scholarly journals Action potential-triggered somatic exocytosis in mesencephalic trigeminal nucleus neurons in rat brain slices

2012 ◽  
Vol 590 (4) ◽  
pp. 753-762 ◽  
Author(s):  
Bo Zhang ◽  
Xiao-Yu Zhang ◽  
Pi-Fu Luo ◽  
Wei Huang ◽  
Fei-Peng Zhu ◽  
...  
Keyword(s):  
Action Potential ◽  
Rat Brain ◽  
Brain Slices ◽  
Trigeminal Nucleus ◽  
Mesencephalic Trigeminal Nucleus

scholarly journals Action Potential-Triggered Somatic Exocytosis in Mesencephalic Trigeminal Nucleus Neurons in Rat Brain Slices

2012 ◽  
Vol 102 (3) ◽  
pp. 319a
Author(s):  
Bo Zhang ◽  
Xiao-Yu Zhang ◽  
Wei Huang ◽  
Fei-Peng Zhu ◽  
Tao Liu ◽  
...  
Keyword(s):  
Action Potential ◽  
Rat Brain ◽  
Brain Slices ◽  
Trigeminal Nucleus ◽  
Mesencephalic Trigeminal Nucleus

scholarly journals CRYOPRESERVATION OF RAT BRAIN SLICES WITH DIFFERENT DURATION AND RESTORATION OF THEIR ELECTRICAL ACTIVITY

Biomeditsina ◽  
2019 ◽  
pp. 98-106
Author(s):  
A. A. Mokrushin
Keyword(s):  
Action Potential ◽  
Electrical Activity ◽  
Rat Brain ◽  
Brain Slices ◽  
Wistar Rat ◽  
Lateral Olfactory Tract ◽  
Olfactory Tract

Effects of cryopreservation of male Wistar rat brain slices with different duration (4, 8, 10, 23, and 90 days) on changes in their electrical activity was investigated. The amplitudes of AMPA- and NMDA-dependent glutamatergic ionotropic mechanisms were measured, as well as the action potential of the lateral olfactory tract (AP LOT) at a temperature of –20°C and subsequent warming to +37°C. After cryopreservation, these mechanisms were preserved and restored. We used the method of electrophysiological registration of the AMPA and NMDA potentials and the total AP LOT. After cryopreservation, the AMPA-dependent mechanisms and the activity of conductive LOT fi bers were restored to normothermal values. On the contrary, the recovery of NMDA-dependent mechanisms was incomplete and averaged 34% compared with the normothermal values. The results indicate that, after cryopreservation, the activity of basic ionotropic glutamatergic mechanisms in rat brain slices is restored.

Sigma receptors mediate potent neuroprotection in vivo and inhibit neuronal depolarisation and swelling in rat brain slices

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S468-S468
Author(s):  
Jennifer K Callaway ◽  
Christine Molnar ◽  
Song T Yao ◽  
Bevyn Jarrott ◽  
R David Andrew
Keyword(s):  
Rat Brain ◽  
Brain Slices ◽  
Sigma Receptors

Atypical amino acids inhibit histidine, valine, or lysine transport into rat brain

1983 ◽  
Vol 245 (4) ◽  
pp. R556-R563 ◽  
Author(s):  
J. K. Tews ◽  
A. E. Harper
Keyword(s):  
Amino Acids ◽  
Rat Brain ◽  
Blood Brain Barrier ◽  
Brain Slices ◽  
Aromatic Amino Acids ◽  
Brain Barrier ◽  
Basic Amino Acids ◽  
Large Neutral Amino Acids ◽  
Neutral Amino Acids ◽  
Single Meal

Transport of histidine, valine, or lysine into rat brain slices and across the blood-brain barrier (BBB) was determined in the presence of atypical nonprotein amino acids. Competitors of histidine and valine transport in slices were large neutral amino acids including norleucine, norvaline, alpha-aminooctanoate, beta-methylphenylalanine, and alpha-aminophenylacetate. Less effective were aromatic amino acids with ring substituents; ineffective were basic amino acids and omega-amino isomers of norleucine and aminooctanoate. Lysine transport was moderately depressed by homoarginine or ornithine plus arginine; large neutral amino acids were also similarly inhibitory. Histidine or valine transport across the BBB was also strongly inhibited by large neutral amino acids that were the most effective competitors in the slices (norvaline, norleucine, alpha-aminooctanoate, and alpha-aminophenylacetate); homoarginine and 8-aminooctanoate were ineffective. Homoarginine, ornithine, and arginine almost completely blocked lysine transport, but the large neutral amino acids were barely inhibitory. When rats were fed a single meal containing individual atypical large neutral amino acids or homoarginine, brain pools of certain large neutral amino acids or of arginine and lysine, respectively, were depleted.

scholarly journals Stimulation of Cyclic AMP Formation by Pituitary Adenylate Cyclase-Activating Polypeptide Is Attenuated by Glutamate in Rat Brain Slices

1995 ◽  
Vol 67 (4) ◽  
pp. 399-402
Author(s):  
Kaoru Kondo ◽  
Hitoshi Hashimoto ◽  
Kazuko Sakata ◽  
Hiroshi Saga ◽  
Jun-ichi Kitanaka ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Rat Brain ◽  
Brain Slices ◽  
Pituitary Adenylate Cyclase ◽  
Stimulation Of

scholarly journals Beryllium competitively inhibits brain myo-inositol monophosphatase, but unlike lithium does not enhance agonist-induced inositol phosphate accumulation

1993 ◽  
Vol 291 (2) ◽  
pp. 369-374 ◽  
Author(s):  
W S Faraci ◽  
S H Zorn ◽  
A V Bakker ◽  
E Jackson ◽  
K Pratt
Keyword(s):  
Rat Brain ◽  
Inositol Phosphate ◽  
Bipolar Depression ◽  
Neuroblastoma Cell ◽  
Brain Slices ◽  
Human Neuroblastoma Cell ◽  
Inositol Monophosphatase ◽  
Human Neuroblastoma ◽  
Phosphate Accumulation ◽  
Inositol Phosphate Accumulation

Despite limiting side-effects, lithium is the drug of choice for the treatment of bipolar depression. Its action may be due, in part, to its ability to dampen phosphatidylinositol turnover by inhibiting myo-inositol monophosphatase. Beryllium has been identified as a potent inhibitor of partially purified myo-inositol monophosphatase isolated from rat brain (Ki = 150 nM), bovine brain (Ki = 35 nM), and from the human neuroblastoma cell line SK-N-SH (Ki = 85 nM). It is over three orders of magnitude more potent than LiCl (Ki = 0.5-1.2 mM). Kinetic analysis reveals that beryllium is a competitive inhibitor of myo-inositol monophosphatase, in contrast with lithium which is an uncompetitive inhibitor. Inhibition of exogenous [3H]inositol phosphate hydrolysis by beryllium (IC50 = 250-300 nM) was observed to the same maximal extent as that seen with lithium in permeabilized SK-N-SH cells, reflecting inhibition of cellular myo-inositol monophosphatase. However, in contrast with that observed with lithium, agonist-induced accumulation of inositol phosphate was not observed with beryllium in permeabilized and non-permeabilized SK-N-SH cells and in rat brain slices. Similar results were obtained in permeabilized SK-N-SH cells when GTP-gamma-S was used as an alternative stimulator of inositol phosphate accumulation. The disparity in the actions of beryllium and lithium suggest that either (1) selective inhibition of myo-inositol monophosphatase does not completely explain the action of lithium on the phosphatidylinositol cycle, or (2) that uncompetitive inhibition of myo-inositol monophosphatase is a necessary requirement to observe functional lithium mimetic activity.

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