scholarly journals Retinoic acid stimulation of VEGF secretion from human endometrial stromal cells is mediated by production of reactive oxygen species

2011 ◽  
Vol 589 (4) ◽  
pp. 863-875 ◽  
Author(s):  
Juanjuan Wu ◽  
Jason M. Hansen ◽  
Lijuan Hao ◽  
Robert N. Taylor ◽  
Neil Sidell
Keyword(s):  
Reactive Oxygen Species ◽  
Retinoic Acid ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Endometrial Stromal Cells ◽  
Stimulation Of

scholarly journals Retinoic Acid Is a Cofactor for Translational Regulation of Vascular Endothelial Growth Factor in Human Endometrial Stromal Cells

2010 ◽  
Vol 24 (1) ◽  
pp. 148-160 ◽  
Author(s):  
Neil Sidell ◽  
Yue Feng ◽  
Lijuan Hao ◽  
Juanjuan Wu ◽  
Jie Yu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Vascular Endothelial Growth Factor ◽  
Retinoic Acid ◽  
Growth Factor ◽  
Stromal Cells ◽  
Vascular Endothelial ◽  
Oxygen Species ◽  
Transcriptional Activators ◽  
Endometrial Stromal Cells ◽  
Endothelial Growth Factor

Abstract Vascular endothelial growth factor (VEGF) and endometrial angiogenesis play a critical role in successful embryonic implantation. Despite many studies of the effects of estrogen and progesterone on VEGF expression, its focal regulation at the site of implantation is unknown. Retinoic acid (RA) has been reported to regulate VEGF in a variety of cell types. Because localized RA synthesis occurs within the periimplantation endometrium, we tested the possibility that RA regulates VEGF production in endometrial stromal cells. Using primary and telomerase-immortalized human endometrial stromal cells, we determined that RA alone did not alter constitutive levels of VEGF production, but markedly amplified secretion when the cells were cotreated with activators of VEGF gene transcription (12-O-tetradecanoyl phorbol-13-acetate, TPA; TGF-β; and IL-1β). Whereas TPA or TGF-β alone stimulated VEGF promoter activity and up-regulated mRNA levels, significant protein secretion was detected only after RA was added to the culture systems. Analysis of retinoids in secretory phase endometrial biopsies indicated that endogenous RA accumulated at concentrations sufficient to induce VEGF secretion. Polyribosome profile analysis showed that the addition of RA to transcriptional activators of VEGF shifted the translational suppressed VEGF mRNA transcripts into larger polyribosome complexes engaged in active translation. Although the precise mechanism(s) of the RA effect remains to be defined, it appears to be mediated by reactive oxygen species; the antioxidant N-acetylcysteine inhibited RA+TPA-stimulated secretion of VEGF by more than 80%. Together, our results demonstrate that in human endometrial stromal cells, RA can combine with transcriptional activators of VEGF to augment VEGF secretion through a translational mechanism of action mediated by reactive oxygen species. These findings suggest a link between the spatiotemporal changes of retinoid synthesis in the periimplantation stroma and the capacity to quickly up-regulate focal VEGF secretion needed to induce early angiogenic events of pregnancy.

scholarly journals NADPH Oxidase-Derived Reactive Oxygen Species Mediate Decidualization of Human Endometrial Stromal Cells in Response to Cyclic AMP Signaling

Endocrinology ◽  
2010 ◽  
Vol 152 (2) ◽  
pp. 730-740 ◽  
Author(s):  
Marwa Al-Sabbagh ◽  
Luca Fusi ◽  
Jenny Higham ◽  
Yun Lee ◽  
Kaiyu Lei ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Stromal Cells ◽  
Rho Gtpase ◽  
Primary Cultures ◽  
Reactive Oxygen ◽  
Marker Genes ◽  
Oxygen Species ◽  
Endometrial Stromal Cells ◽  
Decidual Cells

Abstract Differentiation of human endometrial stromal cells into specialized decidual cells is critical for embryo implantation and survival of the conceptus. Initiation of this differentiation process is strictly dependent on elevated cAMP levels, but the signal intermediates that control the expression of decidual marker genes, such as prolactin (PRL) and IGFBP1, remain poorly characterized. Here we show that cAMP-dependent decidualization can be attenuated or enhanced upon treatment of primary cultures with a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor (diphenylen iodonium) or activator (apocynin), respectively. Time-course analysis demonstrated that cAMP enhances endogenous reactive oxygen species production, apparent after 12 h of stimulation, which coincides with a dramatic increase in decidual PRL and IGFBP1 expression. Knockdown of the Rho GTPase RAC1, which disables activation of the NADPH oxidase homologs NADPH oxidase (NOX)-1, NOX-2, and NOX-3, had no effect on PRL or IGFBP1 expression. In contrast, silencing of NOX-4, or its cofactor p22PHOX, inhibited the expression of both decidual markers. Finally, we show that the NOX-4/p22PHOX complex regulates the DNA-binding activity of CCAAT/enhancer binding protein-β, a key regulator of human endometrial stromal cell differentiation. Thus, NOX-4 activation and reactive oxygen species signaling play an integral role in initiating the endometrial decidual response in preparation of pregnancy.

scholarly journals Cigarette Smoke Extract Activates Hypoxia-Inducible Factors in a Reactive Oxygen Species-Dependent Manner in Stroma Cells from Human Endometrium

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 48
Author(s):  
Naoko Kida ◽  
Yoshiyuki Matsuo ◽  
Yoshiko Hashimoto ◽  
Kenichiro Nishi ◽  
Tomoko Tsuzuki-Nakao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Stromal Cells ◽  
Hypoxia Inducible Factor ◽  
Reactive Oxygen ◽  
Protein Stabilization ◽  
Human Endometrium ◽  
Dependent Manner ◽  
Oxygen Species ◽  
Endometrial Stromal Cells ◽  
Hypoxic Conditions

Cigarette smoking (CS) is a major contributing factor in the development of a large number of fatal and debilitating disorders, including degenerative diseases and cancers. Smoking and passive smoking also affect the establishment and maintenance of pregnancy. However, to the best of our knowledge, the effects of smoking on the human endometrium remain poorly understood. In this study, we investigated the regulatory mechanism underlying CS-induced hypoxia-inducible factor (HIF)-1α activation using primary human endometrial stromal cells and an immortalized cell line (KC02-44D). We found that the CS extract (CSE) increased reactive oxygen species levels and stimulated HIF-1α protein stabilization in endometrial stromal cells, and that CS-induced HIF-1α-dependent gene expression under non-hypoxic conditions in a concentration- and time-dependent manner. Additionally, we revealed the upregulated expression of a hypoxia-induced gene set following the CSE treatment, even under normoxic conditions. These results indicated that HIF-1α might play an important role in CS-exposure-induced cellular stress, inflammation, and endometrial remodeling.

scholarly journals Reactive oxygen species stimulate prostaglandin F2α production in human endometrial stromal cells in vitro

2001 ◽  
Vol 16 (9) ◽  
pp. 1797-1801 ◽  
Author(s):  
Norihiro Sugino ◽  
Ayako Karube-Harada ◽  
Shiro Kashida ◽  
Shuji Takiguchi ◽  
Hiroshi Kato
Keyword(s):  
Reactive Oxygen Species ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Prostaglandin F2α ◽  
Oxygen Species ◽  
Endometrial Stromal Cells

Adipose stromal cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity and reactive oxygen species

2013 ◽  
Vol 544 ◽  
pp. 15-19 ◽  
Author(s):  
Huiying Gu ◽  
Jimmy Wang ◽  
Nicole Du ◽  
Jiangning Tan ◽  
Brian Johnstone ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Conditioned Medium ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Adipose Stromal Cells ◽  
6 Hydroxydopamine

The impact of mitochondria and reactive oxygen species on the proliferation of human amniotic mesenchymal stromal cells

2018 ◽  
Vol 1859 ◽  
pp. e45
Author(s):  
Sergiu Dumitrescu ◽  
Adelheid Weidinger ◽  
Asmita Banerjee ◽  
Susanne Wolbank ◽  
Karlheinz Hilber ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
The Impact
Sign in / Sign up

Export Citation Format

Share Document