scholarly journals Electrotonic suppression of early afterdepolarizations in the neonatal rat ventricular myocyte monolayer

2013 ◽  
Vol 591 (21) ◽  
pp. 5357-5364 ◽  
Author(s):  
Herman D. Himel ◽  
Alan Garny ◽  
Penelope J. Noble ◽  
Raj Wadgaonkar ◽  
Joseph Savarese ◽  
...  
Keyword(s):  
Neonatal Rat ◽  
Ventricular Myocyte ◽  
Early Afterdepolarizations ◽  
Rat Ventricular Myocyte

scholarly journals Anisotropic conduction block and reentry in neonatal rat ventricular myocyte monolayers

2011 ◽  
Vol 300 (1) ◽  
pp. H271-H278 ◽  
Author(s):  
Carlos de Diego ◽  
Fuhua Chen ◽  
Yuanfang Xie ◽  
Rakesh K. Pai ◽  
Leonid Slavin ◽  
...  
Keyword(s):  
Gap Junction ◽  
Conduction Block ◽  
Neonatal Rat ◽  
Fiber Direction ◽  
Ventricular Myocyte ◽  
Na Current ◽  
Current Availability ◽  
Rapid Pacing ◽  
Rat Ventricular Myocyte ◽  
Junction Conductance

Anisotropy can lead to unidirectional conduction block that initiates reentry. We analyzed the mechanisms in patterned anisotropic neonatal rat ventricular myocyte monolayers. Voltage and intracellular Ca (Cai) were optically mapped under the following conditions: extrastimulus (S1S2) testing and/or tetrodotoxin (TTX) to suppress Na current availability; heptanol to reduce gap junction conductance; and incremental rapid pacing. In anisotropic monolayers paced at 2 Hz, conduction velocity (CV) was faster longitudinally than transversely, with an anisotropy ratio [AR = CVL/CVT, where CVL and CVT are CV in the longitudinal and transverse directions, respectively], averaging 2.1 ± 0.8. Interventions decreasing Na current availability, such as S1S2 pacing and TTX, slowed CVL and CVT proportionately, without changing the AR. Conduction block preferentially occurred longitudinal to fiber direction, commonly initiating reentry. Interventions that decreased gap junction conductance, such as heptanol, decreased CVT more than CVL, increasing the AR and causing preferential transverse conduction block and reentry. Rapid pacing resembled the latter, increasing the AR and promoting transverse conduction block and reentry, which was prevented by the Cai chelator 1,2-bis oaminophenoxy ethane- N, N, N′, N′-tetraacetic acid (BAPTA). In contrast to isotropic and uniformly anisotropic monolayers, in which reentrant rotors drifted and self-terminated, bidirectional anisotropy (i.e., an abrupt change in fiber direction exceeding 45°) caused reentry to anchor near the zone of fiber direction change in 77% of monolayers. In anisotropic monolayers, unidirectional conduction block initiating reentry can occur longitudinal or transverse to fiber direction, depending on whether the experimental intervention reduces Na current availability or decreases gap junction conductance, agreeing with theoretical predictions.

scholarly journals Simultaneous Optical Pacing and Membrane Voltage Mapping in ChR2-Expressing Neonatal Rat Ventricular Myocyte Cultures

2014 ◽  
Vol 106 (2) ◽  
pp. 383a ◽  
Author(s):  
Qince Li ◽  
Wei Kong ◽  
Ni Rong ◽  
Michael Rossi ◽  
Jing Qu ◽  
...  
Keyword(s):  
Membrane Voltage ◽  
Neonatal Rat ◽  
Ventricular Myocyte ◽  
Voltage Mapping ◽  
Rat Ventricular Myocyte

scholarly journals A Major Role for hERG in Determining Frequency of Reentry in Neonatal Rat Ventricular Myocyte Monolayer

2010 ◽  
Vol 107 (12) ◽  
pp. 1503-1511 ◽  
Author(s):  
Luqia Hou ◽  
Makarand Deo ◽  
Philip Furspan ◽  
Sandeep V. Pandit ◽  
Sergey Mironov ◽  
...  
Keyword(s):  
Neonatal Rat ◽  
Ventricular Myocyte ◽  
Rat Ventricular Myocyte

Effect of heptanol and ethanol on excitation wave propagation in a neonatal rat ventricular myocyte monolayer

2018 ◽  
Vol 51 ◽  
pp. 136-144 ◽  
Author(s):  
A.D. Podgurskaya ◽  
V.A. Tsvelaya ◽  
S.R. Frolova ◽  
I.Y. Kalita ◽  
N.N. Kudryashova ◽  
...  
Keyword(s):  
Wave Propagation ◽  
Neonatal Rat ◽  
Ventricular Myocyte ◽  
Excitation Wave ◽  
Rat Ventricular Myocyte

scholarly journals Electrophysiological Consequences of Acute Regional Ischemia/Reperfusion in Neonatal Rat Ventricular Myocyte Monolayers

Circulation ◽  
2008 ◽  
Vol 118 (23) ◽  
pp. 2330-2337 ◽  
Author(s):  
Carlos de Diego ◽  
Rakesh K. Pai ◽  
Fuhua Chen ◽  
Lai-Hua Xie ◽  
Jan De Leeuw ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Neonatal Rat ◽  
Ventricular Myocyte ◽  
Regional Ischemia ◽  
Acute Regional Ischemia ◽  
Rat Ventricular Myocyte
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