Bioprinting Endothelial Cells With Alginate for 3D Tissue Constructs

2009 ◽  
Vol 131 (11) ◽  
Author(s):  
Saif Khalil ◽  
Wei Sun
Keyword(s):  
Endothelial Cells ◽  
Cell Viability ◽  
Sodium Alginate ◽  
Calcium Chloride ◽  
Computer Aided Design ◽  
Biologically Active ◽  
Freeform Fabrication ◽  
Computer Aided ◽  
Cell Placement

Advanced solid freeform fabrication (SFF) techniques have been an interest for constructing tissue engineered polymeric scaffolds because of its repeatability and capability of high accuracy in fabrication resolution at the scaffold macro- and microscales. Among many important scaffold applications, hydrogel scaffolds have been utilized in tissue engineering as a technique to confide the desired proliferation of seeded cells in vitro and in vivo into its architecturally porous three-dimensional structures. Such fabrication techniques not only enable the reconstruction of scaffolds with accurate anatomical architectures but also enable the ability to incorporate bioactive species such as growth factors, proteins, and living cells. This paper presents a bioprinting system designed for the freeform fabrication of porous alginate scaffolds with encapsulated endothelial cells. The bioprinting fabrication system includes a multinozzle deposition system that utilizes SFF techniques and a computer-aided modeling system capable of creating heterogeneous tissue scaffolds. The manufacturing process is biologically compatible and is capable of functioning at room temperature and relatively low pressures to reduce the fluidic shear forces that could deteriorate biologically active species. The deposition system resolution is 10 μm in the three orthogonal directions XYZ and has minimum velocity of 100 μm/s. The ideal concentrations of sodium alginate and calcium chloride were investigated to determine a viable bioprinting process. The results indicated that the suitable fabrication parameters were 1.5% (w/v) sodium alginate and 0.5% (w/v) calcium chloride. Degradation studies via mechanical testing showed a decrease in the elastic modulus by 35% after 3 weeks. Cell viability studies were conducted on the cell encapsulated scaffolds for validating the bioprinting process and determining cell viability of 83%. This work exhibits the potential use of accurate cell placement for engineering complex tissue regeneration using computer-aided design systems.

scholarly journals DESIGN AND EVALUATION OF CONTROLLED-RELEASE OCULAR INSERTS OF BRIMONIDINE-TARTRATE AND TIMOLOL MALEATE

2016 ◽  
Vol 9 (1) ◽  
pp. 79 ◽  
Author(s):  
Preethi G. B. ◽  
Prashanth Kunal
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Sodium Alginate ◽  
Calcium Chloride ◽  
Release Kinetics ◽  
Water Soluble ◽  
Moisture Loss ◽  
Timolol Maleate ◽  
Brimonidine Tartrate

<p><strong>Objective: </strong>The current work was attempted to formulate and evaluate a controlled-release matrix-type ocular inserts containing a combination of brimonidine tartrate and timolol maleate, with a view to sustain the drug release in the cul-de-sac of the eye.<strong></strong></p><p><strong>Methods: </strong>Initially, the infrared studies were done to determine the drug–polymer interactions. Sodium alginate-loaded ocuserts were prepared by solvent casting technique. Varying the concentrations of polymer—sodium alginate, plasticizer—glycerine, and cross-linking agent—calcium chloride by keeping the drug concentration constant, made a total of nine formulations. These formulations were evaluated for its appearance, drug content, weight uniformity, thickness uniformity, percentage moisture loss, percentage moisture absorption, and <em>in vitro </em>release profile of the ocuserts. Finally, accelerated stability studies and the release kinetics were performed on the optimised formulation.<strong></strong></p><p><strong>Results: </strong>It was perceived that polymer, plasticizer, and calcium chloride had a significant influence on the drug release. The data obtained from the formulations showed that formulation—F9 was the optimised formulation, which exhibited better drug release. The release data of the optimised formulation tested on the kinetic models revealed that it exhibited first-order release kinetics. <strong></strong></p><p><strong>Conclusion: </strong>It can be concluded that a natural bioadhesive hydrophilic polymer such as sodium alginate can be used as a film former to load water soluble and hydrophilic drugs like brimonidine tartrate and timolol maleate. Among all formulations, F9 with 400 mg sodium alginate, 2% calcium chloride and 60 mg glycerin were found to be the most suitable insert in terms of appearance, ease of handling, thickness, <em>in vitro</em> drug release and stability.</p>

scholarly journals Accuracy of Digital Impression Taking Using Intraoral Scanner Versus the Conventional Technique

2021 ◽  
Author(s):  
Arash Zarbakhsh ◽  
Ezatollah Jalalian ◽  
Nazanin Samiei ◽  
Mohammad Hossein Mahgoli ◽  
Hadi Kaseb Ghane
Keyword(s):  
Standard Model ◽  
Conventional Method ◽  
Computer Aided Design ◽  
Conventional Technique ◽  
Digital Impression ◽  
Digital Method ◽  
Intraoral Scanner ◽  
The Standard Model ◽  
Computer Aided

Objectives: Intraoral scanners have shown promising results when used as an adjunct or alternative to conventional impression techniques. This study compared the accuracy of digital impression taking using an intraoral scanner versus the conventional technique. Materials and Methods: In this in-vitro experimental study, a typodont molar tooth was prepared as the standard model and scanned by TRIOS intraoral scanner. Ten digital impressions were fabricated as such and intraoral scans were sent to the manufacturers. In the conventional method, using addition silicone impression material, a stone die was fabricated. Using a computer-aided design/computer-aided manufacturing scanner, the die was scanned, and the data were transferred to the software. After the fabrication of frameworks, the replica technique was used. The replicas’ thickness (indicative of the gap between the framework and the model and the accuracy of impression taking) was 12 points. The data were analyzed using student's t-test. Results: The mean thickness of replicas (gap between the internal surface of frameworks and the standard model) at the three points in the buccal, lingual, mesial, and distal sections in the digital impression technique was lower than that in the conventional technique (P<0.0001). In other words, the accuracy of impressions taken by the digital method was significantly higher than those taken by the conventional method. Conclusion: Intraoral digital scanner had significantly higher accuracy than the conventional method in all points. Thus, the digital method can be reliably used as an adjunct or alternative to the conventional method to increase the accuracy of impression taking.

scholarly journals Kisspeptin-10 Inhibits Angiogenesis in Human Placental Vessels ex Vivo and Endothelial Cells in Vitro

Endocrinology ◽  
2010 ◽  
Vol 151 (12) ◽  
pp. 5927-5934 ◽  
Author(s):  
Thayalini Ramaesh ◽  
James J. Logie ◽  
Antonia K. Roseweir ◽  
Robert P. Millar ◽  
Brian R. Walker ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Vessels ◽  
Ex Vivo ◽  
Umbilical Vein ◽  
Biologically Active ◽  
Trophoblast Invasion ◽  
In Vitro Assays ◽  
Dependent Manner ◽  
Inhibition Of Proliferation

Recent studies suggest that kisspeptin (a neuropeptide central to the regulation of gonadotrophin secretion) has diverse roles in human physiology, including a putative role in implantation and placental function. Kisspeptin and its receptor are present in human blood vessels, where they mediate vasoconstriction, and kisspeptin is known to inhibit tumor metastasis and trophoblast invasion, both processes involving angiogenesis. We hypothesized that kisspeptin contributes to the regulation of angiogenesis in the reproductive system. The presence of the kisspeptin receptor was confirmed in human placental blood vessels and human umbilical vein endothelial cells (HUVEC) using immunochemistry. The ability of kisspeptin-10 (KP-10) (a shorter biologically active processed peptide) to inhibit angiogenesis was tested in explanted human placental arteries and HUVEC using complementary ex vivo and in vitro assays. KP-10 inhibited new vessel sprouting from placental arteries embedded in Matrigel and tube-like structure formation by HUVEC, in a concentration-dependent manner. KP-10 had no effect on HUVEC viability or apoptosis but induced concentration-dependent inhibition of proliferation and migration. In conclusion, KP-10 has antiangiogenic effects and, given its high expression in the placenta, may contribute to the regulation of angiogenesis in this tissue.

Review of Marginal Adaptation and Fracture Resistance of Computer Aided Design/Computer Aided Manufacturer (CAD‐CAM) Fabricated Endo-crowns

2021 ◽  
pp. 196-208
Author(s):  
Abdulrahman Alhaddad ◽  
Samar Abuzinadah ◽  
Abdullah Al-Otaibi ◽  
Abrar Alotaibi ◽  
Mohsen Alfkih ◽  
...  
Keyword(s):  
Computer Aided Design ◽  
Fracture Resistance ◽  
Systemic Review ◽  
Marginal Adaptation ◽  
Marginal Gap ◽  
Computer Aided ◽  
Cad Cam ◽  
Electronic Database Search ◽  
Cause Of Failure

Background: Zirconia-based restorations have become more popular in dentistry during the last two decades. Patients choose metal-free restorations, preferring materials with similar attributes to natural teeth and similar light scattering characteristics, resulting in a nice esthetic appearance. Restoring a root canal treated teeth is one of the hot topics today. endo crown materials can be either; feldspathic, glass-ceramic, monolithic hybrid ceramic or composite material. Considering the marginal gap of endocrown, an important cause of failure of treatment, the current study evaluated the marginal gap of CAD‐CAM concocted endo-crowns. Materials and Methods: This research is an analysis systemic review study was conducted between January 2020 and October 2021. We followed the PRISMA principles and recorded this systematic review using the PROSPERO database to find and identify published literature related to the marginal adaptation of CAD-CAM-fabricated endocrown. The search will include all relevant articles through the end of 2021. Finally, 24 papers on marginal clearance and fracture resistance in coronary arteries were reviewed. Results: The electronic database search yielded 98 studies that were relevant. After cross-referencing, further seven studies were added. After a full-text analysis and duplicate reduction, 74 of the 98 articles were eliminated. 5 clinical (prospective) studies, 19 in vitro studies were found. Conclusion: This analysis of the recent literature on the marginal seating integrity and fracture resistance of CAD/CAM made-up endo-crowns showed that the endo-crown had superior marginal seating integrity than classical full crown. CAM/CAM showed statistically significant higher mean fracture resistance than MAD/MAM.

scholarly journals Single blinded in-vitro study comparing microleakage between CAD/CAM crowns milled out of feldspathic ceramic and resin nano ceramic, cemented with three resin cements

2019 ◽  
Vol 18 (4) ◽  
pp. 764-772
Author(s):  
Asa Yazdani Fard ◽  
Zuryati Ab Ghani ◽  
Zaihan Ariffin ◽  
Dasmawati Mohamad
Keyword(s):  
Computer Aided Design ◽  
Medical Science ◽  
In Vitro Study ◽  
Resin Cement ◽  
Blue Dye ◽  
Resin Cements ◽  
Etch And Rinse ◽  
Computer Aided ◽  
Cad Cam

Background: Studies on microleakage of Computer-Aided Design/Computer-Aided Manufacturing (CAD/CAM) crowns are abundant. However many of them are inconclusive, especially those using self adhesive cements. Aims: To compare the microleakage between CAD/CAM crowns milled out of feldspathic ceramic and resin nano ceramics, cemented with three resin cements. Materials and Methods: Crown preparation was made on 54 extracted human premolars. Impressions were captured optically using CEREC 3D machine intraoral camera, and crowns were milled from feldspathic ceramic (CEREC® Blocs PC, VITA) and resin nano ceramic (Lava™ Ultimate CAD/CAM Restorative, 3M ESPE) blocks. The crowns were then cemented with three cements (n = 9); RelyX™ U200 Self-Adhesive Resin Cement (3M ESPE); NX3 Nexus ® cement with two-step etch-and-rinse adhesive (Kerr Corporation) or three/multistep etch-and-rinse resin cement, Variolink® II/Syntac Classic (Ivoclar Vivadent). The specimens were kept in water for 24 hours, thermocycled, and then soaked in methylene blue dye for 24 hours, before being sectioned mesiodistally. Microleakage was assessed using a fivepoint scale using stereomicroscope. Statistical analysis of the data was carried out using ONEWay ANOVA. Results: CEREC® Blocs PC crowns showed significantly less microleakage (p< 0.001) compared to Lava™ Ultimate. RelyX™ U200 showed significantly lower microleakage (p< 0.001) compared to other cements. Combination of Lava™ Ultimate crown cemented with RelyX™ U200 showed the least microleakage (p< 0.001). Conclusions: The microleakage scores were affected by the types of crown and cements. Bangladesh Journal of Medical Science Vol.18(4) 2019 p.764-772

scholarly journals Estrogen-Receptor-Mediated Protection of Cerebral Endothelial Cell Viability and Mitochondrial Function after Ischemic Insult in vitro

2009 ◽  
Vol 30 (3) ◽  
pp. 545-554 ◽  
Author(s):  
Jiabin Guo ◽  
Diana N Krause ◽  
James Horne ◽  
John H Weiss ◽  
Xuejun Li ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Estrogen Receptor ◽  
Endothelial Cell ◽  
Cell Viability ◽  
Mitochondrial Function ◽  
Protective Effects ◽  
Brain Endothelial Cells ◽  
Ischemic Insult ◽  
Mitochondrial Superoxide

Protective effects of estrogen against experimental stroke and neuronal ischemic insult are well-documented, but it is not known whether estrogen prevents ischemic injury to brain endothelium, a key component of the neurovascular unit. Increasing evidence indicates that estrogen exerts protective effects through mitochondrial mechanisms. We previously found 17β-estradiol (E2) to improve mitochondrial efficiency and reduce mitochondrial superoxide in brain blood vessels and endothelial cells. Thus we hypothesized E2 will preserve mitochondrial function and protect brain endothelial cells against ischemic damage. To test this, an in vitro ischemic model, oxygen-glucose deprivation (OGD)/reperfusion, was applied to immortalized mouse brain endothelial cells (bEnd.3). OGD/reperfusion-induced cell death was prevented by long-term (24, 48 h), but not short-term (0.5, 12 h), pretreatment with 10 nmol/L E2. Protective effects of E2 on endothelial cell viability were mimicked by an estrogen-receptor (ER) agonist selective for ERα (PPT), but not by one selective for ERβ (DPN). In addition, E2 significantly decreased mitochondrial superoxide and preserved mitochondrial membrane potential and ATP levels in early stages of OGD/reperfusion. All of the E2 effects were blocked by the ER antagonist, ICI-182,780. These findings indicate that E2 can preserve endothelial mitochondrial function and provide protection against ischemic injury through ER-mediated mechanisms.

scholarly journals Application Solid Laser-Sintered or Machined Ti6Al4V Alloy in Manufacturing of Dental Implants and Dental Prosthetic Restorations According to Dentistry 4.0 Concept

Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 664 ◽  
Author(s):  
Leszek A. Dobrzański ◽  
Lech B. Dobrzański ◽  
Anna Achtelik-Franczak ◽  
Joanna Dobrzańska
Keyword(s):  
Dental Implants ◽  
Computer Aided Design ◽  
Bending Strength ◽  
Ti6al4v Alloy ◽  
Cnc Machining ◽  
Live Cells ◽  
Computer Aided ◽  
Aided Design ◽  
The Impact

This paper presents a comparison of the impact of milling technology in the computer numerically controlled (CNC) machining centre and selective laser sintering (SLS) and on the structure and properties of solid Ti6Al4V alloy. It has been shown that even small changes in technological conditions in the SLS manufacturing variant significantly affect changes from two to nearly two and a half times in tensile and bending strengths. Both the tensile and bending strength obtained in the most favourable manufacturing variant by the SLS method is over 25% higher than in the case of cast materials subsequently processed by milling. Plug-and-play SLS conditions provide about 60% of the possibilities. Structural, tribological and electrochemical tests were carried out. In vitro biological tests using osteoblasts confirm the good tendency for the proliferation of live cells on the substrate manufactured under the most favourable SLS conditions. The use of SLS additive technology for the manufacturing of dental implants and abutments made of Ti6Al4V alloy in combination with the digitisation of dental diagnostics and computer-aided design and manufacture of computer-aided design/manufacturing (CAD/CAM) following the idea of Dentistry 4.0 is the best choice of technology for manufacturing of prosthetic and implant devices used in dentistry.

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