scholarly journals Hybrid Tissue Engineering Scaffolds by Combination of Three-Dimensional Printing and Cell Photoencapsulation

2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Marica Markovic ◽  
Jasper Van Hoorick ◽  
Katja Hölzl ◽  
Maximilian Tromayer ◽  
Peter Gruber ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fused Deposition Modeling ◽  
Structural Parameters ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Tissue Engineering Scaffolds ◽  
Ecm Remodeling ◽  
Initial Cell ◽  
Fused Deposition ◽  
Hydrogel Matrix

Three-dimensional (3D) printing offers versatile possibilities for adapting the structural parameters of tissue engineering scaffolds. However, it is also essential to develop procedures allowing efficient cell seeding independent of scaffold geometry and pore size. The aim of this study was to establish a method for seeding the scaffolds using photopolymerizable cell-laden hydrogels. The latter facilitates convenient preparation, and handling of cell suspension, while distributing the hydrogel precursor throughout the pores, before it is cross-linked with light. In addition, encapsulation of living cells within hydrogels can produce constructs with high initial cell loading and intimate cell-matrix contact, similar to that of the natural extra-cellular matrix (ECM). Three dimensional scaffolds were produced from poly(lactic) acid (PLA) by means of fused deposition modeling. A solution of methacrylamide-modified gelatin (Gel-MOD) in cell culture medium containing photoinitiator Li-TPO-L was used as a hydrogel precursor. Being an enzymatically degradable derivative of natural collagen, gelatin-based matrices are biomimetic and potentially support the process of cell-induced remodeling. Preosteoblast cells MC3T3-E1 at a density of 10 × 106 cells per 1 mL were used for testing the seeding procedure and cell proliferation studies. Obtained results indicate that produced constructs support cell survival and proliferation over extended duration of our experiment. The established two-step approach for scaffold seeding with the cells is simple, rapid, and is shown to be highly reproducible. Furthermore, it enables precise control of the initial cell density, while yielding their uniform distribution throughout the scaffold. Such hybrid tissue engineering constructs merge the advantages of rigid 3D printed constructs with the soft hydrogel matrix, potentially mimicking the process of ECM remodeling.

Development of three-dimensional printing filaments based on poly(lactic acid)/hydroxyapatite composites with potential for tissue engineering

2021 ◽  
pp. 002199832098856
Author(s):  
Marcela Piassi Bernardo ◽  
Bruna Cristina Rodrigues da Silva ◽  
Luiz Henrique Capparelli Mattoso
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Fused Deposition Modeling ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Scanning Calorimetry ◽  
Three Dimensional Printing ◽  
Fused Deposition ◽  
Deposition Modeling ◽  
3D Printed

Injured bone tissues can be healed with scaffolds, which could be manufactured using the fused deposition modeling (FDM) strategy. Poly(lactic acid) (PLA) is one of the most biocompatible polymers suitable for FDM, while hydroxyapatite (HA) could improve the bioactivity of scaffold due to its chemical composition. Therefore, the combination of PLA/HA can create composite filaments adequate for FDM and with high osteoconductive and osteointegration potentials. In this work, we proposed a different approache to improve the potential bioactivity of 3D printed scaffolds for bone tissue engineering by increasing the HA loading (20-30%) in the PLA composite filaments. Two routes were investigated regarding the use of solvents in the filament production. To assess the suitability of the FDM-3D printing process, and the influence of the HA content on the polymer matrix, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) were performed. The HA phase content of the composite filaments agreed with the initial composite proportions. The wettability of the 3D printed scaffolds was also increased. It was shown a greener route for obtaining composite filaments that generate scaffolds with properties similar to those obtained by the solvent casting, with high HA content and great potential to be used as a bone graft.

Development of Scaffold Building Units and Assembly for Tissue Engineering Using Fused Deposition Modelling

2009 ◽  
Vol 83-86 ◽  
pp. 269-274 ◽  
Author(s):  
Syed H. Masood ◽  
Kadhim Alamara
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Fused Deposition Modeling ◽  
Porous Scaffold ◽  
Cell Structure ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Fused Deposition Modelling ◽  
Fused Deposition ◽  
Unit Cells

In tissue engineering (TE), a porous scaffold structure of biodegradable material is required as a template to guide the proliferation, growth and development of cells appropriately in three dimensions. The scaffold must meet design requirements of appropriate porosity, pore size and interconnected structure to allow cell proliferation and adhesion. This paper presents a methodology for design and manufacture of TE scaffolds with varying porosity by employing open structure building units and Fused Deposition Modeling (FDM) rapid prototyping technique. A computer modeling approach for constructing and assembly of three-dimensional unit cell structure is presented to provide a solution of scaffolds design that can potentially meet the diverse requirements of TE applications. A parametric set of open polyhedral unit cells is used to assist the user in designing the required micro-architecture of the scaffold with required porosity and pore size and then the Boolean operation is used to create the scaffold of a given CAD model from the designed microstructure. The procedure is verified by fabrication of physical scaffolds using the commercial FDM system.

scholarly journals Characterization of Three-Dimensional Printed Composite Scaffolds Prepared with Different Fabrication Methods

2016 ◽  
Vol 61 (2) ◽  
pp. 645-650 ◽  
Author(s):  
K. Szlązak ◽  
J. Jaroszewicz ◽  
B. Ostrowska ◽  
T. Jaroszewicz ◽  
M. Nabiałek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fused Deposition Modeling ◽  
Testing Machine ◽  
Three Dimensional ◽  
Gravimetric Analysis ◽  
Solution Casting ◽  
Micro Computed Tomography ◽  
Optimal Method ◽  
Fused Deposition ◽  
Material Testing Machine

Abstract An optimal method for composites preparation as an input to rapid prototyping fabrication of scaffolds with potential application in osteochondral tissue engineering is still needed. Scaffolds in tissue engineering applications play a role of constructs providing appropriate mechanical support with defined porosity to assist regeneration of tissue. The aim of the presented study was to analyze the influence of composite fabrication methods on scaffolds mechanical properties. The evaluation was performed on polycaprolactone (PCL) with 5 wt% beta-tricalcium phosphate (TCP) scaffolds fabricated using fused deposition modeling (FDM). Three different methods of PCL-TCP composite preparation: solution casting, particles milling, extrusion and injection were used to provide material for scaffold fabrication. The obtained scaffolds were investigated by means of scanning electron microscope, x-ray micro computed tomography, thermal gravimetric analysis and static material testing machine. All of the scaffolds had the same geometry (cylinder, 4×6 mm) and fiber orientation (0/60/120°). There were some differences in the TCP distribution and formation of the ceramic agglomerates in the scaffolds. They depended on fabrication method. The use of composites prepared by solution casting method resulted in scaffolds with the best combination of compressive strength (5.7±0.2 MPa) and porosity (48.5±2.7 %), both within the range of trabecular bone.

scholarly journals Research on the Application of MWCNTs/PLA Composite Material in the Manufacturing of Conductive Composite Products in 3D Printing

Micromachines ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 635 ◽  
Author(s):  
Jinjie Luo ◽  
Haibao Wang ◽  
Duquan Zuo ◽  
Anping Ji ◽  
Yaowen Liu
Keyword(s):  
Composite Material ◽  
3D Printing ◽  
Fused Deposition Modeling ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Melt Flow Rate ◽  
Conductive Composites ◽  
Fused Deposition ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

As an advanced manufacturing technology that has been developed in recent years, three-dimensional (3D) printing of macromolecular materials can create complex-shaped components that cannot be realized by traditional processing. However, only a few types of macromolecular materials are suitable for 3D printing: the structure must have a single function, and manufacturing macromolecular functional devices is difficult. In this study, using poly lactic acid (PLA) as a matrix, conductive composites were prepared by adding various contents of multi-walled carbon nanotubes (MWCNTs). The printability and properties of MWCNT/PLA composites with different MWCNT proportions were studied by using the fused deposition modeling (FDM) processing technology of 3D printing. The experimental results showed that high conductivity can be realized in 3D-printed products with a composite material containing 5% MWCNTs; its conductivity was 0.4 ± 0.2 S/cm, its tensile strength was 78.4 ± 12.4 MPa, and its elongation at break was 94.4% ± 14.3%. It had a good melt flow rate and thermal properties, and it enabled smooth printing, thus meeting all the requirements for the 3D printing of consumables.

A Robust True Direct-Print Technology for Tissue Engineering

2007 ◽  
Author(s):  
B. Li ◽  
T. Dutta Roy ◽  
C. M. Smith ◽  
P. A. Clark ◽  
K. H. Church
Keyword(s):  
Tissue Engineering ◽  
Computer Aided Design ◽  
Fused Deposition Modeling ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Fused Deposition ◽  
Wide Range ◽  
Computer Aided ◽  
Liquid Material ◽  
Print Process

Numerous solid freeform fabrication (SFF) or rapid prototyping (RP) techniques have been employed in the field of tissue engineering to fabricate specially organized three-dimensional (3-D) structures such as scaffolds. Some such technologies include, but are not limited to, laminated object manufacturing (LOM), three-dimensional printing (3-DP) or ink-jet printing, selective laser sintering (SLS), and fused deposition modeling (FDM). These techniques are capable of rapidly producing highly complex 3-D scaffolds or other biomedical structures with the aid of a computer-aided design (CAD) system. However, they suffer from lack of consistency and repeatability, since most of these processes are not fully controlled and cannot reproduce the previous work with accuracy. Also, these techniques (excluding FDM) are not truly direct-print processes. Certain material removing steps are involved, which in turn increases the complexity and the cost of fabrication. The FDM process has good repeatability; however, the materials that can be used are limited due to the high temperature needed to melt the feedstock. Some researchers also reported that the scaffolds fabricated by FDM lack consistency in the z-direction. In this paper, we will present a true direct-print technology for repeatedly producing scaffolds and other biomedical structures for tissue engineering with the aid of our Computer Aided Biological (CAB) tool. Unlike other SFF techniques mentioned above, our direct-print process fabricates scaffolds or other complex 3-D structures by extruding (dispensing) a liquid material onto the substrate with a prescribed pattern generated by a CAD program. This can be a layer-by-layer 2.5 dimension build or a true 3-D build. The dispensed liquid material then polymerizes or solidifies, to form a solid structure. The flexibility in the types of materials that can be extruded ranges from polymers to living cells, encapsulated in the proper material. True 3-D structures are now possible on a wide range of substrates, including even in vivo. Some of the advantages of the process are a) researchers have full control over the patterns to be created; b) it is a true direct-print process with no material removing steps involved; c) it is highly consistent and repeatable; and d) it is highly efficient and cost-effective. This paper will first give a detailed description of the CAB tool. Then, it will present a detailed process for printing polycaprolactone (PCL) into a defined 3-D architecture, where the primary focus for these constructs is for use in tissue engineering applications. Finally, mechanical characterization results of the printed scaffolds will be included in the paper.

NIST Development of Reference Material Scaffolds for Tissue Engineering

2005 ◽  
Author(s):  
Joy P. Dunkers ◽  
Stefan D. Leigh ◽  
Marcus T. Cicerone ◽  
Forrest A. Landis ◽  
Francis W. Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Reference Material ◽  
Fused Deposition Modeling ◽  
Three Dimensional ◽  
Test Methods ◽  
Medical Products ◽  
Fused Deposition ◽  
Polymeric Scaffolds ◽  
Scaffold Materials ◽  
Radiological Health

In consultation with ASTM and other stakeholders in Tissue-Engineered Medical Products (TEMPs) industry, the National Institute of Standards and Technology (NIST) initiated a project designed to produce Reference Material scaffolds for tissue engineering. The rationale for Reference Material scaffolds was developed through several NIST/Industry workshops. In brief, Reference Material scaffolds have multiple uses: facilitating the development and the validation of new test methods that measure interactions among various components of a TEMP; comparison with other scaffolds and scaffold materials in terms of cellular responses, biodegradation, and releases of growth factors; and comparisons of responses among various cell lines. The primary customers for Reference Material scaffolds are expected to be the TEMPs industry, academic researchers, regulators, and standards developing organizations. There are many properties of a TEMP that warrant development of multiple Reference Material scaffolds. Currently, NIST is defining a set of Reference Material scaffolds based on geometric descriptors such as permeability, pore volume, pore size distribution, interconnectivity, and tortuosity. In consultation with ASTM, NIST is testing three candidate scaffolds produced by: three dimensional (3-D) printing, stereolithography, and fused deposition modeling (FDM). Scaffolds made by these methods have been obtained from Mayo Clinic (Rochester, MN), Case Western Reserve University (CWRU) (Cleveland, OH), and Osteopore International (Singapore), respectively, for structural characterization. These prototype scaffolds, with well-defined architectures, have been selected to address the following items of interest: 1) establishment of useful functional definitions of porosity content, interconnectivity, and pores; 2) evaluation of testing methods listed in the Standard Guide for the Porosity of Polymeric Scaffolds for Use in Tissue-Engineered Medical Products, which is being drafted by ASTM. Currently, NIST and the Center for Devices and Radiological Health of the Food and Drug Administration, as well as other groups from US and foreign laboratories, are actively carrying out cross-validation test of these prototype scaffolds.

scholarly journals Impact of Aging Effect and Heat Treatment on the Tensile Properties of PLA (Poly Lactic Acid) Printed Parts

2020 ◽  
Vol 57 (3) ◽  
pp. 147-159
Author(s):  
Mohammad Sakib Hasan ◽  
Toni Ivanov ◽  
Milos Vorkapic ◽  
Aleksandar Simonovic ◽  
David Daou ◽  
...  
Keyword(s):  
Heat Treatment ◽  
3D Printing ◽  
Tensile Properties ◽  
Fused Deposition Modeling ◽  
Three Dimensional ◽  
Aging Effect ◽  
Poly Lactic Acid ◽  
Layer Height ◽  
Fused Deposition ◽  
Deposition Modeling

Rapid innovations in 3D printing technology have allowed highly complex parts to be manufactured quickly and easily, particularly for prototyping purposes. Fused Deposition Modeling of thermoplastic materials is one of the most commonly used techniques in three-dimensional (3D) printing. The major aim of Fused Deposition Modeling (FDM) is to design and manufacture usable parts for fields such as engineering and medicine. Therefore, it is essential to investigate the mechanical properties of such FDM processed structures. One of the most commonly used materials currently on the market is Polylactic Acid (PLA). The main purpose of this paper is to investigate the effects of aging and heat treatment on the tensile properties of PLA printed test specimens. The tensile properties of parts manufactured by the 3D printer are influenced by various parameters such as extrusion temperature, infill density, building direction, layer height, etc. A total of 96 specimens were built by altering building orientation and layer height to estimate and compare the tensile properties of the printed parts. To investigate the aging effect, 30 of 96 specimens were printed 6 months before the tensile experiment. Half of both aged and new specimens were cured in an oven at 57.5 [�ŞC] for 3 hours while the other half endured no heat treatment. After the performed measurement, it can be concluded that heat treatment generally improves structural strength of the printed parts, while aging decreases it. However, these effects are highly dependent on the layer thickness and printing quality. The tensile test is conducted according to the ASTM D638 standard. The fractured samples were further characterized using an electron microscope.

scholarly journals CUSTOMIZATION OF ELECTROSPINNING FOR TISSUE ENGINEERING

2018 ◽  
Vol 16 (3) ◽  
pp. 321
Author(s):  
Nenad Grujović ◽  
Fatima Živić ◽  
Matthias Schnabelrauch ◽  
Torsten Walter ◽  
Ralf Wyrwa ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fused Deposition Modeling ◽  
3D Printer ◽  
Tissue Engineering Scaffolds ◽  
Fused Deposition ◽  
Melt Electrospinning ◽  
Controlled Porosity ◽  
Deposition Modeling ◽  
Solution Electrospinning ◽  
Different Characteristics

This paper deals with two electrospinning technologies: the melt electrospinning with a customized jet head, adapted from the fused deposition modeling (FDM) 3D printer, in comparison with the standard solution electrospinning, aiming at fabrication of tissue engineering scaffolds. The resulting fibers are compared. The influence of the collector properties on those of the fabricated scaffold is investigated. The resulting electrospun fibers exhibit different characteristics such as morphology and thickness, depending on the technology. The micro-fibers are produced by the melt electrospinning with an inbuilt 3D printer jet head, whereas the solution electrospinning has produced nano- and micro-fibers. The scaffolds fabricated on the rotating drum collector exhibit a more ordered structure as well as thinner fibers than those produced on the stationary plate collector. Further investigations should aim at fabrication of porous hollow fibers and tissue engineering scaffolds with controlled porosity and properties.

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