scholarly journals SK2 channel expression and function in cerebellar Purkinje cells

2011 ◽  
Vol 589 (14) ◽  
pp. 3433-3440 ◽  
Author(s):  
Eric Hosy ◽  
Claire Piochon ◽  
Eva Teuling ◽  
Lorenzo Rinaldo ◽  
Christian Hansel
Keyword(s):  
Purkinje Cells ◽  
Channel Expression ◽  
Cerebellar Purkinje Cells ◽  
And Function

scholarly journals Altered Glutamate Receptor Ionotropic Delta Subunit 2 Expression in Stau2-Deficient Cerebellar Purkinje Cells in the Adult Brain

2019 ◽  
Vol 20 (7) ◽  
pp. 1797 ◽  
Author(s):  
Helena F. Pernice ◽  
Rico Schieweck ◽  
Mehrnoosh Jafari ◽  
Tobias Straub ◽  
Martin Bilban ◽  
...  
Keyword(s):  
Purkinje Cells ◽  
Glutamate Receptor ◽  
Rna Binding ◽  
Wheel Running ◽  
Adult Brain ◽  
Learning Abilities ◽  
Cerebellar Purkinje Cells ◽  
And Function ◽  
Delta Subunit

Staufen2 (Stau2) is an RNA-binding protein that is involved in dendritic spine morphogenesis and function. Several studies have recently investigated the role of Stau2 in the regulation of its neuronal target mRNAs, with particular focus on the hippocampus. Here, we provide evidence for Stau2 expression and function in cerebellar Purkinje cells. We show that Stau2 downregulation (Stau2GT) led to an increase of glutamate receptor ionotropic delta subunit 2 (GluD2) in Purkinje cells when animals performed physical activity by voluntary wheel running compared with the age-matched wildtype (WT) mice (C57Bl/6J). Furthermore, Stau2GT mice showed lower performance in motor coordination assays but enhanced motor learning abilities than did WT mice, concomitantly with an increase in dendritic GluD2 expression. Together, our results suggest the novel role of Stau2 in Purkinje cell synaptogenesis in the mouse cerebellum.

Cbln1 downregulates the formation and function of inhibitory synapses in mouse cerebellar Purkinje cells

2014 ◽  
Vol 39 (8) ◽  
pp. 1268-1280 ◽  
Author(s):  
Aya Ito-Ishida ◽  
Wataru Kakegawa ◽  
Kazuhisa Kohda ◽  
Eriko Miura ◽  
Shigeo Okabe ◽  
...  
Keyword(s):  
Purkinje Cells ◽  
Inhibitory Synapses ◽  
Cerebellar Purkinje Cells ◽  
And Function

Developmental change and function of chondroitin sulfate deposited around cerebellar Purkinje cells

2005 ◽  
Vol 82 (2) ◽  
pp. 172-183 ◽  
Author(s):  
Yumiko Shimazaki ◽  
Isao Nagata ◽  
Maki Ishii ◽  
Masahiko Tanaka ◽  
Tohru Marunouchi ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Purkinje Cells ◽  
Developmental Change ◽  
Cerebellar Purkinje Cells ◽  
And Function

scholarly journals Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells

2019 ◽  
Vol 9 (4) ◽  
pp. 80 ◽  
Author(s):  
Belozor ◽  
Yakovleva ◽  
Potapenko ◽  
Shuvaev ◽  
Smolnikova ◽  
...  
Keyword(s):  
Synaptic Transmission ◽  
Purkinje Cells ◽  
Pathological Process ◽  
Spinocerebellar Ataxia Type ◽  
Spinocerebellar Ataxia Type 1 ◽  
Cortical Organization ◽  
Neurodegenerative Process ◽  
Cerebellar Purkinje Cells ◽  
And Function

Astrogliosis is a pathological process that affects the density, morphology, and function of astrocytes. It is a common feature of brain trauma, autoimmune diseases, and neurodegeneration including spinocerebellar ataxia type 1 (SCA1), a poorly understood neurodegenerative disease. S100β is a Ca2+ binding protein. In SCA1, excessive excretion of S100β by reactive astrocytes and its uptake by Purkinje cells has been demonstrated previously. Under pathological conditions, excessive extracellular concentration of S100β stimulates the production of proinflammatory cytokines and induces apoptosis. We modeled astrogliosis by S100β injections into cerebellar cortex in mice. Injections of S100β led to significant changes in Bergmann glia (BG) cortical organization and affected their processes. S100β also changed morphology of the Purkinje cells (PCs), causing a significant reduction in the dendritic length. Moreover, the short-term synaptic plasticity and depolarization-induced suppression of synaptic transmission were disrupted after S100β injections. We speculate that these effects are the result of Ca2+-chelating properties of S100β protein. In summary, exogenous S100β induced astrogliosis in cerebellum could lead to neuronal dysfunction, which resembles a natural neurodegenerative process. We suggest that astrocytes play an essential role in SCA1 pathology, and that astrocytic S100β is an important contributor to this process.

scholarly journals Synaptic aging disrupts synaptic morphology and function in cerebellar Purkinje cells

2018 ◽  
Vol 13 (6) ◽  
pp. 1019 ◽  
Author(s):  
Lai Wang ◽  
Jin-Bo Deng ◽  
Wen-Juan Fan ◽  
Ming-Chao Yan ◽  
Yi-Zheng Sun ◽  
...  
Keyword(s):  
Purkinje Cells ◽  
Cerebellar Purkinje Cells ◽  
And Function

scholarly journals Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2277
Author(s):  
Jonas Tjaden ◽  
Annika Eickhoff ◽  
Sarah Stahlke ◽  
Julian Gehmeyr ◽  
Matthias Vorgerd ◽  
...  
Keyword(s):  
Purkinje Cells ◽  
Calcium Influx ◽  
Vegf Receptor ◽  
Channel Expression ◽  
The Central Nervous System ◽  
Cerebellar Purkinje Cells ◽  
The Impact ◽  
Ca2 Channels

T-type Ca2+ channels, generating low threshold calcium influx in neurons, play a crucial role in the function of neuronal networks and their plasticity. To further investigate their role in the complex field of research in plasticity of neurons on a molecular level, this study aimed to analyse the impact of the vascular endothelial growth factor (VEGF) on these channels. VEGF, known as a player in vasculogenesis, also shows potent influence in the central nervous system, where it elicits neuronal growth. To investigate the influence of VEGF on the three T-type Ca2+ channel isoforms, Cav3.1 (encoded by Cacna1g), Cav3.2 (encoded by Cacna1h), and Cav3.3 (encoded by Cacna1i), lasermicrodissection of in vivo-grown Purkinje cells (PCs) was performed, gene expression was analysed via qPCR and compared to in vitro-grown PCs. We investigated the VEGF receptor composition of in vivo- and in vitro-grown PCs and underlined the importance of VEGF receptor 2 for PCs. Furthermore, we performed immunostaining of T-type Ca2+ channels with in vivo- and in vitro-grown PCs and showed the distribution of T-type Ca2+ channel expression during PC development. Overall, our findings provide the first evidence that the mRNA expression of Cav3.1, Cav3.2, and Cav3.3 increases due to VEGF stimulation, which indicates an impact of VEGF on neuronal plasticity.

Ultrastorcture of Cerebellar Purkinje Cells in Rats Subjected To Partial Global Cerebral Ischemia

1985 ◽  
Vol 43 ◽  
pp. 674-675
Author(s):  
R.V.W. Dimlich ◽  
M.H. Biros
Keyword(s):  
Cerebral Ischemia ◽  
Purkinje Cells ◽  
Cell Types ◽  
Microscopic Level ◽  
Global Cerebral Ischemia ◽  
Light Microscopic Level ◽  
Cerebellar Damage ◽  
Cerebellar Injury ◽  
Cerebellar Purkinje Cells ◽  
Cell Changes

In severe cerebral ischemia, Purkinje cells of the cerebellum are one of the cell types most vulnerable to anoxic damage. In the partial (forebrain) global ischemic (PGI) model of the rat, Paljärvi noted at the light microscopic level that cerebellar damage is inconsistant and when present, milder than in the telencephalon, diencephalon and rostral brain stem. Cerebellar injury was observed in 3 of 4 PGI rats following 5 minutes of reperfusion but in none of the rats after 90 min of reperfusion. To evaluate a time between these two extremes (5 and 90 min), the present investigation used the PGI model to study the effects of ischemia on the ultrastructure of cerebellar Purkinje cells in rats that were sacrificed after 30 min of reperfusion. This time also was chosen because lactic acid that is thought to contribute to ischemic cell changes in PGI is at a maximum after 30 min of reperfusion.

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