Trabecular Shear Stresses Predict In Vivo Linear Microcrack Density but not Diffuse Damage in Human Vertebral Cancellous Bone

2003 ◽  
Vol 31 (6) ◽  
pp. 726-732 ◽  
Author(s):  
Yener N. Yeni ◽  
Fu J. Hou ◽  
Traci Ciarelli ◽  
Deepak Vashishth ◽  
David P. Fyhrie
Keyword(s):  
Cancellous Bone ◽  
Shear Stresses ◽  
Microcrack Density ◽  
Vertebral Cancellous Bone

Induction of in Vivo Ectopic Hematopoiesis by a Three-Dimensional Structured Extracellular Matrix Derived from Decellularized Cancellous Bone

2019 ◽  
Vol 5 (11) ◽  
pp. 5669-5680 ◽  
Author(s):  
Naoko Nakamura ◽  
Tsuyoshi Kimura ◽  
Kwangwoo Nam ◽  
Toshiya Fujisato ◽  
Hiroo Iwata ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cancellous Bone ◽  
Three Dimensional

scholarly journals Ibudilast Mitigates Delayed Bone Healing Caused by Lipopolysaccharide by Altering Osteoblast and Osteoclast Activity

2021 ◽  
Vol 22 (3) ◽  
pp. 1169
Author(s):  
Yuhan Chang ◽  
Chih-Chien Hu ◽  
Ying-Yu Wu ◽  
Steve W. N. Ueng ◽  
Chih-Hsiang Chang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bone Formation ◽  
Cancellous Bone ◽  
Bone Healing ◽  
Bone Bridge ◽  
Cell Wall Components ◽  
Osteoclast Activity ◽  
Wall Components

Bacterial infection in orthopedic surgery is challenging because cell wall components released after bactericidal treatment can alter osteoblast and osteoclast activity and impair fracture stability. However, the precise effects and mechanisms whereby cell wall components impair bone healing are unclear. In this study, we characterized the effects of lipopolysaccharide (LPS) on bone healing and osteoclast and osteoblast activity in vitro and in vivo and evaluated the effects of ibudilast, an antagonist of toll-like receptor 4 (TLR4), on LPS-induced changes. In particular, micro-computed tomography was used to reconstruct femoral morphology and analyze callus bone content in a femoral defect mouse model. In the sham-treated group, significant bone bridge and cancellous bone formation were observed after surgery, however, LPS treatment delayed bone bridge and cancellous bone formation. LPS inhibited osteogenic factor-induced MC3T3-E1 cell differentiation, alkaline phosphatase (ALP) levels, calcium deposition, and osteopontin secretion and increased the activity of osteoclast-associated molecules, including cathepsin K and tartrate-resistant acid phosphatase in vitro. Finally, ibudilast blocked the LPS-induced inhibition of osteoblast activation and activation of osteoclast in vitro and attenuated LPS-induced delayed callus bone formation in vivo. Our results provide a basis for the development of a novel strategy for the treatment of bone infection.

scholarly journals Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kornphimol Kulthong ◽  
Guido J. E. J. Hooiveld ◽  
Loes Duivenvoorde ◽  
Ignacio Miro Estruch ◽  
Victor Marin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Culture Conditions ◽  
Gene Set Enrichment Analysis ◽  
Shear Stresses ◽  
Static Culture ◽  
Dynamic Culture ◽  
Intestinal Segments ◽  
On Chip

AbstractGut-on-chip devices enable exposure of cells to a continuous flow of culture medium, inducing shear stresses and could thus better recapitulate the in vivo human intestinal environment in an in vitro epithelial model compared to static culture methods. We aimed to study if dynamic culture conditions affect the gene expression of Caco-2 cells cultured statically or dynamically in a gut-on-chip device and how these gene expression patterns compared to that of intestinal segments in vivo. For this we applied whole genome transcriptomics. Dynamic culture conditions led to a total of 5927 differentially expressed genes (3280 upregulated and 2647 downregulated genes) compared to static culture conditions. Gene set enrichment analysis revealed upregulated pathways associated with the immune system, signal transduction and cell growth and death, and downregulated pathways associated with drug metabolism, compound digestion and absorption under dynamic culture conditions. Comparison of the in vitro gene expression data with transcriptome profiles of human in vivo duodenum, jejunum, ileum and colon tissue samples showed similarities in gene expression profiles with intestinal segments. It is concluded that both the static and the dynamic gut-on-chip model are suitable to study human intestinal epithelial responses as an alternative for animal models.

scholarly journals Microdialysis for the Assessment of Intervertebral Disc and Vertebral Cancellous Bone Metabolism in a Large Porcine Model

In Vivo ◽  
2020 ◽  
Vol 34 (2) ◽  
pp. 527-532
Author(s):  
MATS BUE ◽  
PELLE HANBERG ◽  
MAJA B. THOMASSEN ◽  
MIKKEL TØTTRUP ◽  
THEIS M. THILLEMANN ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Bone Metabolism ◽  
Cancellous Bone ◽  
Porcine Model ◽  
Vertebral Cancellous Bone

Assessment of the Directional Elastic Moduli of Ewe Vertebral Cancellous Bone by Vibrational Testing

10.1114/1.153 ◽  
1999 ◽  
Vol 27 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Paul S. Weinhold ◽  
Simon C. Roe ◽  
Jerome A. Gilbert ◽  
C. Frank Abrams
Keyword(s):  
Cancellous Bone ◽  
Elastic Moduli ◽  
Vertebral Cancellous Bone

THE INFLUENCE OF TIBIAL PROSTHESIS DESIGN FEATURES ON STRESSES RELATED TO ASEPTIC LOOSENING AND STRESS SHIELDING

2011 ◽  
Vol 11 (01) ◽  
pp. 55-72 ◽  
Author(s):  
DESMOND Y. R. CHONG ◽  
ULRICH N. HANSEN ◽  
ANDREW A. AMIS
Keyword(s):  
Bone Resorption ◽  
Bone Cement ◽  
Aseptic Loosening ◽  
Cancellous Bone ◽  
Stress Shielding ◽  
Cement Mantle ◽  
Shear Stresses ◽  
Knee Prostheses ◽  
Design Features ◽  
Cement Interface

Aseptic loosening caused by mechanical factors is a recognized failure mode for tibial components of knee prostheses. This parametric study investigated the effects of prosthesis fixation design changes, which included the presence, length and diameter of a central stem, the use of fixation pegs beneath the tray, all-polyethylene versus metal-backed tray, prosthesis material stiffness, and cement mantle thickness. The cancellous bone compressive stresses and bone–cement interfacial shear stresses, plus the reduction of strain energy density in the epiphyseal cancellous bone, an indication of the likelihood of component loosening, and bone resorption secondary to stress shielding, were examined. Design features such as longer stems reduced bone and bone–cement interfacial stresses thus the risk of loosening is potentially minimized, but at the expense of an increased tendency for bone resorption. The conflicting trend suggested that bone quality and fixation stability have to be considered mutually for the optimization of prosthesis designs. By comparing the bone stresses and bone–cement shear stresses to reported fatigue strength, it was noted that fatigue of both the cancellous bone and bone–cement interface could be the driving factor for long-term aseptic loosening for metal-backed tibial trays.

Vertebral cancellous bone turn-over: Microcallus and bridges in backscatter electron microscopy

Micron ◽  
2005 ◽  
Vol 36 (7-8) ◽  
pp. 710-714 ◽  
Author(s):  
X. Banse ◽  
J.P. Devogelaer ◽  
D. Holmyard ◽  
M. Grynpas
Keyword(s):  
Electron Microscopy ◽  
Cancellous Bone ◽  
Backscatter Electron ◽  
Turn Over ◽  
Vertebral Cancellous Bone
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