scholarly journals Seamless networks of myocardial bioenergetics

2011 ◽  
Vol 589 (21) ◽  
pp. 5013-5014 ◽  
Author(s):  
Jianyi Jay Zhang
Keyword(s):  
Myocardial Bioenergetics

Abstract 18663: Myocardial Heterogeneity in Heart With Postinfarction LV Remodeling and its Response to Cell Therapy

Circulation ◽  
2015 ◽  
Vol 132 (suppl_3) ◽  
Author(s):  
weina cui ◽  
lei ye ◽  
albert jang ◽  
qiang xiong ◽  
pengyuan zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Treated Group ◽  
Border Zone ◽  
Flux Rate ◽  
Lv Remodeling ◽  
Myocardial Heterogeneity ◽  
Induced Pluripotent ◽  
Myocardial Bioenergetics

Rationale and Objective: Human induced pluripotent stem cells (hiPSCs) hold promise for myocardial repair following injury. Here, we investigated the functional impact and myocardial heterogeneity of bioenergetics using a porcine model of post infarction LV remodeling, and 2 dimensional chemical shift imaging (2D CSI) P-31 MR spectroscopy. Methods and Results: Ischemia-reperfusion (I/R) injury was surgically induced by occlusion distal LAD (OCCL) for 60 minutes in female Yorkshire farm swine (≈15kg), then randomly assigned to experimental groups: 1) 16 million human induced pluripotent stem cells (hiPSC) derived cardio myocytes (CMs), smooth muscle cells (SMC) and Endothelia cells (ECs) were directly myocardial injected through an epicardial fibrin patch (P+Cell, n= 4), 2) open patch (fibrin patch with no cell) were placed over the injury site (P w/o Cell, n=4). Size matched normal (n=9) and OCCL only (n=5) pigs were also studied. Four weeks after I/R, 2D CSI MRS studies were performed in a 9.4T/ 65 cm bore magnet. In vivo myocardial energetic mapping was achieved using 31 P 2D CSI. To measure the forward flux rate PCr to ATP, 2D CSI data were acquired with or without saturation on ATPγ resonance. I/R injury has a heterogeneous effect on LV myocardial bioenergetics. Myocardial creatine phosphate (PCr)/ATP ratio is significantly decreased in border zone (BZ) of the infarction than the myocardial areas remote from the scar (RZ) in cell treated and patch only groups (1.54+/- 0.05 vs 2.25 +/- 0.10, 1.49+/-0.07 vs 2.34 +/- 0.07, BZ vs RZ, p<0.05). The BZ PCr/ATP ratio is improved in the cell treated group compared with open patch group (1.71 +/- 0.05 vs. 1.54 +/- 0.05, p<0.05). The forward flux rate constant of PCr/ATP (k pcr→ATP ) in the border zone is slightly increased in cell treated group compared with patch only group (0.29 +/- 0.02 vs 0.22 +/- 0.04 , p<0.05) Conclusion: The approach of 2D CSI 31 P MRS can effectively map the heterogeneity of myocardial ATP flux rate via CK In Vivo porcine hearts. Postinfarction LV remodeling heart manifests pronounced heterogeneity in myocardial bioenergetics with most severe alterations in BZ. Cell therapy may effectively improve BZ myocardial bioenergetics.

Abstract 5368: Igf +hgf Enhance Sca-1 + /cd31 − Cell Therapy in Hearts with Postinfarction Lv Remodeling

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Xiaohong Wang ◽  
Elizabeth Braunlin ◽  
Qingsong Hu ◽  
Joseph Lee ◽  
Qinglu Li ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Survival ◽  
Cell Transplantation ◽  
Cellular Therapy ◽  
Contractile Function ◽  
Lv Function ◽  
Beneficial Effects ◽  
Myocardial Bioenergetics

Insulin-like growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) are two potent cell survival factors in response to hypoxia and oxidative stress. We have previously shown that transplantation of Sca-1 + /CD31 − cells can improve LV function in hearts with acute myocardial infarction (AMI) although the engraftment rate was low. We hypothesized that supplementation of IGF-1 and HGF at delivery of cellular therapy can enhance the beneficial effects of Sca-1 + /CD31 − cells in the injured heart by increasing engraftment rate and endogenous cardiac cell survival. Adenovirus nuclear LacZ-labeled Sca-1 + /CD31 − cells (1x10 6 ) were injected into the ischemic area after LAD ligation in BalbC mice. Recombinant mouse IGF-1+HGF (100ng) was added to the cell suspension prior to the injection. The LV function (ECHO) and in vivo myocardial bioenergetics ( 31 P-NMR spectroscopy) were assessed 4 weeks after AMI and cell transplantation. Normal mice (Normal, n=6), and 2 control groups of LAD ligation (MI, n=6), and MI plus Sca-1 + /CD31 − cell transplantation (Cell, n=6), were compared to AMI mice with Sca-1 + /CD31 − cells transplantation plus IGF-1+HGF (Cell+IGF-1+HGF, n=6). Sca-1 + /CD31 − cells formed viable grafts and improved LV contractile function (EF: Control, 53.3+/−3.2; MI, 18.9+/−3.9; Cell, 29.1+/−5.1, p<0.05) and myocardial bioenergetics (PCr/ATP: Control, 2.13+/−0.09; MI, 1.21+/−0.09; Cell, 1.72+/−0.12; p<0.01). IGF+HGF significantly further enhanced the benefits of the cell transplantation as evidenced by significantly increased EF to 38.3+/−3.1% (p<0.05), which was accompanied by a higher cell engraftment rate (p<0.05). Using the in vitro co culture of Sca-1 + /CD31 − cells with HL-1 cells labeled by Vybrant CFDA SE cell tracer kit, we observed both IGF+HGF and Sca-1 + /CD31 − cells can inhibit TNFα and hypoxia induced HL-1 cell apoptosis (Control, 7+/−1%; TNFα, 24+/−1%; IGF+HGF+TNFα, 10+/−1%; n=6, P<0.001) and caspase 3 expression. These data demonstrate that IGF-1+HGF could serve as an adjuvant to cell transplantation for myocardial restoration.

Myocardial bioenergetics during acute hibernation

1997 ◽  
Vol 273 (3) ◽  
pp. H1452-H1463 ◽  
Author(s):  
J. Zhang ◽  
Y. Ishibashi ◽  
Y. Zhang ◽  
M. H. Eijgelshoven ◽  
D. J. Duncker ◽  
...  
Keyword(s):  
Blood Flow ◽  
Contractile Function ◽  
Creatine Phosphate ◽  
Wall Thickening ◽  
Resonance Spectroscopy ◽  
Blood Flow Ratio ◽  
Ischemic Period ◽  
Myocardial O2 Consumption ◽  
Final Extent ◽  
Myocardial Bioenergetics

During moderate reductions of blood flow, the myocardium downregulates contractile function and ATP utilization to result in reduced but stable ATP levels, recovery or stability of (reduced) creatine phosphate (CP), and preservation of myocyte viability. The intent of this study was to determine the influence of the level of ischemic blood flow and the major determinants of myocardial O2 consumption (MVO2) (heart rate and systolic blood pressure) on recovery of CP during prolonged moderate myocardial hypoperfusion. 31P-nuclear magnetic resonance spectroscopy was used to measure CP, ATP, and Pi in the subepicardium (Epi) and subendocardium (Endo) of 13 open-chest dogs. Wall thickening was measured with sonomicrometry. A coronary stenosis reduced mean myocardial blood flow (microspheres) from 1.10 +/- 0.07 to 0.71 +/- 0.06 ml.g-1.min-1 (P < 0.01) and the Endo-to-Epi blood flow ratio from 1.12 +/- 0.07 to 0.59 +/- 0.06 (P < 0.01), and dyskinesis developed. Coronary blood flow and systolic wall thickening did not change significantly during 4 h of hypoperfusion. Epi CP and ATP fell to 80 +/- 4% (P < 0.05) and 93 +/- 3% of control, respectively, at 30 min. Epi CP then recovered to 87 +/- 5% while ATP decreased further to 83 +/- 5% of baseline by the end of the 240-min ischemic period. Endo CP and ATP fell to 53 +/- 4 and 77 +/- 5% of control, respectively, at 30 min; then Endo CP recovered to 85 +/- 6% while ATP decreased further to 68 +/- 6% of baseline at 240 min of hypoperfusion. ADP levels were significantly increased at 30 min but recovered to baseline by 240 min of hypoperfusion. delta Pi/CP increased significantly (Endo > Epi) at the onset of ischemia and then progressively decreased. At 30 min, mild myocardial acidosis was observed in some hearts with variable pH recovery during continuing hypoperfusion. The data demonstrate that variations in blood flow cannot account for the magnitude of the initial fall in CP or for the final extent of recovery. However, the rate at which CP recovered was significantly correlated with the level of blood flow. Variations in the determinants of MVO2 did not account for differences in CP recovery.

scholarly journals Nicotinamide for the treatment of heart failure with preserved ejection fraction

2021 ◽  
Vol 13 (580) ◽  
pp. eabd7064
Author(s):  
Mahmoud Abdellatif ◽  
Viktoria Trummer-Herbst ◽  
Franziska Koser ◽  
Sylvère Durand ◽  
Rui Adão ◽  
...  
Keyword(s):  
Heart Failure ◽  
Ejection Fraction ◽  
Diastolic Dysfunction ◽  
Preserved Ejection Fraction ◽  
Oral Supplementation ◽  
Calcium Dependent ◽  
Cardiac Decompensation ◽  
Lower Blood ◽  
High Dietary Intake ◽  
Myocardial Bioenergetics

Heart failure with preserved ejection fraction (HFpEF) is a highly prevalent and intractable form of cardiac decompensation commonly associated with diastolic dysfunction. Here, we show that diastolic dysfunction in patients with HFpEF is associated with a cardiac deficit in nicotinamide adenine dinucleotide (NAD+). Elevating NAD+ by oral supplementation of its precursor, nicotinamide, improved diastolic dysfunction induced by aging (in 2-year-old C57BL/6J mice), hypertension (in Dahl salt-sensitive rats), or cardiometabolic syndrome (in ZSF1 obese rats). This effect was mediated partly through alleviated systemic comorbidities and enhanced myocardial bioenergetics. Simultaneously, nicotinamide directly improved cardiomyocyte passive stiffness and calcium-dependent active relaxation through increased deacetylation of titin and the sarcoplasmic reticulum calcium adenosine triphosphatase 2a, respectively. In a long-term human cohort study, high dietary intake of naturally occurring NAD+ precursors was associated with lower blood pressure and reduced risk of cardiac mortality. Collectively, these results suggest NAD+ precursors, and especially nicotinamide, as potential therapeutic agents to treat diastolic dysfunction and HFpEF in humans.

scholarly journals Lipid Resuscitation of Bupivacaine Toxicity

2011 ◽  
Vol 115 (6) ◽  
pp. 1219-1228 ◽  
Author(s):  
Zhengqian Li ◽  
Yun Xia ◽  
Xiaoxi Dong ◽  
Hongfei Chen ◽  
Fangfang Xia ◽  
...  
Keyword(s):  
Medium Chain Triglyceride ◽  
Spontaneous Circulation ◽  
Return Of Spontaneous Circulation ◽  
Lipid Resuscitation ◽  
Bupivacaine Toxicity ◽  
Myocardial Bioenergetics ◽  
Long Chain ◽  
Long Chain Triglyceride ◽  
Induced Cardiac Arrest ◽  
Observation Period

Background The superiority of Intralipid, a long-chain triglyceride (LCT) emulsion versus Lipovenoes, a long- and medium-chain triglyceride (LCT/MCT) emulsion, in reversing local anesthetic-induced cardiac arrest is poorly defined and needs to be determined. Methods The study included two parts: in experiment A, bupivacaine (20 mg/kg) was injected to produce asystole. Either Intralipid 20% (LCT group, n = 30) or Lipovenoes 20% (LCT/MCT group, n = 30) with epinephrine was infused immediately. Return of spontaneous circulation and recurrence of asystole after resuscitation were recorded. In experiment B, 80 rats using the same model and resuscitation protocol were divided into 10 groups: LCT₀, LCT₁₅, LCT₃₀, LCT₆₀, and LCT₁₂₀ and LCT/MCT₀, LCT/MCT₁₅, LCT/MCT₃₀, LCT/MCT₆₀, and LCT/MCT₁₂₀ (n = 8 each; the subscripts represent respective observation period). LCT₁₅-LCT₁₂₀ and LCT/MCT₁₅-LCT/MCT₁₂₀ groups received Intralipid 20% or Lipovenoes 20%, respectively. Plasma and myocardial bupivacaine and triglyceride concentrations, as well as myocardial bioenergetics, were determined. Results In experiment A, 24 rats in LCT group and 23 in LCT/MCT group achieved return of spontaneous circulation (P = 0.754); among them, 2 (8.3%) and 8 (34.8%) rats suffered a repeated asystole, respectively (P = 0.027). In experiment B, plasma and myocardial bupivacaine concentrations in LCT₁₅ and LCT₆₀ groups were lower than LCT/MCT₁₅ and LCT/MCT₆₀ groups, respectively. Furthermore, the plasma bupivacaine level in LCT/MCT₆₀ group was higher than LCT/MCT₃₀ group (P = 0.003). Conclusions LCT emulsion may be superior to LCT/MCT emulsion in treating bupivacaine-related cardiotoxicity as it was associated with fewer recurrences of asystole after resuscitation and lower myocardial bupivacaine concentrations.

In vivo Myocardial Bioenergetics during Acute Volume and/or Pressure Loading in a Canine Model: A 31P NMR Study

Cardiology ◽  
1989 ◽  
Vol 76 (6) ◽  
pp. 405-417 ◽  
Author(s):  
Mary Osbakken ◽  
Laszlo Ligeti ◽  
Judy Huddell ◽  
Christopher Duska ◽  
Ihor Ponomarenko ◽  
...  
Keyword(s):  
Canine Model ◽  
Pressure Loading ◽  
Nmr Study ◽  
Myocardial Bioenergetics

scholarly journals The cocoa flavanol (−)‐epicatechin exerts its cardioprotective effects by protecting myocardial bioenergetics

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Katrina Go Yamazaki ◽  
Aleksander Andreyev ◽  
Susanna Petrosyan ◽  
Guillermo Ceballos ◽  
Francisco Villarreal ◽  
...  
Keyword(s):  
Cardioprotective Effects ◽  
Myocardial Bioenergetics

scholarly journals Impaired Myocardial Bioenergetics in HFpEF and the Role of Antioxidants

2016 ◽  
Vol 10 (1) ◽  
pp. 158-162 ◽  
Author(s):  
John B. Hiebert ◽  
Qiuhua Shen ◽  
Amanda Thimmesch ◽  
Janet Pierce
Keyword(s):  
Heart Failure ◽  
Reactive Oxygen Species ◽  
Relevant Literature ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Patients With Heart Failure ◽  
Free Radical Formation ◽  
Myocardial Bioenergetics ◽  
Potential Use

Heart failure with preserved ejection fraction (HFpEF) is a significant cardiovascular condition for more than 50% of patients with heart failure. Currently, there is no effective treatment to decrease morbidity and mortality rates associated with HFpEF because of its pathophysiological heterogeneity. Recent evidence shows that deficiency in myocardial bioenergetics is one of the key pathophysiological factors contributing to diastolic dysfunction in HFpEF. Another known mechanism for HFpEF is an overproduction of free radicals, specifically reactive oxygen species. To reduce free radical formation, antioxidants are often used. This article is a summative review of the recent relevant literature that addresses cardiac bioenergetics, deficiency in myocardial bioenergetics, and increased reactive oxygen species associated with HFpEF and the promising potential use of antioxidants in managing this condition.

Glucose, insulin and potassium (GIK) during reperfusion mediates improved myocardial bioenergetics

Resuscitation ◽  
2002 ◽  
Vol 55 (3) ◽  
pp. 329-336 ◽  
Author(s):  
Mark G. Angelos ◽  
Holt N. Murray ◽  
Robert T. Gorsline ◽  
Paul F. Klawitter
Keyword(s):  
Myocardial Bioenergetics
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