Fluid Dynamic Study in a Femoral Artery Branch Casting of Man With Upstream Main Lumen Curvature for Steady Flow

1985 ◽  
Vol 107 (3) ◽  
pp. 240-248 ◽  
Author(s):  
M. R. Back ◽  
Y. I. Cho ◽  
L. H. Back
Keyword(s):  
Steady Flow ◽  
Fluid Transport ◽  
Fluid Dynamic ◽  
Three Dimensional ◽  
Flow Characteristics ◽  
Pressure Distributions ◽  
Flow Investigation ◽  
Measured Pressure

An in-vitro, steady flow investigation was conducted in a hollow, transparent vascular replica of the profunda femoris branch of man for a range of physiological flow conditions. The replica casting tested was obtained from a human cadaver and indicated some plaque formation along the main lumen and branch. The flow visualization observations and measured pressure distributions indicated the highly three-dimensional flow characteristics with arterial curvature and branching, and the important role of centrifugal effects in fluid transport mechanisms.

500 An in vitro steady flow model of mitral regurgitation by three-dimensional Doppler

1999 ◽  
Vol 1 ◽  
pp. S86-S86
Author(s):  
R DESIMONE ◽  
G GLOMBITZA ◽  
C VAHL ◽  
H MEINZER ◽  
S HAGL
Keyword(s):  
Mitral Regurgitation ◽  
Steady Flow ◽  
Flow Model ◽  
Three Dimensional

scholarly journals The Role of Pre-Clinical 3-Dimensional Models of Osteosarcoma

2020 ◽  
Vol 21 (15) ◽  
pp. 5499
Author(s):  
Hannah L. Smith ◽  
Stephen A. Beers ◽  
Juliet C. Gray ◽  
Janos M. Kanczler
Keyword(s):  
Three Dimensional ◽  
Chorioallantoic Membrane ◽  
3D Models ◽  
In Ovo ◽  
Future Directions ◽  
3 Dimensional ◽  
In Vivo Animal Models

Treatment for osteosarcoma (OS) has been largely unchanged for several decades, with typical therapies being a mixture of chemotherapy and surgery. Although therapeutic targets and products against cancer are being continually developed, only a limited number have proved therapeutically active in OS. Thus, the understanding of the OS microenvironment and its interactions are becoming more important in developing new therapies. Three-dimensional (3D) models are important tools in increasing our understanding of complex mechanisms and interactions, such as in OS. In this review, in vivo animal models, in vitro 3D models and in ovo chorioallantoic membrane (CAM) models, are evaluated and discussed as to their contribution in understanding the progressive nature of OS, and cancer research. We aim to provide insight and prospective future directions into the potential translation of 3D models in OS.

scholarly journals Osteogenic differentiation and mineralization in fibre-reinforced tubular scaffolds: theoretical study and experimental evidences

2012 ◽  
Vol 9 (74) ◽  
pp. 2201-2212 ◽  
Author(s):  
Vincenzo Guarino ◽  
Francesco Urciuolo ◽  
Marco A. Alvarez-Perez ◽  
Benedetto Mele ◽  
Paolo A. Netti ◽  
...  
Keyword(s):  
Fluid Transport ◽  
Mechanical Response ◽  
Fluid Dynamic ◽  
Human Mesenchymal Stem Cells ◽  
Filament Winding ◽  
Long Bone ◽  
Design Strategies ◽  
Dynamic Simulations ◽  
Scale Properties

The development of composite scaffolds with well-organized architecture and multi-scale properties (i.e. porosity, degradation) represents a valid approach for achieving a tissue-engineered construct capable of reproducing the medium- and long-term in vitro behaviour of hierarchically complex tissues such as spongy bone. To date, the implementation of scaffold design strategies able to summarize optimal scaffold architecture as well as intrinsic mechanical, chemical and fluid transport properties still remains a challenging issue. In this study, poly ɛ -caprolactone/polylactid acid (PCL/PLA) tubular devices (fibres of PLA in a PCL matrix) obtained by phase inversion/salt leaching and filament winding techniques were proposed as cell instructive scaffold for bone osteogenesis. Continuous fibres embedded in the polymeric matrix drastically improved the mechanical response as confirmed by compression elastic moduli, which vary from 0.214 ± 0.065 to 1.174 ± 0.143 MPa depending on the relative fibre/matrix and polymer/solvent ratios. Moreover, computational fluid dynamic simulations demonstrated the ability of composite structure to transfer hydrodynamic forces during in vitro culture, thus indicating the optimal flow rate conditions that, case by case, enables specific cellular events—i.e. osteoblast differentiation from human mesenchymal stem cells (hMSCs), mineralization, etc. Hence, we demonstrate that the hMSC differentiation preferentially occurs in the case of higher perfusion rates—over 0.05 ml min –1 —as confirmed by the expression of alkaline phosphate and osteocalcin markers. In particular, the highest osteopontin values and a massive mineral phase precipitation of bone-like phases detected in the case of intermediate flow rates (i.e. 0.05 ml min –1 ) allows us to identify the best condition to stimulate the bone extracellular matrix in-growth, in agreement with the hydrodynamic model prediction. All these results concur to prove the succesful use of tubular composite as temporary device for long bone treatment.

Three-Dimensional Simulation of In Vitro Angiogenesis: Effects of Extracellular Matrix Structure and Density

2010 ◽  
Author(s):  
Lowell Taylor Edgar ◽  
James E. Guilkey ◽  
Clayton J. Underwood ◽  
Brenda Baggett ◽  
Urs Utzinger ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Chemical Factors ◽  
Dimensional Simulation ◽  
Endothelial Growth Factor ◽  
In Vitro Angiogenesis

The process of angiogenesis is regulated by both chemical and mechanical signaling. While the role of chemical factors such as vascular endothelial growth factor (VEGF) during angiogenesis has been extensively studied, the influence of the mechanostructural environment on new vessel generation has received significantly less attention. During angiogenesis, endothelial cells in the existing vasculature detach and migrate out into the surrounding extracellular matrix (ECM), forming tubular structures that eventually mature into new blood vessels. This process is modulated by the structure and composition of the ECM [1]. The ECM is then remodeled by endothelial cells in the elongating neovessel tip, resulting in matrix condensation and changes in fiber orientation [2]. The mechanism as to how angiogenic vasculature and the ECM influence each other is poorly understood.

scholarly journals Overview of RNA G-quadruplex structures

2017 ◽  
Vol 63 (4) ◽  
Author(s):  
Magdalena Małgowska
Keyword(s):  
Three Dimensional ◽  
Telomere Maintenance ◽  
Structural Studies ◽  
Biological Functions ◽  
Cellular Processes ◽  
Novel Drugs ◽  
G Quadruplex ◽  
Conformational Diversity

G-quadruplexes are non-canonical secondary structures which may be formed by guanine rich sequences, both in vitro and in living cells. The number of biological functions assigned to these structural motifs has grown rapidly since the discovery of their involvement in the telomere maintenance. Knowledge of the three-dimensional structures of G-quadruplexes plays an important role in understanding their conformational diversity, physiological functions, and in the design of novel drugs targeting G-quadruplexes. For the last decades, structural studies have been mainly focused on the DNA G-quadruplexes. Their RNA counterparts gained an increased interest along with still-emerging recognition of the central role of RNA in multiple cellular processes. In this review we focus on structural properties of RNA G-quadruplexes, based on high-resolution structures, available in RCSB PDB data base and on structural models. In addition, we point out to the current challenges in this field of research.

scholarly journals Stereoscopic PIV of Steady Flow Through a Bileaflet Mechanical Heart Valve

2008 ◽  
Author(s):  
C. Hutchison ◽  
P. E. Sullivan ◽  
C. R. Ethier
Keyword(s):  
Steady Flow ◽  
Heart Valve ◽  
Heart Valves ◽  
Mechanical Heart Valve ◽  
Three Dimensional ◽  
Flow Characteristics ◽  
Shear Stresses ◽  
Stereoscopic Piv ◽  
Bileaflet Mechanical Heart Valve ◽  
Component Particle

Each year over 180,000 mechanical heart valves are implanted worldwide, with the bileaflet mechanical heart valve (BiMHV) accounting for approximately 85% of all valve replacements [1,2]. Although much improved from previous valve designs, aortic BiMHV design is far from ideal, and serious complications such as thromboembolism and hemolysis often result. Hemolysis and platelet activation are thought to be caused by turbulent Reynolds shear stresses in the flow [1]. Numerous previous studies have examined aortic BiMHV flow using LDA and two component Particle Image Velocimetry (PIV), and have shown the flow to be complex and three-dimensional [3,4]. Stereoscopic PIV (SPIV) can obtain all three velocity components on a flow plane, and hence has the potential to provide better understanding of three dimensional flow characteristics. The objective of the current study was to use SPIV to measure steady flow, including turbulence properties, downstream of a BiMHV in a modeled aorta. The resulting dataset will be useful for CFD model validation, and the intent is to make it publicly available.

scholarly journals Three-dimensional migration of macrophages requires Hck for podosome organization and extracellular matrix proteolysis

Blood ◽  
2010 ◽  
Vol 115 (7) ◽  
pp. 1444-1452 ◽  
Author(s):  
Céline Cougoule ◽  
Véronique Le Cabec ◽  
Renaud Poincloux ◽  
Talal Al Saati ◽  
Jean-Louis Mège ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Spatial Organization ◽  
Ectopic Expression ◽  
Three Dimensional ◽  
Matrix Metalloproteases ◽  
3 Dimensional ◽  
Normal Expression ◽  
Migration Of Macrophages

Abstract Tissue infiltration of phagocytes exacerbates several human pathologies including chronic inflammations or cancers. However, the mechanisms involved in macrophage migration through interstitial tissues are poorly understood. We investigated the role of Hck, a Src-family kinase involved in the organization of matrix adhesion and degradation structures called podosomes. In Hck−/− mice submitted to peritonitis, we found that macrophages accumulated in interstitial tissues and barely reached the peritoneal cavity. In vitro, 3-dimensional (3D) migration and matrix degradation abilities, 2 protease-dependent properties of bone marrow–derived macrophages (BMDMs), were affected in Hck−/− BMDMs. These macrophages formed few and undersized podosome rosettes and, consequently, had reduced matrix proteolysis operating underneath despite normal expression and activity of matrix metalloproteases. Finally, in fibroblasts unable to infiltrate matrix, ectopic expression of Hck provided the gain–of–3D migration function, which correlated positively with formation of podosome rosettes. In conclusion, spatial organization of podosomes as large rosettes, proteolytic degradation of extracellular matrix, and 3D migration appeared to be functionally linked and regulated by Hck in macrophages. Hck, as the first protein combining a phagocyte-limited expression with a role in 3D migration, could be a target for new anti-inflammatory and antitumor molecules.

Thermal Mixing Downstream of a 90-Degree T-Junction: Laminar and Turbulent Flows

2019 ◽  
Author(s):  
Bakhtier Farouk
Keyword(s):  
Turbulent Flows ◽  
Thermophysical Properties ◽  
Fluid Dynamic ◽  
Three Dimensional ◽  
Flow Characteristics ◽  
Inlet Temperature ◽  
Horizontal Pipe ◽  
Thermal Mixing ◽  
Large Eddy ◽  
Inlet Conditions

Abstract A three-dimensional time dependent computational fluid dynamic (CFD) study of laminar and turbulent thermal mixing of two flows entering a 90° T-junction pipe is presented. The two incoming flows (both liquids) in the T-junction enter the flow domain with different inlet velocities, and temperatures. Water flow is considered in both the horizontal pipe and the vertical pipe. Inlet temperature differences and temperature dependent thermophysical properties are considered. Large eddy simulations (LES) with sub-grid scale (SGS) modeling were considered for the simulation of the turbulent cases. The flow characteristics, and thermal mixing behaviors and detailed mixing structures were simulated, and they showed that thermal mixing of the two streams are closely affected by the inlet conditions of the two streams and the inlet thermophysical properties of the two streams.

Computational Analyses of an In-Vitro Aneurysm Model Based on Three-Dimensional Angiography With Comparison to Phase Contrast Magnetic Resonance Imaging and Dye Injection Studies

2010 ◽  
Author(s):  
Stephanie M. George ◽  
Pierre Watson ◽  
John N. Oshinski ◽  
Charles W. Kerber ◽  
Daniel Karolyi ◽  
...  
Keyword(s):  
High Speed ◽  
Phase Contrast ◽  
Cfd Simulation ◽  
Fluid Dynamic ◽  
Three Dimensional ◽  
Cerebral Aneurysms ◽  
Computational Fluid Dynamic Simulation ◽  
Poor Correlation ◽  
Aneurysm Model

Computational fluid dynamic simulation (CFD) is a valuable tool that has been used to understand some of the fundamental conditions of cerebrovascular flow. Current methods include anatomic modeling of cerebral aneurysms derived from vascular imaging such as MRA, CTA, and three-dimensional angiography. The input blood flow waveforms can be represented from either mathematical models or physiologic sampling of flow with phase contrast MR techniques or particle image velocimetry (1). While there has been general acceptance of the validity of computational fluid dynamics, some research suggests that there can be poor correlation between CFD flow calculations and directly measured flow (2). Therefore, the purpose of this study is to qualitatively compare flow patterns in a cerebral aneurysm model using data derived from three sources: (i) direct phase contrast MRA measurement in the model; (ii) CFD simulation using computer models created from three dimensional angiography, and (iii) previously published high speed injection dye studies.

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