided a low detection limit, a wide detection range and excellent recovery rate in different water samples.

scholarly journals Single-Atom Cobalt-Based Electrochemical Biomimetic Uric Acid Sensor with Wide Linear Range and Ultralow Detection Limit

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Fang Xin Hu ◽  
Tao Hu ◽  
Shihong Chen ◽  
Dongping Wang ◽  
Qianghai Rao ◽  
...  
Keyword(s):  
Uric Acid ◽  
Detection Limit ◽  
Single Atom ◽  
Practical Application ◽  
Oxidation Of Co ◽  
Renal Disorder ◽  
Detection Range ◽  
Low Detection Limit ◽  
Doped Graphene ◽  
Better Than

Abstract Uric acid (UA) detection is essential in diagnosis of arthritis, preeclampsia, renal disorder, and cardiovascular diseases, but it is very challenging to realize the required wide detection range and low detection limit. We present here a single-atom catalyst consisting of Co(II) atoms coordinated by an average of 3.4 N atoms on an N-doped graphene matrix (A–Co–NG) to build an electrochemical biomimetic sensor for UA detection. The A–Co–NG sensor achieves a wide detection range over 0.4–41,950 μM and an extremely low detection limit of 33.3 ± 0.024 nM, which are much better than previously reported sensors based on various nanostructured materials. Besides, the A–Co–NG sensor also demonstrates its accurate serum diagnosis for UA for its practical application. Combination of experimental and theoretical calculation discovers that the catalytic process of the A–Co–NG toward UA starts from the oxidation of Co species to form a Co3+–OH–UA*, followed by the generation of Co3+–OH + *UA_H, eventually leading to N–H bond dissociation for the formation of oxidized UA molecule and reduction of oxidized Co3+ to Co2+ for the regenerated A–Co–NG. This work provides a promising material to realize UA detection with wide detection range and low detection limit to meet the practical diagnosis requirements, and the proposed sensing mechanism sheds light on fundamental insights for guiding exploration of other biosensing processes.

Non-enzymatic amperometric sensing of glucose by employing sucrose templated microspheres of copper oxide (CuO)

2016 ◽  
Vol 45 (13) ◽  
pp. 5833-5840 ◽  
Author(s):  
Mohit Saraf ◽  
Kaushik Natarajan ◽  
Shaikh M. Mobin
Keyword(s):  
Detection Limit ◽  
Copper Oxide ◽  
Glucose Sensor ◽  
Hydrothermal Reaction ◽  
Copper Nitrate ◽  
Good Sensitivity ◽  
Detection Range ◽  
Low Detection Limit ◽  
Linear Detection ◽  
Amperometric Sensing

An enzymeless glucose sensor (MCSPE) based on copper oxide microspheres (CMS) prepared by hydrothermal reaction of copper nitrate and sucrose, can sense glucose in a wide linear detection range with good sensitivity and low detection limit.

scholarly journals Lanthanide-Doped Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Carbohydrate Antigen 19-9

2021 ◽  
Vol 8 ◽  
Author(s):  
Chaohui Zhou ◽  
Zhongyun Chu ◽  
Wenyue Hou ◽  
Xiuying Wang
Keyword(s):  
Immunosorbent Assay ◽  
Dynamic Range ◽  
Carbohydrate Antigen ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Upconversion Nanoparticles ◽  
Fluorescence Signal ◽  
Signal Transducer ◽  
Detection Range

Lanthanide-doped upconversion nanoparticles (UCNPs) have attracted considerable attention in detection of biological analytes and bioimaging owing to their superior optical properties, including high photochemical stability, sharp emission bandwidth, large anti-Stokes shifts, and low toxicity. In this work, we fabricated UCNP-linked immunosorbent assay (ULISA) for the sensitive detection of carbohydrate antigen 19-9 (CA19-9). The design is based on amino-functionalized SiO2-coated Gd-doped NaYF4:Yb3+,Er3+ upconversion nanoparticles (UCNPs@SiO2-NH2) as a direct background-free luminescent reporter; a secondary anti-IgG antibody (Ab2) was conjugated to the surface of UCNPs@SiO2-NH2 (UCNP-Ab2), and UCNP-Ab2 was used for specific targeting of CA19-9. The UCNPs were well characterized by TEM, SEM, XRD, FT-IR, and UV-vis. The detection process was similar to enzyme-linked immunosorbent assay (ELISA). UCNPs were used as signal transducer to replace the color compounds for an enzyme-mediated signal amplification step. An anti-CA19-9 primary antibody (Ab1) was fixed for capturing the CA19-9, and the fluorescence signal was obtained from the specific immunoreaction between UCNP-Ab2 and CA19-9. Under optimum conditions, this ULISA shows sensitive detection of CA19-9 with a dynamic range of 5–2,000 U/ml. The ULISA system shows higher detection sensitivity and wider detection range compared with the traditional ELISA for CA19-9 detection. This strategy using UCNPs as signal transducer may pave a new avenue for the exploration of rare doped UCNPs in ELISA assay for clinical applications in the future.

scholarly journals Rapid and Sensitive Inhibitor Screening Using Magnetically Modulated Biosensors

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4814
Author(s):  
Shira Roth ◽  
Amos Danielli
Keyword(s):  
Neutralizing Antibodies ◽  
Screening Tool ◽  
Dynamic Range ◽  
Specific Binding ◽  
Enzyme Linked Immunosorbent Assay ◽  
Angiotensin Converting Enzyme 2 ◽  
Inhibitor Screening ◽  
Drug Candidates ◽  
Potential Inhibitors

Inhibitor screening is an important tool for drug development, especially during the COVID-19 pandemic. The most used in vitro inhibitor screening tool is an enzyme-linked immunosorbent assay (ELISA). However, ELISA-based inhibitor screening is time consuming and has a limited dynamic range. Using fluorescently and magnetically modulated biosensors (MMB), we developed a rapid and sensitive inhibitor screening tool. This study demonstrates its performance by screening small molecules and neutralizing antibodies as potential inhibitors of the interaction between the spike protein 1 (S1) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the angiotensin-converting enzyme 2 (ACE2) receptor. The MMB-based assay is highly sensitive, has minimal non-specific binding, and is much faster than the commonly used ELISA (2 h vs. 7–24 h). We anticipate that our method will lead to a remarkable advance in screening for new drug candidates.

Optical fiber refractive index sensor with low detection limit and large dynamic range using a hybrid fiber interferometer

2019 ◽  
pp. 1-1 ◽  
Author(s):  
Xuegang Li ◽  
Stephen Christopher Warren-Smith ◽  
Heike Ebendorff-Heidepriem ◽  
Ya-nan Zhang ◽  
Linh Viet Nguyen
Keyword(s):  
Refractive Index ◽  
Detection Limit ◽  
Optical Fiber ◽  
Dynamic Range ◽  
Refractive Index Sensor ◽  
Large Dynamic Range ◽  
Hybrid Fiber ◽  
Low Detection Limit

scholarly journals Miscanthus grass-derived carbon dots to selectively detect Fe3+ ions

RSC Advances ◽  
2019 ◽  
Vol 9 (15) ◽  
pp. 8628-8637 ◽  
Author(s):  
Maisyn Picard ◽  
Suman Thakur ◽  
Manjusri Misra ◽  
Amar K. Mohanty
Keyword(s):  
Detection Limit ◽  
Carbon Dots ◽  
Sensitive Detection ◽  
Detection Range ◽  
Low Detection Limit ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fluorescent carbon dots for the selective and sensitive detection of Fe3+ ions with a wide detection range and very low detection limit.

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