Blood Freezing to Nearly Absolute Zero Temperature: −272.29°C
1981 ◽
Vol 103
(1)
◽
pp. 24-26
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Keyword(s):
Human erythrocytes were collected in ACD anticoagulant, glycerolized with an equal volume of 8.6 M glycerol, 8 percent glucose, 1 percent fructose, and 0.3 percent Na2 EDTA solution, sealed in glass ampules, and frozen to −272.29°C (0.86°K) in liquid helium under high vacuum. Biological assays performed after thawing and three-stage dialysis to remove the glycerol showed less than 1 percent hemolysis. Intracellular potassium, 2,3 DPG, and ATP were within the normal range.
1989 ◽
Vol 86
(15)
◽
pp. 5671-5671
◽
1939 ◽
Vol 43
(1)
◽
pp. 49-69
◽
2012 ◽
Vol 27
(01n03)
◽
pp. 1345032
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Keyword(s):