Measuring Collagen Fiber Orientation: A Two-Dimensional Quantitative Macroscopic Technique

1998 ◽  
Vol 120 (4) ◽  
pp. 537-540 ◽  
Author(s):  
J. P. Dickey ◽  
B. R. Hewlett ◽  
G. A. Dumas ◽  
D. A. Bednar
Keyword(s):  
Fiber Orientation ◽  
Collagen Fiber ◽  
Polarized Light ◽  
Design Evaluation ◽  
Two Dimensional ◽  
Orientation Data ◽  
Collagen Fiber Orientation ◽  
System Validation ◽  
Pixel Brightness ◽  
New System

This paper describes the design, evaluation, and application of a new system for quantifying two-dimensional collagen fiber orientation in soft tissue. Series of transmitted polarized light images were collected using a custom-designed macroscope. Combined analysis of pixel brightness, and hue from images collected with a compensator plate, permitted the assignment of each pixel into the appropriate orientation band. Experiments were performed to quantify the linearity and noise of the system. Validation was performed on a specimen composed of strain-birefringent plastic strips at various orientations. Preliminary collagen fiber orientation data is presented from a tendon specimen. This study demonstrates the utility of this approach for studying collagen fiber orientation across large areas.

Circularly polarized light standards for investigations of collagen fiber orientation in bone

2003 ◽  
Vol 274B (1) ◽  
pp. 157-168 ◽  
Author(s):  
Timothy G. Bromage ◽  
Haviva M. Goldman ◽  
Shannon C. McFarlin ◽  
Johanna Warshaw ◽  
Alan Boyde ◽  
...  
Keyword(s):  
Fiber Orientation ◽  
Collagen Fiber ◽  
Polarized Light ◽  
Circularly Polarized Light ◽  
Circularly Polarized ◽  
Collagen Fiber Orientation

A Review of the Collagen Orientation in the Articular Cartilage

Cartilage ◽  
2021 ◽  
pp. 194760352098877
Author(s):  
Roy D. Bloebaum ◽  
Andrew S. Wilson ◽  
William N. Martin
Keyword(s):  
Surface Layer ◽  
Articular Cartilage ◽  
Fiber Orientation ◽  
Collagen Fiber ◽  
Articular Surface ◽  
Imaging Techniques ◽  
Collagen Fibers ◽  
Deep Zone ◽  
Collagen Fiber Orientation ◽  
Critical Point Drying

Objective There has been a debate as to the alignment of the collagen fibers. Using a hand lens, Sir William Hunter demonstrated that the collagen fibers ran perpendicular and later aspects were supported by Benninghoff. Despite these 2 historical studies, modern technology has conflicting data on the collagen alignment. Design Ten mature New Zealand rabbits were used to obtain 40 condyle specimens. The specimens were passed through ascending grades of alcohol, subjected to critical point drying (CPD), and viewed in the scanning electron microscope. Specimens revealed splits from the dehydration process. When observing the fibers exposed within the opening of the splits, parallel fibers were observed to run in a radial direction, normal to the surface of the articular cartilage, radiating from the deep zone and arcading as they approach the surface layer. After these observations, the same samples were mechanically fractured and damaged by scalpel. Results The splits in the articular surface created deep fissures, exposing parallel bundles of collagen fibers, radiating from the deep zone and arcading as they approach the surface layer. On higher magnification, individual fibers were observed to run parallel to one another, traversing radially toward the surface of the articular cartilage and arcading. Mechanical fracturing and scalpel damage induced on the same specimens with the splits showed randomly oriented fibers. Conclusion Collagen fiber orientation corroborates aspects of Hunter’s findings and compliments Benninghoff. Investigators must be aware of the limits of their processing and imaging techniques in order to interpret collagen fiber orientation in cartilage.

scholarly journals Collagen I membranes for tendon repair: Effect of collagen fiber orientation on cell behavior

2009 ◽  
Vol 27 (6) ◽  
pp. 826-832 ◽  
Author(s):  
Antonio Gigante ◽  
Eugenio Cesari ◽  
Alberto Busilacchi ◽  
Sandra Manzotti ◽  
Kyriaki Kyriakidou ◽  
...  
Keyword(s):  
Fiber Orientation ◽  
Collagen Fiber ◽  
Tendon Repair ◽  
Collagen I ◽  
Cell Behavior ◽  
Collagen Fiber Orientation

scholarly journals How the nanoscale composition and collagen fiber orientation affect osteonal mechanical competence

Bone Reports ◽  
2020 ◽  
Vol 13 ◽  
pp. 100673
Author(s):  
Kilian Stockhausen ◽  
Felix Schmidt ◽  
Mahan Qwamizadeh ◽  
Eva Wölfel ◽  
Haniyeh Hemmatian ◽  
...  
Keyword(s):  
Fiber Orientation ◽  
Collagen Fiber ◽  
Collagen Fiber Orientation

Collagen fiber orientation at the tendon to bone insertion and its influence on stress concentrations

2006 ◽  
Vol 39 (10) ◽  
pp. 1842-1851 ◽  
Author(s):  
Stavros Thomopoulos ◽  
Juan P. Marquez ◽  
Bradley Weinberger ◽  
Victor Birman ◽  
Guy M. Genin
Keyword(s):  
Fiber Orientation ◽  
Collagen Fiber ◽  
Stress Concentrations ◽  
Collagen Fiber Orientation

scholarly journals Quantitative Mapping of Collagen Fiber Orientation in Non-glaucoma and Glaucoma Posterior Human Sclerae

2012 ◽  
Vol 53 (9) ◽  
pp. 5258 ◽  
Author(s):  
Jacek K. Pijanka ◽  
Baptiste Coudrillier ◽  
Kimberly Ziegler ◽  
Thomas Sorensen ◽  
Keith M. Meek ◽  
...  
Keyword(s):  
Fiber Orientation ◽  
Collagen Fiber ◽  
Collagen Fiber Orientation ◽  
Quantitative Mapping
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