scholarly journals Silver Nanoparticle Formation on Metal Substrate Under Concentration-Limited Condition

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Yong X. Gan ◽  
Gustavo R. Tavares ◽  
Rafhael S. Gonzaga ◽  
Ryan N. Gan
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Pure Aluminum ◽  
Glass Tube ◽  
Silver Ion ◽  
Ion Concentration ◽  
Limited Condition ◽  
Self Assembling ◽  
Working Electrode ◽  
Average Size

Silver nanoparticles were electrodeposited from 0.3 M oxalic acid electrolyte on a pure aluminum working electrode under silver ion concentration-limited condition. A silver wire was held in a glass tube containing 1.0 M KCl solution as the counter electrode. Ion exchange between the glass tube and the main electrodeposition bath through a capillary was driven by the overpotentials as high as 10 V supplied by an electrochemical workstation. Due to the reaction between chlorine anion and silver cation to form AgCl solid at the Ag/AgCl electrode, the silver ion concentration-limited condition holds in the electrolyte. It is found that silver grows at the aluminum working electrode to form nanoparticles with an average size of about 52.4 ± 13.6 nm. With the increasing of the deposition time, the silver nanoparticles aggregate into clusters. The silver particle clusters are separated with approximately 112.6 ± 19.7 nm due to the hydrogen bubble-induced self-assembling, which is shown by the confined deposition of silver on a gold coating. The surface roughness of the aluminum substrate leads to the reduced uniformity of silver nanoparticle nucleation and growth.

scholarly journals Biomimetic Synthesis of Silver Nanoparticles from Aqueous Extract of Saraca indica and its Profound Antibacterial Activity

2020 ◽  
Vol 11 (1) ◽  
pp. 8110-8120
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Silver Nanoparticle ◽  
Pathogenic Bacteria ◽  
Drug Formulation ◽  
Biomimetic Synthesis ◽  
Visible Absorption ◽  
The Stability ◽  
Average Size

The present findings were focused on green synthesis of silver nanoparticles through an aqueous extract of Saraca indica. The stability of the nanoparticle was achieved through the optimization of physico-chemical parameters. The sharp UV-visible absorption maximum at 400 was observed for biological synthesized silver nanoparticles. The spectroscopic analysis was thus used to assess the formation of silver nanoparticles. The AFM analysis did analyze the morphology of the nanocomposite, which was further confirmed through TEM micrograph. The electron micrograph image discloses that silver nanoparticles were polydispersed and dominantly as spherical with size ranges from 40nm to 100nm. The average size distribution was 49nm. The chemical reductions of Ag+ ions were further confirmed through FTIR. The biogenic silver nanoparticle and their drug formulation showed profound antibacterial activity against pathogenic bacteria. The flavonoids rich binding of silver nanoparticle showed great medicinal potential and can be used for the treatment of several harmful infectious diseases. Hence, plant-based metal nanoparticles meet the demand for less toxic formulation during drug development and its delivery.

Impacts of broth chemistry on silver ion release, surface chemistry composition, and bacterial cytotoxicity of silver nanoparticles

2018 ◽  
Vol 5 (2) ◽  
pp. 304-312 ◽  
Author(s):  
Kathryn A. Johnston ◽  
Lisa M. Stabryla ◽  
Ashley M. Smith ◽  
Xing Yee Gan ◽  
Leanne M. Gilbertson ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Surface Chemistry ◽  
Bacterial Growth ◽  
Silver Nanoparticle ◽  
Silver Ion ◽  
Growth Media ◽  
Ion Release ◽  
Nanoparticle Surface ◽  
The Impact ◽  
Silver Ion Release

We determine the impact of bacterial growth media on silver nanoparticle surface chemistry, this surface chemistry on silver ion release from the nanoparticles, and ultimately the antimicrobial implications of those parameters.

Synthesis of Silver Nanoparticles at the Liquid–Liquid Using Ultrasonic Wave

2013 ◽  
Vol 135 (1) ◽  
Author(s):  
Seong-Ik Hong ◽  
Alma Duarte ◽  
Gabriel A. Gonzalez ◽  
Nam-Soo Kim
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
New Technologies ◽  
Ultrasonic Waves ◽  
Reducing Agent ◽  
Liquid Interface ◽  
Silver Ion ◽  
Nanoparticle Size ◽  
Ion Movement

The high demand of flexible electronics and the miniaturization of electronic components have been increasing very rapidly. Nanotechnology and in particular nanoparticles have become very important for the development of new technologies and applications which depend on the synthesis and characterization of nanoparticles with specific properties. Significant attention has been focused on the characteristics of the nanoparticles since their properties, particle size and shape are very different when compared to those of the bulk materials. In order to produce nanoparticles with more efficient structures and electronic properties for nano ink, it is necessary to control the particle size to avoid agglomeration. Currently, the nanoparticle size and its agglomeration is controlled by surfactants, but some studies have shown that adding surfactants have negative effects on the conductivity of the nanoparticles along with the high curing temperatures of nano-ink. In this study, silver nanoparticles were synthesized by adding methanol to water instead of surfactants in order to control the silver nanoparticle size. The water and methanol solution was prepared by using different ratios v/v of water/methanol obtaining a liquid–liquid interface and forming a molecular–molecular interface restricting the silver ion movement in the solution. In addition to the liquid–liquid interface, the ion concentration and movement were also restricted through a spraying mist by using ultrasonic waves. The silver ion and the reducing agent were found to have a difference in concentration by the spraying mist method. The movement of the silver ions and the reducing agents were controlled by the difference in concentration. It was observed that the control of the nanoparticles and ion movement was more efficient by spraying silver ion solution than using a reducing agent solution. We confirmed that by increasing the volumetric ratios of methanol the silver nanoparticle size also increased. The mist particle size and the concentration were also calculated at different volumetric ratios of methanol.

scholarly journals The role of an encapsulin nanocompartment in resisting silver ion stress

2020 ◽  
Author(s):  
Chong-Yang Xing ◽  
Teng-Fei Ma ◽  
Jinsong Guo ◽  
Yu Shen ◽  
Peng Yan ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Silver Ions ◽  
Silver Ion ◽  
Ion Concentration ◽  
Toxic Substances ◽  
Label Free ◽  
Self Assembling ◽  
In The Wild ◽  
Heavy Metal Pollutants

Abstract Background: Compartmentalization can protect cells from the interference of external toxic substances by sequestering toxic products. We hypothesized that proteinaceous nanocompartments may be a feasible candidate material to be added to genetically modified bacteria for the sequestration of toxic environmental products, which would open up a new biological detoxification pathway. Results: Here, we identified a new mechanism by which bacteria resist silver ion stress. We showed that the self-assembling nanocompartments helped a model species ( E. coli ) resist silver ion stress. Transmission electron microscopy and energy dispersive X-ray (TEM-EDX) analysis showed that the nanocompartments combined stably with silver ions in vitro . In addition, when exposed to 30 μM AgNO 3 , the survival rate of genetically modified bacteria (with nanocompartments) was 86%, while it was just 59% in the wild-type bacteria (without nanocompartments). Label-free quantitative proteomics indicated that the nanocompartments enhanced bacterial activity by inducing the up-regulation of protein processing and secondary metabolites, and decreased their intracellular silver ion concentration, both of which contributed to their increased resistance to toxic silver ions. Conclusions: This study on nanocompartments has contributed to a deeper understanding of how bacteria respond to environmental stressors like heavy metal pollutants in water, soil, and sediment. The encapsulin nanocompartment has the potential to be applied in various environments.

scholarly journals ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES AGAINST Aeromonas spp. AND Vibrio spp. ISOLATED FROM AQUACULTURE WATER ENVIRONMENT IN THUA THIEN HUE

2019 ◽  
Vol 128 (3B) ◽  
Author(s):  
Nguyen Van Khanh ◽  
Phan Van Cu
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Nanoparticle ◽  
Vibrio Harveyi ◽  
Water Environment ◽  
White Shrimp ◽  
Fresh Water Fish ◽  
Fish Ponds ◽  
Average Size ◽  
Vibrio Spp

Abstract: A silver nanoparticle solution prepared at the Center for Incubation and Technology Transfers was used in the current study. The nanoparticles have an average size of 15.0 nm. The silver nanoparticle solution exhibits an antibacterial activity to Aeromonas hydrophyla and Aeromonas caviae isolated from fresh water fish ponds and Vibrio harveyi and Vibrio alginoliticus isolated from white shrimp ponds. The silver nanoparticle solution at a concentration of 25 ppm inhibits A. caviae and A. hydrophila, and the peak attenuation time was 24 hours after exposure to the bacteria. The solution at a concentration of 12.5 ppm also inhibits Vibrio harveyi and Vibrio alginoliticus, and the peak attenuation time was 48 hours after exposure to the bacteria.Keywords: antibacterial activity, silver nanoparticles, Aeromonas spp. and Vibrio spp.

scholarly journals Green Synthesis of Silver Nanoparticles by Sonicated Method Using Pulicha Indeca Stem Extract: Characterization and Study of Antibacterial Activity, Anticancer Activity and Cell Viability Test

2019 ◽  
Vol 35 (6) ◽  
pp. 1767-1773 ◽  
Author(s):  
Gugulothu Yaku ◽  
Venkateswerlu Venkateswerlu ◽  
TVD Prasad Rao
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Anticancer Activity ◽  
Nano Particles ◽  
Ion Concentration ◽  
Tem Analysis ◽  
Stem Extract ◽  
Average Size ◽  
Green Synthesized Silver Nanoparticles ◽  
Size Distribution Histogram

In the present work, green synthesized silver nanoparticles by sonofication method using pulicha extract, which can act as reducing as well as stabilizing agent. The silver nanoparticles were characterized by UV-VIS spectra, FTIR, XRD, SEM and TEM analysis. The UV –VIS SPR peak was observed at 440 nm, which represents the characteristic plasmon resonance of nanostructures. The fcc crystalline structure of silver nanoparticles is evident from XRD studies. The shape and size of synthesized AgNPs were studied by TEM. The synthesized AgNPs, were mostly mono-dispersed and spherical in shape. The particles are well separated from each other and did not exhibit any aggregation. This indicates the effective capping nature of pulchea indeca extract. The AgNPs size distribution histogram and the average size of AgNPs was found to be 14 ± 2 nm. The effect of silver ion concentration range from 1mm to 10mm and extract concentration 1% to 8% has been studied on the formation of silver nano particles. The AgNPs shows positive antibacterial acivity against stayphylococusaureoous , klebisellapneumonia, basilusbtilis and negative antibacterial activity against protious mirabilis bacteria studied. The anticancer activity of AgNps shows positive activity on HeLa cell.

Detection of intracellular silver nanoparticles using flow cytometry

2018 ◽  
pp. 223-226
Author(s):  
С.И. Каба ◽  
А.А. Соколовская
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Endothelial Cells ◽  
Silver Nanoparticle ◽  
Cell Uptake ◽  
Control Samples

Продемонстрировано обнаружение наночастиц серебра во внутриклеточном пространстве с помощью проточной цитофлуориметрии. В эндотелиальных клетках линии EA.hy926, инкубированных в растворе, содержащем 2 мкг/мл наносеребра, измеряли боковое светорассеяние. По сравнению с контрольными образцами этот параметр возрастал, в то время как прочие значимые характеристики не изменялись. Это подтверждает чувствительность метода к изменившемуся состоянию клеток и указывает на поглощение наночастиц серебра клетками при концентрации ниже токсической. The study demonstrated a possibility for detection of intracellular silver nanoparticles using flow cytometry. The parameter used in this work, side scattering, was measured in EA.hy926 endothelial cells incubated in a 2 mg/ml silver nanoparticle solution. This parameter was increased compared to control samples. Therefore, this technique was sensitive to changes in the cell status and suggested the cell uptake of the particles under the subtoxic conditions.

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

scholarly journals A comparative study of silver nanoparticle dissolution under physiological conditions

2020 ◽  
Vol 2 (12) ◽  
pp. 5760-5768
Author(s):  
Lukas Steinmetz ◽  
Christoph Geers ◽  
Sandor Balog ◽  
Mathias Bonmarin ◽  
Laura Rodriguez-Lorenzo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Comparative Study ◽  
Silver Nanoparticle ◽  
Biologically Relevant ◽  
Physiological Conditions ◽  
Aggregation Behaviour

The dissolution and aggregation behaviour of silver nanoparticles under physiological conditions and in biologically relevant environments is investigated by exploiting their plasmonic properties.

scholarly journals Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3790
Author(s):  
Pratama Jujur Wibawa ◽  
Muhammad Nur ◽  
Mukhammad Asy’ari ◽  
Wijanarka Wijanarka ◽  
Heru Susanto ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Activated Carbon ◽  
Aloe Vera ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
E Coli ◽  
Transmission Electron ◽  
Average Size ◽  
Green Synthesized Silver Nanoparticles

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

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