Turbulent Flow Evaluation of the Venous Needle During Hemodialysis

2005 ◽  
Vol 127 (7) ◽  
pp. 1141-1146 ◽  
Author(s):  
Sunil Unnikrishnan ◽  
Thanh N. Huynh ◽  
B. C. Brott ◽  
Y. Ito ◽  
C. H. Cheng ◽  
...  
Keyword(s):  
Graft Failure ◽  
Venous Anastomosis ◽  
Graft Stenosis ◽  
Graft Flow ◽  
Endothelial Denudation ◽  
Improved Design ◽  
Wall Injury ◽  
Av Graft

Arteriovenous (AV) grafts and fistulas used for hemodialysis frequently develop intimal hyperplasia (IH) at the venous anastomosis of the graft, leading to flow-limiting stenosis, and ultimately to graft failure due to thrombosis. Although the high AV access blood flow has been implicated in the pathogenesis of graft stenosis, the potential role of needle turbulence during hemodialysis is relatively unexplored. High turbulent stresses from the needle jet that reach the venous anastomosis may contribute to endothelial denudation and vessel wall injury. This may trigger the molecular and cellular cascade involving platelet activation and IH, leading to eventual graft failure. In an in-vitro graft/needle model dye injection flow visualization was used for qualitative study of flow patterns, whereas laser Doppler velocimetry was used to compare the levels of turbulence at the venous anastomosis in the presence and absence of a venous needle jet. Considerably higher turbulence was observed downstream of the venous needle, in comparison to graft flow alone without the needle. While turbulent RMS remained around 0.1m∕s for the graft flow alone, turbulent RMS fluctuations downstream of the needle soared to 0.4–0.7m∕s at 2 cm from the tip of the needle and maintained values higher than 0.1m∕s up to 7–8 cm downstream. Turbulent intensities were 5–6 times greater in the presence of the needle, in comparison with graft flow alone. Since hemodialysis patients are exposed to needle turbulence for four hours three times a week, the role of post-venous needle turbulence may be important in the pathogenesis of AV graft complications. A better understanding of the role of needle turbulence in the mechanisms of AV graft failure may lead to improved design of AV grafts and venous needles associated with reduced turbulence, and to pharmacological interventions that attenuate IH and graft failure resulting from turbulence.

NUMERICAL SIMULATION OF THE EFFECT OF VENOUS NEEDLE'S FLOW ON FLOW PARAMETERS OF HEMODIALYSIS GRAFT AND ITS HEMODYNAMIC CONSEQUENCES

2014 ◽  
Vol 26 (05) ◽  
pp. 1450057
Author(s):  
H. Niroomand-Oscuii ◽  
N. Meghdadi
Keyword(s):  
Shear Stress ◽  
Graft Failure ◽  
Cell Alignment ◽  
Venous Anastomosis ◽  
Graft Stenosis ◽  
Graft Flow ◽  
Inlet Conditions ◽  
The Individual ◽  
Stage Renal Disease ◽  
Hemodialysis Graft

Arterio-Venous (AV) grafts which are used for hemodialysis, frequently develop intimal hyperplasia (IH) in venous anastomosis and ultimately leads to graft failure which is the major cause of morbidity and mortality in patients with the end stage renal disease (ESRD). Although the high AV access blood flow has been implicated in the pathogenesis of graft stenosis, the potential role of needle turbulence during hemodialysis is relatively unexplored. In the current study, a numerical survey is carried out to investigate the effect of venous needle's flow on hemodynamic parameters of the flow inside a hemodialysis graft. Two cases include the individual graft flow and the graft flow combined with a needle flow which both of them are evaluated and their results are compared to the cell morphology are represented in this research. Blood is considered as an incompressible Newtonian fluid with density of 1050 [Kg/(m3)] and dynamic viscosity of 0.0035 [Pa.s] and the graft was considered as a rigid vessel due to its low compliance. Flow rates were considered 500 and 100 [mL/min] as inlet conditions of the graft and the needle, respectively. Results show that the presence of the needle's flow causes to increase the amount of shear stress and nonuniformity of the shear stress distribution on the graft wall, where the cell alignment is lost in the presence of needle flow. These findings suggest the presence of potential relationship between venous needle flow during hemodialysis and endothelial dysfunction which may lead to vascular injury and graft failure.

Role of Coronary Graft Flow Measurement During Reoperations for Early Graft Failure After Off-Pump Coronary Revascularization

1999 ◽  
Vol 14 (5) ◽  
pp. 342-347 ◽  
Author(s):  
Marco Ricci ◽  
Hratch L. Karamanoukian ◽  
Tomas A. Salerno ◽  
Giuseppe D'Ancona ◽  
Jacob Bergsland
Keyword(s):  
Graft Failure ◽  
Flow Measurement ◽  
Coronary Revascularization ◽  
Off Pump ◽  
Graft Flow ◽  
Early Graft ◽  
Early Graft Failure

Role of Coronary Graft Flow Measurement During Reoperations for Early Graft Failure After Off-Pump Coronary Revascularization

1985 ◽  
Vol 2 (5) ◽  
pp. 342-347
Author(s):  
Marco Ricci ◽  
Hratch L. Karamanoukian ◽  
Tomas A. Salerno ◽  
Giuseppe D'Ancona ◽  
Jacob Bergsland
Keyword(s):  
Graft Failure ◽  
Flow Measurement ◽  
Coronary Revascularization ◽  
Off Pump ◽  
Graft Flow ◽  
Early Graft ◽  
Early Graft Failure

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

An integrated screening method for evaluating the pulmonary toxicity of inhaled particulates: Role of microscopic techniques in assessing pulmonary macrophage clearance functions

1990 ◽  
Vol 48 (3) ◽  
pp. 826-827
Author(s):  
David B. Warheit ◽  
Lena Achinko ◽  
Mark A. Hartsky
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pulmonary Toxicity ◽  
Screening Method ◽  
Pulmonary Response ◽  
Inhaled Particles ◽  
Cytochemical Analysis ◽  
Pulmonary Macrophages ◽  
Integrated Screening

There is a great need for the development of a rapid and reliable bioassay to evaluate the pulmonary toxicity of inhaled particles. A number of methods have been proposed, including lung clearance studies, bronchoalveolar lavage analysis, and in vitro cytotoxicity tests. These methods are often limited in scope inasmuch as they measure only one dimension of the pulmonary response to inhaled, instilled or incubated dusts. Accordingly, a comprehensive approach to lung toxicity studies has been developed.To validate the method, rats were exposed for 6 hours or 3 days to various concentrations of either aerosolized alpha quartz silica (Si) or carbonyl iron (CI) particles. Cells and fluids from groups of sham and dust-exposed animals were recovered by bronchoalveolar lavage (BAL). Alkaline phosphatase, LDH and protein values were measured in BAL fluids at several time points postexposure. Cells were counted and evaluated for viability, as well as differential and cytochemical analysis. In addition, pulmonary macrophages (PM) were cultured and studied for morphology, chemotaxis, and phagocytosis by scanning electron microscopy.

The Role of Polyphenols in the Modulation of Plasma Non-Enzymatic Antioxidant Capacity (NEAC)

2012 ◽  
Vol 82 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Mauro Serafini ◽  
Giuseppa Morabito
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Intervention ◽  
Ex Vivo ◽  
The Body ◽  
Dietary Polyphenols ◽  
Enzymatic Antioxidant ◽  
Endogenous Antioxidant

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.

Anti-obesity role of Aster glehni extract: in vivo and in vitro effects

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
HM Lee ◽  
TG Ahn ◽  
CW Kim ◽  
HJ An
Keyword(s):  
In Vitro Effects

Role of the 807 C/T Polymorphism of the α2 Gene in Platelet GP Ia Collagen Receptor Expression and Function

1999 ◽  
Vol 81 (06) ◽  
pp. 951-956 ◽  
Author(s):  
J. Corral ◽  
R. González-Conejero ◽  
J. Rivera ◽  
F. Ortuño ◽  
P. Aparicio ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Cell Types ◽  
Receptor Expression ◽  
Case Control Studies ◽  
Platelet Surface ◽  
Vitro Analysis ◽  
And Function ◽  
In Vitro Analysis

SummaryThe variability of the platelet GP Ia/IIa density has been associated with the 807 C/T polymorphism (Phe 224) of the GP Ia gene in American Caucasian population. We have investigated the genotype and allelic frequencies of this polymorphism in Spanish Caucasians. The T allele was found in 35% of the 284 blood donors analyzed. We confirmed in 159 healthy subjects a significant association between the 807 C/T polymorphism and the platelet GP Ia density. The T allele correlated with high number of GP Ia molecules on platelet surface. In addition, we observed a similar association of this polymorphism with the expression of this protein in other blood cell types. The platelet responsiveness to collagen was determined by “in vitro” analysis of the platelet activation and aggregation response. We found no significant differences in these functional platelet parameters according to the 807 C/T genotype. Finally, results from 3 case/control studies involving 302 consecutive patients (101 with coronary heart disease, 104 with cerebrovascular disease and 97 with deep venous thrombosis) determined that the 807 C/T polymorphism of the GP Ia gene does not represent a risk factor for arterial or venous thrombosis.

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