Design of Winding With Two Rovings for Cost Efficiency and Quality in Robotized Filament Winding

2003 ◽  
Author(s):  
W. Polini ◽  
L. Sorrentino
Keyword(s):  
Complex Shape ◽  
Industrial Robot ◽  
Experimental Tests ◽  
Filament Winding ◽  
Part Quality ◽  
Integrated Environment ◽  
A Cell ◽  
Guide System ◽  
Critical Process Parameters ◽  
Pass Through

This work deals with the design of winding with two rovings of asymmetric and complex shape parts by means of robotized filament winding technology. The adopted criteria aim to decrease significantly the winding time and, therefore, the manufacturing costs by preserving the composite part quality through the design of a system that is able to control the orientation of both the rovings on the die during winding, thus avoiding their torsion. A cell composed of an industrial robot opportunely equipped with a new feed and deposition system has been designed and implemented. Two rovings are unwound by two spools fixed to a creel and, then, they are conveyed towards the new feed and deposition system connected to the robot end-effector. The rovings pass through the roving-guide system, where they are paired off in a single roving that is guided towards the deposition head. The deposition head controls the orientation of the single roving on the die during winding. The new system working and its interaction with the most critical process parameters have been evaluated through experimental tests. This work belongs to a research project aiming to develop an integrated environment to wind complex shape parts through a robotized cell.

Design of a New Feed-Deposition Head for Robotized Filament Winding

2002 ◽  
Author(s):  
L. Carrino ◽  
W. Polini ◽  
L. Sorrentino
Keyword(s):  
Process Parameters ◽  
Complex Shape ◽  
Experimental Tests ◽  
Filament Winding ◽  
Part Quality ◽  
Integrated Environment ◽  
Guide System ◽  
Critical Process Parameters ◽  
Critical Process ◽  
High Repeatability

This work deals with the design of a new feed and deposition head for a robotized cell able to manufacture complex shape parts in composite material by means of the filament winding technology. The adopted criteria aim to increase both the filament winding efficiency and the composite part quality by designing a system able to control the process parameters, such as the roving tension, the winding speed and the winding trajectory through a very compact and flexible frame, that presents in a single solution the pinboard, the roving tensioner, the roving-guide system and the deposition head. Moreover, the same frame is able to satisfy manufacturing and assembling requirements. Finally, the new proposed system has been designed in such a way as to be easily adaptable to every robot or machine used to wind, since it has a flange that may be easily connected with the robot end-effector by warranting a high precision and a high repeatability of the roving locating during the winding process. The new head working and its interaction with the most critical process parameters have been evaluated through experimental tests. This work belongs to a research project aiming to develop an integrated environment for winding complex shape parts through a robotized cell.

Design of Deposition Head Trajectory for Robotized Filament Winding of Complex Shape Parts

2004 ◽  
Author(s):  
W. Polini ◽  
L. Sorrentino
Keyword(s):  
Mechanical Properties ◽  
Composite Material ◽  
Complex Shape ◽  
Experimental Tests ◽  
Filament Winding ◽  
Structural Constraints ◽  
Cad Cam ◽  
Cam Software ◽  
Wound Composite

When the roving is winding on the die, the tension value may move away the nominal one that has been optimized by considering the quality and the mechanical properties of the wound composite parts. The variance of the tension value during winding from the nominal one strongly depends on the deposition head trajectory. The present work focuses on the planning of the winding trajectory for winding complex shape parts in composite material by a robotized cell. The planning of the winding trajectory should be based on the structural constraints of the robotized cell and on the technological requirements of the process. In particular, this work aims to study the conditions by which the value of the roving tension verges on the nominal one during winding. The developed planning logics and implemented by a CAD/CAM software have been validated by experimental tests. This work represents the first step towards the optimization of the winding trajectory.

scholarly journals Human–Robot Interaction through Eye Tracking for Artistic Drawing

Robotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 54
Author(s):  
Lorenzo Scalera ◽  
Stefano Seriani ◽  
Paolo Gallina ◽  
Mattia Lentini ◽  
Alessandro Gasparetto
Keyword(s):  
Eye Tracking ◽  
Assistive Technology ◽  
Movement Disorders ◽  
Industrial Robot ◽  
Experimental Tests ◽  
Human Robot Interaction ◽  
Eye Tracker ◽  
Robot Interaction ◽  
Eye Motion ◽  
Artistic Images

In this paper, authors present a novel architecture for controlling an industrial robot via an eye tracking interface for artistic purposes. Humans and robots interact thanks to an acquisition system based on an eye tracker device that allows the user to control the motion of a robotic manipulator with his gaze. The feasibility of the robotic system is evaluated with experimental tests in which the robot is teleoperated to draw artistic images. The tool can be used by artists to investigate novel forms of art and by amputees or people with movement disorders or muscular paralysis, as an assistive technology for artistic drawing and painting, since, in these cases, eye motion is usually preserved.

Effect of PCMBS on CO2permeability of Xenopus oocytes expressing aquaporin 1 or its C189S mutant

1998 ◽  
Vol 275 (6) ◽  
pp. C1481-C1486 ◽  
Author(s):  
Gordon J. Cooper ◽  
Walter F. Boron
Keyword(s):  
Carbonic Anhydrase ◽  
Intracellular Ph ◽  
Xenopus Oocytes ◽  
Membrane Lipid ◽  
Wild Type ◽  
Aquaporin 1 ◽  
Gas Channel ◽  
A Cell ◽  
Pass Through ◽  
Rule Out

A recent study on Xenopus oocytes [N. L. Nakhoul, M. F. Romero, B. A. Davis, and W. F. Boron. Am. J. Physiol. 274 ( Cell Physiol. 43): C543–548, 1998] injected with carbonic anhydrase showed that expressing aquaporin 1 (AQP1) increases by ∼40% the rate at which exposing the cell to CO2 causes intracellular pH to fall. This observation is consistent with several interpretations. Overexpressing AQP1 might increase apparent CO2 permeability by 1) allowing CO2 to pass through AQP1, 2) stimulating injected carbonic anhydrase, 3) enhancing the CO2 solubility of the membrane’s lipid, or 4) increasing the expression of a native “gas channel.” The purpose of the present study was to distinguish among these possibilities. We found that expressing the H2O channel AQP1 in Xenopus oocytes increases the CO2 permeability of oocytes in an expression-dependent fashion, whereas expressing the K+ channel ROMK1 has no effect. The mercury derivative p-chloromercuriphenylsulfonic acid (PCMBS), which inhibits the H2O movement through AQP1, also blocks the AQP1-dependent increase in CO2 permeability. The mercury-insensitive C189S mutant of AQP1 increases the CO2 permeability of the oocyte to the same extent as does the wild-type channel. However, the C189S-dependent increase in CO2permeability is unaffected by treatment with PCMBS. These data rule out options 2–4 listed above. Thus our results suggest that CO2passes through the pore of AQP1 and are the first data to demonstrate that a gas can enter a cell by a means other than diffusing through the membrane lipid.

Automation of Chamfering by an Industrial Robot; Development of a System with Reference to Tool Application Direction

2001 ◽  
Vol 13 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Naoki Asakawa ◽  
◽  
Yoshio Mizumoto ◽  
Yoshimi Takeuchi ◽  
Keyword(s):  
Complex Shape ◽  
Tool Path ◽  
Industrial Robot ◽  
Force Sensor ◽  
Experimental Results ◽  
Curved Surface ◽  
Linear Actuators ◽  
Articulated Robot

The study deals with the automatic chamfering for a workpiece with complex shape on the basis of CAD data, using an industrial robot. Tool path, pushing direction and force at the each chamfering point are calculated from CAD data both to keep chamfering conditions stable and to keep a larger range in space by use of a developed system, named TADRPECS, for workpieces with complex shape. As a chamfering tool, a rotary-bar type tool consisting of a force sensor and two linear actuators are mounted to the arm of an articulated robot. From experimental results, the system is found to be effective to chamfer edges of a hole on a workpiece with free curved surface.

S-phase-specific expression of the Mad3 gene in proliferating and differentiating cells

2001 ◽  
Vol 359 (2) ◽  
pp. 361-367 ◽  
Author(s):  
Elizabeth J. FOX ◽  
Stephanie C. WRIGHT
Keyword(s):  
Cell Cycle ◽  
Cellular Proliferation ◽  
Cultured Cells ◽  
S Phase ◽  
Proliferating Cells ◽  
Specific Expression ◽  
Related Function ◽  
Erythroleukemia Cells ◽  
A Cell ◽  
Pass Through

The Myc/Max/Mad transcription factor network plays a central role in the control of cellular proliferation, differentiation and apoptosis. In order to elucidate the biological function of Mad3, we have analysed the precise temporal patterns of Mad3 mRNA expression during the cell cycle and differentiation in cultured cells. We show that Mad3 is induced at the G1/S transition in proliferating cells; expression persists throughout S-phase, and then declines as cells pass through G2 and mitosis. The expression pattern of Mad3 is coincident with that of Cdc2 throughout the cell cycle. In contrast, the expression of Mad3 during differentiation of cultured mouse erythroleukemia cells shows two transient peaks of induction. The first of these occurs at the onset of differentiation, and does not correlate with the S-phase of the cell cycle, whereas the second is coincident with the S-phase burst that precedes the terminal stages of differentiation. Our results therefore suggest that Mad3 serves a cell-cycle-related function in both proliferating and differentiating cells, and that it may also have a distinct role at various stages of differentiation.

Characteristics of a novel, anaerobic, mycoplasma-like bacterium from Ace Lake, Antarctica

1992 ◽  
Vol 4 (2) ◽  
pp. 155-162 ◽  
Author(s):  
P.D. Franzmann ◽  
M. Rohde
Keyword(s):  
Anaerobic Bacterium ◽  
Anaerobic Bacteria ◽  
Taxonomic Status ◽  
Optimum Temperature ◽  
Obligately Anaerobic ◽  
A Cell ◽  
Large Genome Size ◽  
High Concentrations ◽  
Pass Through

An obligately anaerobic bacterium that lacked a cell wall was isolated from the hypolimnion of Ace Lake, Antarctica. Cells were very pleomorphic, forming cocci, filaments up to 25 μm in length, and annular shapes. The organism was morphologically very similar to some members of the class Mollicutes which contains two genera of obligately anaerobic bacteria, Anaeroplasma and Asteroleplasma. Like members of the class, the isolate was resistant to high concentrations of penicillin (1000 Units ml-1). Similar to Anaeroplasma, the organism had a low DNA G+C content (29.3±0.4) and produced hydrogen, carbon dioxide, acetic acid, lactic acid and succinic acid from the fermentation of glucose. However, the taxonomic status of the strain remained unclear as, unlike members of the class Mollicutes, the isolate had a relatively large genome size (2.26±0.11 × 109 daltons), did not pass through 0.45 μm pore size filters, and did not form typical mycoplasma-like colonies. The organism was psychrophilic with an optimum temperature for growth between 12°C and 13°C. A phenotypic description of the organism is given and the ecological role of the organism is inferred from its phenotype and the characteristics of its Antarctic habitat.

Experimental Setup for Harmonic Drive Efficiency Determination

2012 ◽  
Vol 463-464 ◽  
pp. 1518-1521
Author(s):  
Ciprian Ion Rizescu ◽  
Constantin Udrea ◽  
Dana Rizescu
Keyword(s):  
Manufacturing Process ◽  
Industrial Robot ◽  
Experimental Tests ◽  
Experimental Setup ◽  
Harmonic Drive ◽  
Industrial Manufacturing ◽  
Efficiency Determination ◽  
Good Agreement

This paper deals with a harmonic drive, which can be used for an industrial robot, such as RIP 63, COMAU, or KUKA (RRR). The robot could be integrated in an industrial manufacturing process where it has to manipulate / transport bags, sacks, or other loads. The paper presents also the experimental setup for efficiency determination as well as experimental determinations. The computation operations were performed in MATLAB environment and the results were in good agreement with experimental tests.

scholarly journals Centrosome Clustering in the Development of Bovine Binucleate Trophoblast Giant Cells

2016 ◽  
Vol 203 (5) ◽  
pp. 287-294 ◽  
Author(s):  
Karl Klisch ◽  
Elisabeth M. Schraner ◽  
Alois Boos
Keyword(s):  
Cell Cycle ◽  
Tumor Cells ◽  
Molecular Mechanisms ◽  
Giant Cells ◽  
Cell Nuclei ◽  
Pericentriolar Material ◽  
A Cell ◽  
Pass Through ◽  
Diploid Cells ◽  
Trophoblast Giant Cells

Binucleate trophoblast giant cells (BNC) are the characteristic feature of the ruminant placenta. During their development, BNC pass through 2 acytokinetic mitoses and become binucleate with 2 tetraploid nuclei. In this study, we investigate the number and location of centrosomes in bovine BNC. Centrosomes typically consist of 2 centrioles surrounded by electron-dense pericentriolar material. Duplication of centrosomes is tightly linked to the cell cycle, which ensures that the number of centrosomes remains constant in proliferating diploid cells. Alterations of the cell cycle, which affect the number of chromosome sets, also affect the number of centrosomes. In this study, we use placentomal tissue from pregnant cows (gestational days 80-230) for immunohistochemical staining of γ-tubulin (n = 3) and transmission electron microscopy (n = 3). We show that mature BNC have 4 centrosomes with 8 centrioles, clustered in the angle between the 2 cell nuclei. During the second acytokinetic mitosis, the centrosomes must be clustered to form the poles of a bipolar spindle. In rare cases, centrosome clustering fails and tripolar mitosis leads to the formation of trinucleate “BNC”. Generally, centrosome clustering occurs in polyploid tumor cells, which have an increased number of centrioles, but it is absent in proliferating diploid cells. Thus, inhibition of centrosome clustering in tumor cells is a novel promising strategy for cancer treatment. BNC are a cell population in which centrosome clustering occurs as part of the normal life history. Thus, they might be a good model for the study of the molecular mechanisms of centrosome clustering.

A cell for X-ray diffraction in liquids under moderate hydrostatic pressure

1989 ◽  
Vol 22 (6) ◽  
pp. 572-577 ◽  
Author(s):  
M. Geeves ◽  
P. Knight
Keyword(s):  
Hydrostatic Pressure ◽  
High Performance ◽  
X Rays ◽  
X Ray Diffraction ◽  
Plastic Sheet ◽  
X Ray ◽  
Oil Pump ◽  
Sample Chamber ◽  
A Cell ◽  
Pass Through

A portable cell is described which allows X-ray diffraction data to be collected from suspensions or mounted specimens in liquid under hydrostatic pressure. Pressure is applied via either an oil pump or a high-performance liquid chromatography pump. The temperature of the cell is controlled by a thermostatted baseplate. X-rays pass through the stainless steel block via windows of Kapton V plastic sheet which will withstand a pressure difference of at least 15 MPa. Distortion of the plastic under pressure increases the liquid path of the X-rays. This distortion can be reduced by stretching over dome-shaped supports. Under these conditions 10 MPa causes a reduction of intensity of between 10 and 50%, depending on window geometry. Specimens can be mounted on a removeable frame, and the solution in the sample chamber can be changed without disassembling the cell. The apparatus has been used successfully to study the low-angle diffraction from muscle.

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