Nitric Oxide Delivery System for Cell Culture Studies

2003 ◽  
Vol 31 (1) ◽  
pp. 65-79 ◽  
Author(s):  
Chen Wang ◽  
William M. Deen
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Delivery System ◽  
Culture Studies ◽  
Nitric Oxide Delivery

scholarly journals A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture

Micromachines ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 979
Author(s):  
Samineh Barmaki ◽  
Daniela Obermaier ◽  
Esko Kankuri ◽  
Jyrki Vuola ◽  
Sami Franssila ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Microfluidic Chip ◽  
Nitric Oxide Donor ◽  
Microfluidic Channels ◽  
Low Oxygen ◽  
Oxygen Scavenger ◽  
Hek 293 ◽  
Human Embryonic Kidney Cells ◽  
Nitric Oxide Delivery

A hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human embryonic kidney cells (HEK-293). The chip utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as the oxygen scavenger in one of the channels. The nitric oxide microenvironment was created by sodium nitroprusside as the light-activated nitric oxide donor in the other channel. The solutions are separated from the cell culture by a 30 µm thick gas-permeable, but liquid-impermeable polydimethylsiloxane membrane. We show that the architecture is preliminarily feasible to define the gaseous microenvironment of a cell culture in the 100 µm and 1 mm length scales.

scholarly journals Cell type-dependent release of nitric oxide and/or reactive nitrogenoxide species from intracellular SIN-1: effects on cellular NAD(P)H

2004 ◽  
Vol 385 (7) ◽  
Author(s):  
A.U. Swintek ◽  
S. Christoph ◽  
F. Petrat ◽  
H. de Groot ◽  
M. Kirsch
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Culture Studies ◽  
Aortic Endothelial Cells ◽  
No Formation ◽  
Cell Culture Systems ◽  
Zone Electrophoresis ◽  
Capillary Zone

AbstractSIN-1 is frequently used in cell culture studies as an extracellularly operating generator of peroxynitrite. However, little is known about the nature of the reactive species produced intracellulary from SIN-1. SIN-1 can easily penetrate cells as exemplified for both L-929 mouse fibroblasts and bovine aortic endothelial cells (BAECs) by utilizing capillary zone electrophoresis. In L-929 cells, SIN-1 produced nitric oxide (NO) as monitored by the fluorescent NO scavenger FNOCT-1 and by means of a NO electrode, as well as reactive nitrogenoxide species (RNOS, e.g. peroxynitrite, nitrogen dioxide, dinitrogen trioxide), as detected with the fluorescent indicator DAF-2. Laser scanning microscopy revealed that in L-929 cells SIN-1-derived species initially oxidized the major fraction of the NAD(P)H within the cytosol and the nuclei, whereas the mitochondrial NAD(P)H level was somewhat increased. In marked contrast to this, in BAECs no evidence for NO formation was found although the intracellular amount of SIN-1 was fourfold higher than in L-929 cells. In BAECs, the level of NAD(P)H was slightly decreased within the first 10 min after administration of SIN-1 in both the cytosol/nuclei and mitochondria. These observations reflect the capability of SIN-1 to generate intracellularly either almost exclusively RNOS as in BAECs, or RNOS and freely diffusing NO as in L-929 cells. Nitric oxide as well as RNOS may decisively affect cellular metabolism as indicated by the alterations in the NAD(P)H level. Hence, care should be taken when applying SIN-1 as an exclusively peroxynitrite-generating compound in cell culture systems.

S-Nitrosophytochelatins: Investigation of the Bioactivity of an Oligopeptide Nitric Oxide Delivery System

2011 ◽  
Vol 12 (6) ◽  
pp. 2103-2113 ◽  
Author(s):  
Lamia Heikal ◽  
Philip I. Aaronson ◽  
Albert Ferro ◽  
Manasi Nandi ◽  
Gary P. Martin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Delivery System ◽  
Nitric Oxide Delivery

scholarly journals Nitric oxide delivery system

1995 ◽  
Vol 74 (5) ◽  
pp. 630-631 ◽  
Author(s):  
K.P. KELLY ◽  
S.C. DEEHAN ◽  
Z.S. GRANT
Keyword(s):  
Nitric Oxide ◽  
Delivery System ◽  
Nitric Oxide Delivery

scholarly journals Ideation and assessment of a nitric oxide delivery system for spontaneously breathing subjects

Nitric Oxide ◽  
2020 ◽  
Vol 104-105 ◽  
pp. 29-35 ◽  
Author(s):  
Stefano Gianni ◽  
Caio C.A. Morais ◽  
Grant Larson ◽  
Riccardo Pinciroli ◽  
Ryan Carroll ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Delivery System ◽  
Nitric Oxide Delivery ◽  
Spontaneously Breathing
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