Laser Surface Modification of Rare Earth Containing Magnesium Alloy Through Simulated Body Fluid and Its Impact on Cell Viability

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Khadka Indira ◽  
Wang Zhongke ◽  
Zheng Hongyu ◽  
Castagne Sylvie
Keyword(s):  
Rare Earth ◽  
Simulated Body Fluid ◽  
Cell Viability ◽  
Surface Treatment ◽  
Laser Irradiation ◽  
Body Fluid ◽  
Laser Surface ◽  
Test Method ◽  
Surface Structures ◽  
Direct Laser

Magnesium alloys have a good potential as structural biomaterials for temporary implant applications because of their self-degradation properties and biocompatibility. The surface condition is important for such applications, and lasers are often used to modify the surface characteristics of such components. In this context, the media through which the laser beam passes before reaching the surface to be irradiated is also of interest. In particular, laser irradiation in liquids affects the thermal energy delivery to the surface of the material, which in turns influences the formation of surface structures. In this work, rare earth containing WE54 Mg alloy has been irradiated under air and through a simulated body fluid (SBF) layer using a 500 watt pulsed Nd: YAG laser. As compared to direct laser surface treatment through air, laser irradiation through SBF generates new surface structures and deposition of ions issued from the SBF solution. Scanning electron microscope combined with energy dispersive spectroscopy (EDS) was used for the examination of surface structures formation and determination of elemental composition. Mesenchymal stem cells (MSC) culture was performed on laser modified WE54 alloy surface, and the MSC cytocompatibility on SBF-treated substrates was evaluated by the PrestoBlue™ assay test method. Cell reproducibility was observed on the SBF laser-treated surface which indicated that cell viability was improved by the surface treatment. The deposition of calcium and phosphorus ions on the WE54 surface was beneficial for cell viability. These results motivate the potential use of SBF-based films for biomedical purposes.

scholarly journals Laser irradiation of Mg-Al-Zn alloy: Reduced electrochemical kinetics and enhanced performance in simulated body fluid

2017 ◽  
Vol 12 (2) ◽  
pp. 021003 ◽  
Author(s):  
David C. Florian ◽  
Michael A. Melia ◽  
Fritz W. Steuer ◽  
Bruce F. Briglia ◽  
Michael K. Purzycki ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Laser Irradiation ◽  
Body Fluid ◽  
Electrochemical Kinetics ◽  
Zn Alloy

Evaluation of the Effects of Ag Ion Concentration on Osteoblast Activity

2014 ◽  
Vol 631 ◽  
pp. 390-394 ◽  
Author(s):  
S.B. Cho ◽  
G.J. Yoon ◽  
E.M. An ◽  
Y.J. Kim ◽  
T.N. Kim ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Cell Viability ◽  
Body Fluid ◽  
Neutral Red ◽  
Ion Concentration ◽  
Cell Counting ◽  
Osteoblast Activity ◽  
Cellular Behaviors ◽  
Covering Method

We reported the apatite-forming ability of 30CaO∙70SiO2 scaffolds with 0~100 ppm Ag ions by soaking in simulated body fluid (SBF). This study was to evaluate the effects of the concentrations of Ag ions in the 30CaO∙70SiO2 gels on in-vitro biocompatibility of osteoblasts (MC3T3). After seeding cells on the surface of Ag-30CaO∙70SiO2 gels scaffold, cellular behaviors were evaluated by an assay of cell counting kit-8. Cytotoxicity of the scaffold samples was evaluated by employing the extract solutions of the scaffold samples by the assays of neutral red, MTT and BrdU. In addition, live & dead assay was performed by using a gel covering method, which the scaffolds have been directly contacted with the incubated cells on the well plate. According to the results of CCK-8 assay, the optical density value of the absorbance of the resulting solution decreased as the concentration of Ag ions in the scaffolds increased. Moreover, their cell viability was measured to be less than 50% at the Ag concentrations of 50 ppm or more, and dead cells were observed in the experiment results of both the cytotoxicity and gel covering tests. From these experimental results, we concluded that the Ag-30CaO∙70SiO2 scaffolds with less than 50 ppm Ag ion concentration were considered as biocompatible.

Enhancing Corrosion Resistance of Stainless Steel 304 Using Laser Surface Treatment

2008 ◽  
Vol 384 ◽  
pp. 157-183 ◽  
Author(s):  
Ibrahim M. Ghayad ◽  
Madiha Shoeib ◽  
Taha Mattar ◽  
Huda M. Hussain
Keyword(s):  
Stainless Steel ◽  
Corrosion Resistance ◽  
Surface Treatment ◽  
Laser Irradiation ◽  
Laser Treatment ◽  
Surface Composition ◽  
Specific Treatment ◽  
Laser Surface ◽  
Laser Surface Treatment ◽  
Aisi 304

Stainless steel AISI 304 was laser treated to enhance corrosion resistance and improve surface properties. . This alloy has many applications in auto industry (car body,) as well as oil and gas industry. Different conditions were applied in the laser surface treatment, namely: laser power density, scan speed, distance between paths, medium gas (air, argon and nitrogen). After laser treatment, the samples microstructures were investigated using optical microscope to examine micro structural changes due to laser irradiation. Specimen surfaces were investigated using XRD, SEM and EDAX before and after laser treatment to examine the surface composition changes brought by laser irradiation. Results showed that laser irradiation enhances the corrosion resistance of AISI 304 Stainless steel to a large extent. Corrosion rates as low as 0.011 mpy for laser treated samples were obtained in comparison to 0.952 mpy obtained for the untreated samples. Superior pitting corrosion resistance was obtained under specific treatment conditions. The enhancement of corrosion resistance depends on the laser irradiation conditions. The corrosion protection afforded by laser treatment is attributed mainly to the grain refinement of the top surface layer. This layer is found to consist of nano-scale grains.

scholarly journals Nghiên cứu tính chất vật liệu thủy tinh y sinh 45S tổng hợp từ nguyên liệu chính cát trắng

2017 ◽  
Vol 33 (2) ◽  
Author(s):  
Bùi Xuân Vương
Keyword(s):  
Simulated Body Fluid ◽  
Cell Viability ◽  
Bioactive Glass ◽  
Body Fluid ◽  
In Vitro Bioactivity ◽  
Melting Method ◽  
Good Cell ◽  
Glass Powders

A bioactive glass 46S6 with composition 46% SiO2 - 24% CaO - 24% Na2O - 6% P2O5 (wt%) was elaborated by melting method. ‘‘In vitro’’ bioactivity of bio-glass was evaluated by soaking of glass-powders in a simulated body fluid (SBF) at different times. The obtained results highlighted the bioactivity of the bio-glass by the formation of a bioactive hydroxyapatite (HA) layer on its surface. Experiments ''in vitro'' in the presence of cells confirmed the non-toxicity and the good cell viability on this bio-glass.

Fatigue and Fretting Fatigue Behavior of Metallic Biomaterials

2010 ◽  
Vol 638-642 ◽  
pp. 618-623
Author(s):  
Norio Maruyama
Keyword(s):  
Tensile Strength ◽  
Fatigue Strength ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Fatigue Behavior ◽  
Fretting Fatigue ◽  
Test Method ◽  
Fatigue Properties ◽  
Metallic Biomaterials ◽  
The Relationship

A fretting fatigue test method in a simulated body fluid is shown to evaluate fatigue properties of metallic materials which are used in the orthopaedics field. Next, fatigue/fretting fatigue behavior in a simulated body fluid is given for 316L stainless steel, Ti-6% Al-4% V alloy, pure Ti for industrial use and Co-Cr-Mo alloy. Finally, we discuss the relationship between the tensile strength and the fatigue strength/fretting fatigue strength of metallic biomaterials at 107 cycles in air and in a simulated body fluid. For all of the biomaterials tested, the fatigue strength at 107 cycles is similar in air and in a simulated body fluid. The fatigue strength is closely correlated to the tensile strength: The fatigue strength increases with increasing tensile strength. However, a correlation is not observed between the fretting fatigue strength at 107 cycles and the fatigue strength or the tensile strength.

scholarly journals Laser irradiation of WE54 surface through simulated body fluid layer

2017 ◽  
Author(s):  
Indira Khadka ◽  
Sylvie Castagne ◽  
Zhongke Wang ◽  
Hongyu Zheng ◽  
Kuddannaya Shreyas
Keyword(s):  
Simulated Body Fluid ◽  
Laser Irradiation ◽  
Body Fluid ◽  
Fluid Layer
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