scholarly journals An Alternative Method to Characterize the Quasi-Static, Nonlinear Material Properties of Murine Articular Cartilage

2017 ◽  
Vol 140 (1) ◽  
Author(s):  
Alexander Kotelsky ◽  
Chandler W. Woo ◽  
Luis F. Delgadillo ◽  
Michael S. Richards ◽  
Mark R. Buckley
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Objective Measure ◽  
Nonlinear Material ◽  
Solid Matrix ◽  
Strain Mapping ◽  
Element Analysis ◽  
Static Mechanical ◽  
Tissue Degradation ◽  
Inverse Finite Element Analysis

With the onset and progression of osteoarthritis (OA), articular cartilage (AC) mechanical properties are altered. These alterations can serve as an objective measure of tissue degradation. Although the mouse is a common and useful animal model for studying OA, it is extremely challenging to measure the mechanical properties of murine AC due to its small size (thickness < 50 μm). In this study, we developed novel and direct approach to independently quantify two quasi-static mechanical properties of mouse AC: the load-dependent (nonlinear) solid matrix Young's modulus (E) and drained Poisson's ratio (ν). The technique involves confocal microscope-based multiaxial strain mapping of compressed, intact murine AC followed by inverse finite element analysis (iFEA) to determine E and ν. Importantly, this approach yields estimates of E and ν that are independent of the initial guesses used for iterative optimization. As a proof of concept, mechanical properties of AC on the medial femoral condyles of wild-type mice were obtained for both trypsin-treated and control specimens. After proteolytic tissue degradation induced through trypsin treatment, a dramatic decrease in E was observed (compared to controls) at each of the three tested loading conditions. A significant decrease in ν due to trypsin digestion was also detected. These data indicate that the method developed in this study may serve as a valuable tool for comparative studies evaluating factors involved in OA pathogenesis using experimentally induced mouse OA models.

Permeability of Human Glenohumeral Joint Cartilage

1999 ◽  
Author(s):  
Anna Stankiewicz ◽  
Gerard A. Ateshian ◽  
Louis U. Bigliani ◽  
Van C. Mow
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Interstitial Fluid ◽  
Glenohumeral Joint ◽  
Solid Matrix ◽  
Joint Cartilage ◽  
Frictional Drag ◽  
Diarthrodial Joints ◽  
Flow Through ◽  
Fluid Pressurization

Abstract The nearly frictionless lubrication in diarthrodial joints and load support within articular cartilage depends on its mechanical properties. It has been shown that the majority of applied loads on cartilage are supported by interstitial fluid pressurization (Ateshian et al., 1994) which results from the frictional drag of flow through the porous permeable solid matrix. The duration and magnitude of this pressurization are a function of the permeability of cartilage (Lai et al., 1981).

Use of Indentation Test to Determine the Proteoglycan Content of Articular Cartilage

2002 ◽  
Author(s):  
Xin Lu ◽  
Daniel D. Sun ◽  
X. Edward Guo ◽  
Hui Chen ◽  
W. Michael Lai ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Indentation Test ◽  
Solid Matrix ◽  
Interstitial Fluid Flow ◽  
Fibrous Network ◽  
Indentation Experiment ◽  
Proteoglycan Content ◽  
Creep And Stress Relaxation ◽  
First Time

The indentation experiment has been widely used to determine mechanical properties of articular cartilage [e.g., 1–3]. This method does not disrupt the fibrous network of the tissue nor does it require removing the tissue from the underlying bone. The biphasic indentation theory has been successfully used to determine the effect of interstitial fluid flow and pressurization (load support) on the creep and stress-relaxation behaviors of articular cartilage, and to determine its apparent mechanical properties (i.e., the elastic moduli of the extracellular solid matrix and its permeability) [1, 3]. However, due to its proteoglycan content, articular cartilage is a charged tissue with a high fixed charge density (FCD) [4]. Proteoglycan and collagen contents, water, etc, vary with age or with orthteoarthritis [4, 5]. The FCD plays important physicochemical roles in load support and mechano-electrochemial behaviors of the tissue and also regulates chondrocyte biosynthetic activities [4–7]. It is therefore important to develop an effective technique to determine not only the mechanical properties but also the electrochemical property (e.g., FCD) of the tissue, simultaneously and at the same location. The purpose of the current study is to determine, for the first time, both the mechanical properties and FCD of the extracellular matrix using an indentation test.

scholarly journals Effects of radiopharmaceuticals on articular cartilage’s mechanical properties

Nukleonika ◽  
2019 ◽  
Vol 64 (2) ◽  
pp. 71-74
Author(s):  
Nihal Kuzu ◽  
Ekrem Cicek
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Ionizing Radiation ◽  
Mechanical Tests ◽  
Cartilage Tissue ◽  
Proteoglycan Synthesis ◽  
Bovine Articular Cartilage ◽  
Technetium 99M ◽  
Static Mechanical ◽  
Radiation Science

Abstract As radiation science and technology advances, nuclear medicine applications are increasing worldwide which necessitate the understanding of biological implications of such practices. Ionizing radiation has been shown to cause degraded matrix and reduced proteoglycan synthesis in cartilage, and the late consequences of which may include degenerative arthritis or arthropathy. Although degenerative effects of the ionizing radiation on cartilage tissue have been demonstrated, the effects on the mechanical properties of articular cartilage are largely unknown. The radiopharmaceuticals, technetium-99m and technetium-99m sestamibi, were utilized on bovine articular cartilage to investigate these effects. We used two different mechanical tests to determine the mechanical properties of articular cartilage. Dynamic and static mechanical tests were applied to calculate compressive modulus for articular cartilage. We observed clearly higher control modulus values than that of experimental groups which account for lesser stiffness in the exposed cartilage. In conclusion, compressive moduli of bovine articular cartilage were found to decrease after radiopharmaceutical exposure, after both instantaneous and equilibrium mechanical experiments.

Hyperelastic compressive mechanical properties of the subcalcaneal soft tissue: An inverse finite element analysis

2016 ◽  
Vol 49 (7) ◽  
pp. 1186-1191 ◽  
Author(s):  
Vara Isvilanonda ◽  
Joseph M. Iaquinto ◽  
Shruti Pai ◽  
Peter Mackenzie-Helnwein ◽  
William R. Ledoux
Keyword(s):  
Mechanical Properties ◽  
Finite Element Analysis ◽  
Finite Element ◽  
Soft Tissue ◽  
Element Analysis ◽  
Inverse Finite Element Analysis
Sign in / Sign up

Export Citation Format

Share Document