scholarly journals Signalling pathway of nitric oxide in synaptic GABA release in the rat paraventricular nucleus

2003 ◽  
Vol 554 (1) ◽  
pp. 100-110 ◽  
Author(s):  
De-Pei Li ◽  
Shao-Rui Chen ◽  
Thomas F. Finnegan ◽  
Hui-Lin Pan
Keyword(s):  
Nitric Oxide ◽  
Paraventricular Nucleus ◽  
Gaba Release ◽  
Signalling Pathway

scholarly journals Intermedin in Paraventricular Nucleus Attenuates Sympathetic Activity and Blood Pressure via Nitric Oxide in Hypertensive Rats

Hypertension ◽  
2014 ◽  
Vol 63 (2) ◽  
pp. 330-337 ◽  
Author(s):  
Ye-Bo Zhou ◽  
Hai-Jian Sun ◽  
Dan Chen ◽  
Tong-Yan Liu ◽  
Ying Han ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Paraventricular Nucleus ◽  
Sympathetic Activity ◽  
Hypertensive Rats

The nitric oxide-cyclic GMP signalling pathway in rat brain

1993 ◽  
Vol 32 (11) ◽  
pp. 1267-1277 ◽  
Author(s):  
Eric Southam ◽  
John Garthwaite
Keyword(s):  
Nitric Oxide ◽  
Rat Brain ◽  
Cyclic Gmp ◽  
Signalling Pathway

Abstract 085: CHIP: E3 Ubiquitin Ligase Mediates Proteasomal Degradation of Neuronal Nitric Oxide Synthase in the Paraventricular Nucleus of Rats with Heart Failure

Hypertension ◽  
2017 ◽  
Vol 70 (suppl_1) ◽  
Author(s):  
Neeru M Sharma ◽  
Kenichi Katsurada ◽  
Xuefei Liu ◽  
Kaushik P Patel
Keyword(s):  
Nitric Oxide ◽  
Heart Failure ◽  
Nitric Oxide Synthase ◽  
Paraventricular Nucleus ◽  
Ubiquitin Ligase ◽  
Protein A ◽  
Neuronal Nitric Oxide Synthase ◽  
Proteasomal Degradation ◽  
Oxide Synthase

The exaggerated sympathetic drive is a characteristic of heart failure (HF) due to reduced neuronal nitric oxide synthase (nNOS) within the paraventricular nucleus (PVN). Previously we have shown that there were increased accumulation of nNOS-ubiquitin (nNOS-Ub) conjugates in the PVN of rats with HF (1.0±0.05 Sham vs. 1.29±0.06 HF) due to the increased levels of PIN (a protein inhibitor of nNOS, known to dissociate nNOS dimers into monomers) (0.76±0.10 Sham vs. 1.12±0.09 HF) and decreased levels of tetrahydrobiopterin (BH4): a cofactor required for stabilization of nNOS dimers (0.62±0.02 Sham vs. 0.44±0.03 HF). We also showed that there is blunted nitric oxide-mediated inhibition of sympathetic tone via the PVN in HF. Here we examined whether CHIP(C-terminus of Hsp70 -interacting protein), a chaperone-dependent E3 ubiquitin-protein isopeptide ligase known to ubiquitylate Hsp90-chaperoned proteins could act as an ubiquitin ligase for nNOS in the PVN. Immunofluorescence studies revealed colocalization of nNOS and CHIP in the PVN indicating their possible interaction. CHIP expression was increased by 50% in the PVN of rats with HF(0.96±0.08 Sham vs.1.44±0.10* HF). It is shown that Hsp90 protects nNOS from ubiquitination while Hsp70 promotes the ubiquitination and degradation. We observed significant upregulation of Hsp70 (0.49±0.03 Sham vs. 0.65±0.02* HF) with a trend toward the decrease in Hsp90 expression (0.90±0.07 Sham vs. 0.71±0.06 HF). The opposing effects of the two chaperones could account for the increased CHIP-mediated ubiquitination and degradation of dysfunctional nNOS monomers in the PVN of rats with HF. Furthermore, neuronal NG108-15 cell line transfected with the pCMV3-CHIP-GFP spark (CHIP overexpression plasmid) showed approximately 74% increase in CHIP with concomitant 49% decrease in nNOS expression. In vitro ubiquitination assay in NG108 cells transfected with pCMV-(HA-Ub) 8 and pCMV3-CHIP-GFP spark plasmid reveal increased HA-Ub-nNOS conjugates (1.13 ± 0.09 Scramble vs. 1.65 ± 0.12* CHIP plasmid). Taken together, our results identify CHIP as an E3 ligase for ubiquitination of dysfunctional nNOS and CHIP expression is augmented during HF leading to increased proteasomal degradation of nNOS in the PVN.

The Nitric Oxide Signalling Pathway in Aortic Aneurysm and Dissection

2021 ◽  
Author(s):  
Marta Toral ◽  
Andrea Fuente‐Alonso ◽  
Miguel R. Campanero ◽  
Juan Miguel Redondo
Keyword(s):  
Nitric Oxide ◽  
Aortic Aneurysm ◽  
Signalling Pathway ◽  
Aortic Aneurysm And Dissection

scholarly journals Functional significance of nitric oxide in ionomycin-evoked [3H]GABA release from mouse cerebral cortical neurons

2002 ◽  
Vol 81 (1) ◽  
pp. 130-141 ◽  
Author(s):  
Masashi Katsura ◽  
Keijiro Shuto ◽  
Yutaka Mohri ◽  
Makoto Shigeto ◽  
Seitaro Ohkuma
Keyword(s):  
Nitric Oxide ◽  
Cortical Neurons ◽  
Functional Significance ◽  
Gaba Release ◽  
Cerebral Cortical Neurons

scholarly journals Regulation of arterial pressure by the paraventricular nucleus in conscious rats: interactions among glutamate, GABA, and nitric oxide

2013 ◽  
Vol 3 ◽  
Author(s):  
Marli C. Martins-Pinge ◽  
Patrick J. Mueller ◽  
C. Michael Foley ◽  
Cheryl M. Heesch ◽  
Eileen M. Hasser
Keyword(s):  
Nitric Oxide ◽  
Arterial Pressure ◽  
Paraventricular Nucleus ◽  
Conscious Rats

scholarly journals Endocannabinoid and nitric oxide systems of the hypothalamic paraventricular nucleus mediate effects of NPY on energy expenditure

2018 ◽  
Vol 18 ◽  
pp. 120-133 ◽  
Author(s):  
Zoltán Péterfi ◽  
Imre Farkas ◽  
Raphael G.P. Denis ◽  
Erzsébet Farkas ◽  
Motokazu Uchigashima ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Energy Expenditure ◽  
Paraventricular Nucleus ◽  
Hypothalamic Paraventricular Nucleus ◽  
Oxide Systems
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