scholarly journals Altered inhibitory synaptic transmission in superficial dorsal horn neurones in spastic and oscillator mice

2003 ◽  
Vol 551 (3) ◽  
pp. 905-916 ◽  
Author(s):  
B A Graham ◽  
P R Schofield ◽  
P Sah ◽  
R J Callister
Keyword(s):  
Synaptic Transmission ◽  
Dorsal Horn ◽  
Superficial Dorsal Horn ◽  
Inhibitory Synaptic Transmission ◽  
Dorsal Horn Neurones

Facilitatory Actions of Serotonin Type 3 Receptors on GABAergic Inhibitory Synaptic Transmission in the Spinal Superficial Dorsal Horn

2009 ◽  
Vol 102 (3) ◽  
pp. 1459-1471 ◽  
Author(s):  
Teruyuki Fukushima ◽  
Toshiki Ohtsubo ◽  
Masayuki Tsuda ◽  
Yuchio Yanagawa ◽  
Yuuichi Hori
Keyword(s):  
Synaptic Transmission ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Gabaergic Neurons ◽  
Pcr Analysis ◽  
Superficial Dorsal Horn ◽  
Inhibitory Synaptic Transmission ◽  
Spinal Dorsal ◽  
Presynaptic Mechanisms ◽  
Type 3

Analgesic effects of serotonin (5-hydroxytryptamine [5-HT]) type 3 (5-HT3) receptors may involve the release of γ-aminobutyric acid (GABA) in the spinal dorsal horn. However, the precise synaptic mechanisms for 5-HT3 receptor-mediated spinal analgesia are not clear. In this study, we investigated whether GABAergic neurons in the superficial dorsal horn (SDH) express functional 5-HT3 receptors and how these 5-HT3 receptors affect GABAergic inhibitory synaptic transmission in the SDH, by using slice preparations from adult glutamate decarboxylase 67–green fluorescent protein (GAD67-GFP) knock-in mice. Tight-seal whole cell recordings from GFP-positive and -negative neurons showed that 5-HT3 receptor-specific agonist 2-methyl-serotonin (2-Me-5-HT) induced inward currents in a substantial population of both GFP-positive and -negative neurons. Additionally, we confirmed expression of 5-HT3 receptors in both types of neurons by single-cell reverse transcription–polymerase chain reaction (RT-PCR) analysis. Further, GABAA receptor-mediated inhibitory postsynaptic currents (IPSCs)—both those evoked by electrical stimulation and those occurring spontaneously in tetrodotoxin (i.e., miniature IPSCs [mIPSCs])—were recorded from GFP-negative neurons. 2-Me-5-HT increased the amplitude of the evoked IPSCs and the frequency of mIPSCs. The amplitude of mIPSCs was not affected by 2-Me-5-HT, suggesting that 5-HT augments GABAergic synaptic transmission via presynaptic mechanisms. The present observations indicate that 5-HT3 receptors are expressed on both somadendritic regions and presynaptic terminals of GABAergic neurons and regulate GABAA receptor-mediated inhibitory synaptic transmission in the SDH. Taken together, these results provide clues for the underlying mechanisms of the antinociceptive actions of 5-HT3 receptors in the spinal dorsal horn.

scholarly journals Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones

2004 ◽  
Vol 559 (3) ◽  
pp. 707-713 ◽  
Author(s):  
Maiko Moriyama ◽  
Hidemasa Furue ◽  
Toshihiko Katafuchi ◽  
Hitoshi Teranishi ◽  
Takahiro Sato ◽  
...  
Keyword(s):  
Synaptic Transmission ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Presynaptic Modulation ◽  
Spinal Dorsal ◽  
Dorsal Horn Neurones
Sign in / Sign up

Export Citation Format

Share Document