Investigation of Hydrodynamic Focusing in a Microfluidic Coulter Counter Device

2012 ◽  
Vol 134 (8) ◽  
Author(s):  
Muheng Zhang ◽  
Yongsheng Lian ◽  
Cindy Harnett ◽  
Ellen Brehob
Keyword(s):  
Flow Field ◽  
Flow Rate ◽  
Coulter Counter ◽  
Hydrodynamic Focusing ◽  
Relative Flow Rate ◽  
Advection Diffusion Equation ◽  
Sample Stream ◽  
Voltage Signal ◽  
The Impact ◽  
Relative Flow

The Coulter technique enables rapid analysis of particles or cells suspended in a fluid stream. In this technique, the cells are suspended in an electrically conductive solution, which is hydrodynamically focused by nonconducting sheath flows. The cells produce a characteristic voltage signal when they interrupt an electrical path. The population and size of the cells can be obtained through analyzing the voltage signal. In a microfluidic Coulter counter device, the hydrodynamic focusing technique is used to position the conducting sample stream and the cells and also to separate close cells to generate distinct signals for each cell and avoid signal jam. The performance of hydrodynamic focusing depends on the relative flow ratio between the sample stream and sheath stream. We use a numerical approach to study the hydrodynamic focusing in a microfluidic Coulter counter device. In this approach, the flow field is described by solving the incompressible Navier-Stokes equations. The sample stream concentration is modeled by an advection-diffusion equation. The motion of the cells is governed by the Newton-Euler equations of motion. Particle motion through the flow field is handled using an overlapping grid technique. A numerical model for studying a microfluidic Coulter counter has been validated. Using the model, the impact of relative flow rate on the performance of hydrodynamic focusing was studied. Our numerical results show that the position of the sample stream can be controlled by adjusting the relative flow rate. Our simulations also show that particles can be focused into the stream and initially close particles can be separated by the hydrodynamic focusing. From our study, we conclude that hydrodynamic focusing provides an effective way to control the position of the sample stream and cells and it also can be used to separate cells to avoid signal jam.

scholarly journals Increase fish protective efficiency constructions of meliorative water intake

2020 ◽  
pp. 69-73
Author(s):  
Svetlana Mikhailovna Dragunova ◽  
Yevgeniy Vladimirovich, Кuznetsov ◽  
Anna Yevgenievna Khadzhidi
Keyword(s):  
Physical Model ◽  
Water Intake ◽  
Flow Rate ◽  
Juvenile Fish ◽  
Protective Efficiency ◽  
Irrigation Systems ◽  
Relative Flow Rate ◽  
Reclamation System ◽  
Relative Flow ◽  
Fish Protection

The article solves the problem of increasing the level of protection of juvenile fish to a standard indicator by the modernization of individual elements of fish protection of ameliorative water intake of irrigation systems. The design of an integrated fish-protecting structure with a logging boom adapted to hydrology and the rhythm of migration of juvenile fish from irrigation sources has been proposed. The results of studies on a physical model, taking into account the costs of the reclamation system, show an increase in the efficiency of the combined fish protection structure with a harbor for reclamation water intakes in the range of 78,5–84,0 %, depending on the relative flow rate on the shelf of the sanctuary.

The effect of repeated cold exposure of the hand on the reactivity of digital vessels

1969 ◽  
Vol 47 (3) ◽  
pp. 261-265 ◽  
Author(s):  
Peter Gaskell ◽  
Kathleen L. Long
Keyword(s):  
Blood Flow ◽  
Flow Rate ◽  
Cold Exposure ◽  
Opening Pressure ◽  
Relative Flow Rate ◽  
Opposite Hand ◽  
Critical Opening ◽  
Before And After ◽  
Relative Change ◽  
Relative Flow

One hand of each of 10 subjects was immersed in stirred water at 4 °C for 1 h per day, 5 days per week, for 3 weeks, to produce local acclimatization to cold. The opposite hand was immersed at the same time in water at 32 °C. The reactivity of digital vessels in a finger of each hand was measured as the increase in the critical opening pressure of the vessels in response to an intravenous infusion of noradrenaline at 2 and 5 μg/min. The reactivity of digital vessels in the test hand was compared with that in the control hand both before and after the repeated cold exposure of the test hand to see whether the cold exposure altered the reactivity of vessels in the test hand relative to that in the control hand. No evidence of such a relative change was obtained. Resting blood flow at 21 and 32 °C in the test hand was compared with that in the control hand both before and after the repeated cold exposure. No change in the relative flow rate in the two hands was observed as a result of the cold exposure. Roentgen studies of the hands did not reveal any effect of the repeated cold exposure on the mineralization of the bones of the hands.

scholarly journals Investigation of Low-Temperature Lean Combustion Characteristics in Power Plants with External Heating of Components

2021 ◽  
Vol 2(50) ◽  
Author(s):  
Nikolay Bachev ◽  
◽  
Alena Shilova ◽  
Oleg Matyunin ◽  
Oksana Betinskaya ◽  
...  
Keyword(s):  
Low Temperature ◽  
Combustion Chamber ◽  
Flow Rate ◽  
Turbulent Combustion ◽  
Power Plants ◽  
Lean Combustion ◽  
Relative Flow Rate ◽  
External Heating ◽  
Low Emission ◽  
Relative Flow

An integral part of any open-type gas turbine plant is a low-emission combustion chamber, which is usually two-zone and cooled. One of the ways to reduce emission of harmful substanc-es is organizing low-emission low-temperature lean combustion with external heating of compo-nents. This paper investigates the effect of external heating of air and fuel gas on expansion of the lower combustion limit and stable flame position in a single-zone uncooled combustion chamber of a microgas turbine power plant. Stable position of the flame front in combustion chambers of this type mainly depends on the ratio between the average flow rate of the combus-tible-air mixture and the rate of turbulent combustion. This ratio depends on thermal, gas-dynamic, thermochemical and geometric factors. The purpose of this work is to substantiate the possibility of using the relative flow rate as a generalized characteristic. This goal was achieved in processing a large amount of published experimental data and numerical modeling of low-temperature combustion of lean mixtures. The most significant research result is determination of the range of relative flow rate (gk = 0.3…3.5·10-4 kg⁄s∙N), at which it is possible to ensure sta-ble flame position in a single-zone combustion chamber. Significance of the obtained results lies in the fact that using the relative flow rate makes it possible to quickly determine and analyze the geometric and gas-dynamic parameters and characteristics of turbulent combustion in com-bustion chambers of micro-gas turbine power plants.

Millisecond timescale reactions observed via X-ray spectroscopy in a 3D microfabricated fused silica mixer

2021 ◽  
Vol 28 (4) ◽  
Author(s):  
Diego A. Huyke ◽  
Ashwin Ramachandran ◽  
Oscar Ramirez-Neri ◽  
Jose A. Guerrero-Cruz ◽  
Leland B. Gee ◽  
...  
Keyword(s):  
Residence Time ◽  
Flow Rate ◽  
Fused Silica ◽  
Diffusion Reaction ◽  
Low Flow ◽  
Epifluorescence Microscopy ◽  
Hydrodynamic Focusing ◽  
X Ray ◽  
Sample Stream

Determination of electronic structures during chemical reactions remains challenging in studies which involve reactions in the millisecond timescale, toxic chemicals, and/or anaerobic conditions. In this study, a three-dimensionally (3D) microfabricated microfluidic mixer platform that is compatible with time-resolved X-ray absorption and emission spectroscopy (XAS and XES, respectively) is presented. This platform, to initiate reactions and study their progression, mixes a high flow rate (0.50–1.5 ml min−1) sheath stream with a low-flow-rate (5–90 µl min−1) sample stream within a monolithic fused silica chip. The chip geometry enables hydrodynamic focusing of the sample stream in 3D and sample widths as small as 5 µm. The chip is also connected to a polyimide capillary downstream to enable sample stream deceleration, expansion, and X-ray detection. In this capillary, sample widths of 50 µm are demonstrated. Further, convection–diffusion-reaction models of the mixer are presented. The models are experimentally validated using confocal epifluorescence microscopy and XAS/XES measurements of a ferricyanide and ascorbic acid reaction. The models additionally enable prediction of the residence time and residence time uncertainty of reactive species as well as mixing times. Residence times (from initiation of mixing to the point of X-ray detection) during sample stream expansion as small as 2.1 ± 0.3 ms are also demonstrated. Importantly, an exploration of the mixer operational space reveals a theoretical minimum mixing time of 0.91 ms. The proposed platform is applicable to the determination of the electronic structure of conventionally inaccessible reaction intermediates.

scholarly journals Biological treatment of drippers clogged by the use of treated domestic wastewater

2016 ◽  
Vol 20 (7) ◽  
pp. 595-599
Author(s):  
Danniely O. Costa ◽  
Rafael O. Batista ◽  
Hudson S. M. Vale ◽  
Amanda B. de Sousa ◽  
Solange A. G. Dombroski ◽  
...  
Keyword(s):  
Flow Rate ◽  
Biological Treatment ◽  
Statistical Difference ◽  
Domestic Wastewater ◽  
Rate Reduction ◽  
Relative Flow Rate ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Different Doses ◽  
Relative Flow

ABSTRACT This study aimed to recover the flow rate of clogged drippers with different doses and permanence times of the product MaxBio. The experiment was conducted in a completely randomized design in split-split-plot scheme, where the plots corresponded to product doses (80, 160, 240 and 320 mg L-1), subplots to the applied treatments (T1 - without biological treatment, T2 - 1st application of the product, and T3 - 2nd application of the product) and in sub-subplots the types of emitters (Plastro Hydrodrip Super, Netafim Tiran and Netafim PCJ-CNJ), with three replicates. The four irrigation units operated with treated domestic wastewater for 400 h in order to clog the emitters. After applying the product MaxBio, the flow rate of the drippers and the relative flow rate reduction were determined to express the unclogging levels. There was significant effect of T2 and T3 on the recovery of the flow rate of the drippers, while for the reduction of relative flow rate, the doses of the product did not show statistical difference regarding the types of drippers.

scholarly journals EFFECT OF OXIDANT RELATIVE FLOW RATE ON OBTAINING RAW MATERIAL FOR PULVERIZED COAL PRODUCTION FROM HIGH-SULFURIC LOW GRADE COAL

2017 ◽  
Vol 11 (2) ◽  
pp. 236-241 ◽  
Author(s):  
Mariіa Shved ◽  
◽  
Serhiy Pyshyev ◽  
Yuriy Prysiazhnyi ◽  
◽  
...  
Keyword(s):  
Flow Rate ◽  
Pulverized Coal ◽  
Raw Material ◽  
Low Grade ◽  
Coal Production ◽  
Relative Flow Rate ◽  
Relative Flow

scholarly journals The Improvement of the Mathematical Model of the Work Process of Borehole Ejection Systems

2020 ◽  
pp. 36-44
Author(s):  
Ye. І. Kryzhanivskyi ◽  
D. О. Panevnyk
Keyword(s):  
Mathematical Models ◽  
Flow Rate ◽  
Hydraulic System ◽  
Pressure Ratio ◽  
Energy Performance ◽  
Maximum Efficiency ◽  
Relative Pressure ◽  
Jet Pump ◽  
Relative Flow Rate ◽  
Relative Flow

The article presents the sequence of deriving the equations which characterize the hydraulic system of jet pumps that carry out suction and injection-suction bottom flushing while drilling the production wells. The nature of flow distribution in the borehole ejection systems of the suction and injection-suction types is analyzed. The analysis is carried out taking into consideration the peculiarities of the calculation of branched hydraulic systems. While studying the movement of the flows in the bottom circulation circuits, the authors take into account the equation of the balance of the flow-rate at nodal points and hydraulic losses in parallel parts of the system. The developed mathematical models are based on the study of the changes in hydrodynamic pressures which occur in characteristic sections of a jet pump. The pressure ratio of mixed, injected and operating flows is presented as non-dimensional relative pressure of the ejection system. The dependence of non-dimensional relative pressure on the relative flow rate determines the characteristic of the hydraulic system of a jet pump. The relative flow rate or injection ratio of a jet pump is defined as the ratio of flow rate to injected and work flow. While analyzing the developed mathematical models, the authors specify the relation between the design and operating parameters of a jet pump. These parameters ensure its operation with maximum efficiency. Based on the study of the influence of the correlation between the diameters of the jet pump nozzles and the flushing system of the bit, the authors specify parameters which are optimal in terms of energy performance. The article provides the example of graphical determination of the operating point of a pumping unit. This determination is based on a simultaneous solution of the equations of the characteristics of the jet pump and the hydraulic system in which it operates. The improved techniques allow to predict the mode parameters of ejection systems and to determine the size of flowing part of the jet pump. They provide maximum energy performance of its workflow.

Challenges Associated With Replicating Rotor Blade Deposition in a Non-Rotating Annular Cascade

2019 ◽  
Author(s):  
Christopher P. Bowen ◽  
Ali Ameri ◽  
Jeffrey P. Bons
Keyword(s):  
Flow Field ◽  
Incidence Angle ◽  
Rotor Blades ◽  
Absolute Pressure ◽  
Inlet Section ◽  
Turbine Engine ◽  
Model Flow ◽  
Annular Cascade ◽  
The Impact ◽  
Relative Flow

Abstract A computational analysis is performed to determine if particulate impact events on the external surfaces of gas turbine engine rotor blades can be faithfully replicated in an experimental rotor cascade. The General Electric (GE) Energy Efficient Engine (E3) first-stage turbine flow-field at cruise conditions is first solved using a steady state explicit mixing plane approach with non-reflecting treatment. To model flow in the cascade, a single E3 rotor periodic domain is then constructed with an inlet section matching the relative flow incidence angle from the mixing plane calculation. The mass-averaged relative flow conditions at the inlet and outlet of the mixing plane rotor section are imposed on the cascade boundaries and a steady solution is found. Particles with diameters ranging from 1 to 25 μm are tracked through each fluid domain using a Lagrangian approach, and the OSU Deposition Model is implemented to dictate the sticking and rebounding action when particles interact with solid surfaces. The impact locations on the blade are compared between the rotating (mixing plane) and stationary (cascade) cases. It is discovered that both the locations and parameters of the particle impacts in the cascade vary significantly from the engine environment. For smaller particles, this deviation is credited to a stronger upstream influence of the blade on the cascade flow-field. As particle size increases, this effect tapers off, and the differences in deposition are instead driven by the interaction of the full-stage vane with the particles. The lack of a vane in the cascade causes drastically different particle inlet vectors over the rotor than are seen in the engine setting. The radial differences of particle impact locations are explored, and the role that absolute pressure plays is considered.

The Impact of the Multiple Reference Frame Interface on Modelling the Interaction Between IGVs and the Impeller in Turbocharger Compressors

2017 ◽  
Author(s):  
Xiangjun Li ◽  
Stephen Spence
Keyword(s):  
Flow Field ◽  
Mass Flow Rate ◽  
Reference Frame ◽  
Mass Flow ◽  
Flow Rate ◽  
Rotational Speed ◽  
Axial Position ◽  
High Rotational Speed ◽  
The Impact ◽  
Multiple Reference

In this paper numerical investigations are presented of how the axial position of the multiple reference frame (MRF) stator-rotor interface between the inlet guide vanes (IGVs) and the impeller would influence the predicted flow field for a turbocharger centrifugal compressor when simulated by the steady RANS method. In the first step, a total of three different axial positions of the MRF IGV-impeller interface were considered and compared with the results of an unsteady simulation to evaluate their accuracy. The results showed that the choice of the MRF interface location significantly influenced the predicted overall performance. At the lower rotational speed, the peak efficiency varied by 1.3% and the corresponding total pressure ratio varied by 0.022. At the high rotational speed, the different axial locations of the MRF interface varied the predicted choke point by 0.012 normalized mass flow rate. The mass flow rate of the near surge (NS) point was over estimated at both the high and low rotational speed by at least 0.038 normalized mass flow rate. Consideration of the flow field suggested that the MRF interface between the IGV and the impeller should be placed towards the upstream side of the available region to avoid being unphysically influenced by its interaction with the non-uniform pressure in the downstream subsonic flow field and to enable a more accurate prediction of the extent of the inducer shock in transonic operating situations. Based on this understanding, a further improvement was made for the setting of the MRF interface by employing a polyline interface. This achieved a more accurate numerical result for the NS operating point at low rotational speeds. The position of the MRF interface for modelling IGVs in a turbocharger compressor should be suitably chosen according to the objectives of the numerical study.

scholarly journals Using a Microfluidics System to Reproducibly Synthesize Protein Nanoparticles: Factors Contributing to Size, Homogeneity, and Stability

Processes ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 290 ◽  
Author(s):  
Courtney van Ballegooie ◽  
Alice Man ◽  
Irene Andreu ◽  
Byron D. Gates ◽  
Donald Yapp
Keyword(s):  
Flow Rate ◽  
Base Material ◽  
Tween 80 ◽  
Sodium Caseinate ◽  
Total Flow ◽  
Total Flow Rate ◽  
Relative Flow Rate ◽  
Relative Flow

The synthesis of Zein nanoparticles (NPs) using conventional methods, such as emulsion solvent diffusion and emulsion solvent evaporation, is often unreliable in replicating particle size and polydispersity between batch-to-batch syntheses. We have systematically examined the parameters for reproducibly synthesizing Zein NPs using a Y-junction microfluidics chip with staggered herringbone micromixers. Our results indicate that the total flow rate of the fluidics system, relative flow rate of the aqueous and organic phase, concentration of the base material and solvent, and properties of the solvent influence the polydispersity and size of the NPs. Trends such as increasing the total flow rate and relative flow rate lead to a decrease in Zein NP size, while increasing the ethanol and Zein concentration lead to an increase in Zein NP size. The solvent property that was found to impact the size of the Zein NPs formed the most was their hydropathy. Solvents that had a hydropathy index most similar to that of Zein formed the smallest Zein NPs. Synthesis consistency was confirmed within and between sample batches. Stabilizing agents, such as sodium caseinate, Tween 80, and Pluronic F-68, were incorporated using the microfluidics system, necessary for in vitro and in vivo use, into Zein-based NPs.

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