Investigation of Fiber-Driven Mechanical Behavior of Human and Porcine Bladder Tissue Tested Under Identical Conditions

2021 ◽  
Author(s):  
Tyler G Tuttle ◽  
Duncan Morhardt ◽  
Andrea Poli ◽  
John M. Park ◽  
Ellen M. Arruda ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Smooth Muscle ◽  
Urinary Bladder ◽  
Mechanical Characteristics ◽  
Large Deformations ◽  
Amorphous Matrix ◽  
Longitudinal Direction ◽  
Human Bladder ◽  
Bladder Tissue ◽  
Material Models

Abstract The urinary bladder is a highly dynamic organ, that undergoes large deformations several times a day. Mechanical characteristics of the tissue are crucial in determining the function, and dysfunction, of the organ. Yet, literature reporting on the mechanical properties of human bladder tissue is scarce and, at times, contradictory. In this study, we focused on mechanically testing tissue from both human and pig bladders using identical protocols, to validate the use of pigs as a model for the human bladder. Furthermore, we tested the effect on tissue mechanical properties of two treatments, elastase to digest elastin fibers and oxybutynin to reduce smooth muscle cells spasticity, as well as of the anatomical direction of testing. We also implemented two different material models to aid in the interpretation of the experimental results. We found that human tissue behaves similarly to pig tissue at high deformations (collagen-dominated behavior) while we detected differences between the species at low deformations (amorphous matrix-dominated behavior). Our results also suggest that elastin could play a role in determining the behavior of the collagen fibers network. Finally, we confirmed the anisotropy of the tissue which reached higher stresses in the transverse when compared to the longitudinal direction.

Active Biaxial Mechanical Properties of Bladder Wall Tissue

2003 ◽  
Author(s):  
Jiro Nagatomi ◽  
Michael B. Chancellor ◽  
Michael S. Sacks
Keyword(s):  
Mechanical Properties ◽  
Smooth Muscle ◽  
Urinary Bladder ◽  
Muscle Tone ◽  
Bladder Wall ◽  
Upper Urinary Tract ◽  
Biaxial Stress ◽  
Bladder Tissue ◽  
Biaxial Mechanical Properties

The urinary bladder is a smooth muscle organ whose main functions are to store and to void urine. Since the most important aspect of the storage function of the bladder is to maintain low intravesical pressure in order to protect the upper urinary tract from backflow of urine, the compliance of the bladder wall is one of the key functional paramters to assess the health of this organ. Previously, our laboratory reported, for the first time, the biaxial mechanical properties of bladder wall tissue in the inactive state (in the absence of calcium in the testing bath solution and thus smooth muscle contraction was abolished) (Gloeckner et al. 2002). The bladder in vivo, however, normaly exhibits passive smooth muscle tone during filling and active contraction during voiding. Therefore, in order to completely characterize the bladder tissue mechanical behaviors, it is necessary to examine the load-deformation relationship of the bladder under the passive and active states. In the present study, a novel experimental model was designed to allow collection of biaxial stress-strain data from urinary bladder wall tissue under passive, active and inactive states.

scholarly journals Active mechanical properties of the smooth muscle of the urinary bladder

1979 ◽  
Vol 17 (3) ◽  
pp. 281-290 ◽  
Author(s):  
D. J. Griffiths ◽  
R. van Mastrigt ◽  
W. A. van Duyl ◽  
B. L. R. A. Coolsaet
Keyword(s):  
Mechanical Properties ◽  
Smooth Muscle ◽  
Urinary Bladder

In-vitro characterization of the pharmacological effects induced by (–)-α-bisabolol in rat smooth muscle preparations

2012 ◽  
Vol 90 (1) ◽  
pp. 23-35 ◽  
Author(s):  
Rodrigo J.B. de Siqueira ◽  
Walter B.S. Freire ◽  
Alfredo A. Vasconcelos-Silva ◽  
Patrícia A. Fonseca-Magalhães ◽  
Francisco J.B. Lima ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Biologically Active ◽  
Pharmacological Effects ◽  
Bladder Tissue ◽  
In Vitro Characterization ◽  
Voltage Dependent ◽  
Spontaneous Contractions

The present study deals with the pharmacological effects of the sesquiterpene alcohol (–)-α-bisabolol on various smooth-muscle preparations from rats. Under resting tonus, (–)-α-bisabolol (30–300 µmol/L) relaxed duodenal strips, whereas it showed biphasic effects in other preparations, contracting endothelium-intact aortic rings and urinary bladder strips, and relaxing these tissues at higher concentrations (600–1000 µmol/L). In preparations precontracted either electromechanically (by 60 mmol/L K+) or pharmacomechanically (by phenylephrine or carbachol), (–)-α-bisabolol showed only relaxing properties. The pharmacological potency of (–)-α-bisabolol was variable, being higher in mesenteric vessels, whereas it exerted relaxing activity with a lesser potency on tracheal or colonic tissues. In tissues possessing spontaneous activity, (–)-α-bisabolol completely decreased spontaneous contractions in duodenum, whereas it increased their amplitude in urinary bladder tissue. Administered in vivo, (–)-α-bisabolol attenuated the increased responses of carbachol in tracheal rings of ovalbumin-sensitized rats challenged with ovalbumin, but was without effect in the decreased responsiveness of urinary bladder strips in mice treated with ifosfamide. In summary, (–)-α-bisabolol is biologically active in smooth muscle. In some tissues, (–)-α-bisabolol preferentially relaxed contractions induced electromechanically, especially in tracheal smooth muscle. The findings from tracheal rings reveal that (–)-α-bisabolol may be an inhibitor of voltage-dependent Ca2+ channels.

Mechanical Characteristics of Electrospun Aligned PCL/PLLA Nanofibrous Scaffolds Conduct Cell Differentiation in Human Bladder Tissue Engineering

2013 ◽  
Vol 13 (7) ◽  
pp. 4736-4743 ◽  
Author(s):  
Hana Hanaee Ahvaz ◽  
Hamid Mobasheri ◽  
Behnaz Bakhshandeh ◽  
Nasser Shakhssalim ◽  
Mohammad Naji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Differentiation ◽  
Mechanical Characteristics ◽  
Human Bladder ◽  
Bladder Tissue ◽  
Nanofibrous Scaffolds

Temporal diabetes- and diuresis-induced remodeling of the urinary bladder in the rat

2006 ◽  
Vol 291 (3) ◽  
pp. R837-R843 ◽  
Author(s):  
Guiming Liu ◽  
Firouz Daneshgari
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Sprague Dawley ◽  
Muscle Area ◽  
Bladder Tissue ◽  
Cross Sectional ◽  
Wall Area ◽  
Total Cross Sectional Area ◽  
History Of ◽  
Physical Changes

The natural history of diabetes mellitus-induced remodeling of the urinary bladder is poorly understood. In this study, we examined temporal remodeling of the bladder in diabetic and diuretic rats. Male Sprague-Dawley rats were divided into three groups: streptozotocin-induced diabetic, 5% sucrose-induced diuretic, and age-matched control. Micturition and morphometric characteristics were evaluated using metabolic cages and light-microscopic examination of the bladder 4 days and 1, 2, 3, and 9 wk after induction. Digital image analysis was used to quantify equatorial cross-sectional areas of bladder tissue and lumen, as well as relative content of the three primary tissue components: smooth muscle, urothelium, and collagen. Diabetes and diuresis caused significant increases in fluid intake, urine output, and bladder weight. In both groups, progressive increases were observed in lumen area from 4 days to 3 wk after induction and in wall area from 2 to 3 wk after induction. Wall thickness decreased within the first 2 wk in the diabetic and diuretic rats but returned to control at 3 and 9 wk. As a percentage of total cross-sectional area, smooth muscle area increased, urothelium area was unchanged, and collagen area decreased in diabetic and diuretic rats after 2–3 wk compared with control rats. In conclusion, diabetes and diuresis induced similar bladder remodeling. Diabetes-induced diuresis caused adaptive physical changes in rat bladder by 4 days after induction; remodeling was observed by 2–3 wk after induction and remained stable from 3 to 9 wk.

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