scholarly journals Na+ channel inactivation: a comparative study between pancreatic islet  -cells and adrenal chromaffin cells in rat

2003 ◽  
Vol 548 (1) ◽  
pp. 191-202 ◽  
Author(s):  
X.-L. Lou ◽  
X. Yu ◽  
X.-K. Chen ◽  
K.-L. Duan ◽  
L.-M. He ◽  
...  
Keyword(s):  
Comparative Study ◽  
Pancreatic Islet ◽  
Chromaffin Cells ◽  
Islet Cells ◽  
Na Channel ◽  
Pancreatic Islet Cells ◽  
Adrenal Chromaffin Cells ◽  
Channel Inactivation ◽  
Adrenal Chromaffin

scholarly journals Down-regulation of Na channel α-subunit mRNA by protein kinase C e in adrenal chromaffin cells

1997 ◽  
Vol 73 ◽  
pp. 157
Author(s):  
Toshihiko Yanagita ◽  
Hideyuki Kobayashi ◽  
Keizou Masumoto ◽  
Ryuichi Yamamoto ◽  
Tomoaki Yuhi ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Chromaffin Cells ◽  
Na Channel ◽  
Adrenal Chromaffin Cells ◽  
Down Regulation ◽  
Α Subunit ◽  
Subunit Mrna ◽  
Kinase C ◽  
Adrenal Chromaffin

scholarly journals Neurons and neuroendocrine cells contain chromogranin: detection of the molecule in normal bovine tissues by immunochemical and immunohistochemical methods.

1985 ◽  
Vol 33 (8) ◽  
pp. 791-798 ◽  
Author(s):  
J A Nolan ◽  
J Q Trojanowski ◽  
R Hogue-Angeletti
Keyword(s):  
Molecular Weight ◽  
Submandibular Gland ◽  
Chromaffin Cells ◽  
Islet Cells ◽  
Neuroendocrine Cells ◽  
Thymic Epithelial Cells ◽  
Adrenal Chromaffin Cells ◽  
Thyroid C Cells ◽  
Adrenal Chromaffin ◽  
Immunohistochemical Studies

Gel-eluted bovine chromogranin (CG), the 75,000 dalton acidic protein abundantly present in adrenal chromaffin granules, was used as immunogen to prepare anti-CG serum. The specificity of the antiserum was demonstrated in immunoblots of electrophoresed bovine CG and in immunohistochemical studies of bovine adrenal medulla. In the immunoblots, the predominant immunoreactive band had a molecular weight of 75,000 daltons. Bands with a higher or lower molecular weight were also immunoreactive and may represent CG precursors or breakdown products. In the adrenal gland, only adrenal chromaffin cells contained CG immunoreactivity. Immunoblots and immunohistochemistry were also used to characterize the distribution of CG in bovine tissues. CG was expressed by cells of the diffuse neuroendocrine system (DNS) including: adrenal chromaffin cells, enterochromaffin cells, pancreatic islet cells, cells of the adenohypophysis, thyroid C cells, parathyroid cells, and submandibular gland. CG was also seen in four locations not previously recognized to express this antigen: thymic epithelial cells, neurons, the inner segment of rods and cones, and the submandibular gland. We demonstrate a wider distribution of CG than previously recognized and that the molecule detected in tissue by immunohistochemistry is indeed CG. We conclude that CG is expressed by neurons, cells of the DNS, and by a few other cells that may or may not be related to the DNS. The antiserum described here should prove valuable in developing an understanding of the function(s) of CG.

Morphological Observations on the Granule Types in Rabbit Extra-Adrenal Chromaffin Cells.

1975 ◽  
Vol 33 ◽  
pp. 318-319
Author(s):  
Joe A. Mascorro ◽  
Robert D. Yates
Keyword(s):  
Chromaffin Cells ◽  
Sodium Phosphate ◽  
Ganglion Cells ◽  
Ultrastructural Level ◽  
Osmic Acid ◽  
Adrenal Chromaffin Cells ◽  
Potassium Iodate ◽  
Perfusion Fluid ◽  
New Zealand White Rabbits ◽  
Adrenal Chromaffin

Extra-adrenal chromaffin organs (abdominal paraganglia) constitute rich sources of catecholamines. It is believed that these bodies contain norepinephrine exclusively. However, the present workers recently observed epinephrine type granules in para- ganglion cells. This report investigates catecholamine containing granules in rabbit paraganglia at the ultrastructural level.New Zealand white rabbits (150-170 grams) were anesthetized with 50 mg/kg Nembutal (IP) and perfused with 3% glutaraldehyde buffered with 0.2M sodium phosphate, pH 7.3. The retroperitoneal tissue blocks were removed and placed in perfusion fluid for 4 hours. The abdominal paraganglia were dissected from the blocks, diced, washed in phosphate buffer and fixed in 1% osmic acid buffered with phosphate. In other animals, the glutaraldehyde perfused tissue blocks were immersed for 1 hour in 3% glutaraldehyde/2.5% potassium iodate buffered as before. The paraganglia were then diced, separated into two vials and washed in the buffer. A portion of this tissue received osmic acid fixation.

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts

Diabetes ◽  
1980 ◽  
Vol 29 (6) ◽  
pp. 497-500 ◽  
Author(s):  
P. Meda ◽  
E. L. Hooghe-Peters ◽  
L. Orci
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Monolayer Cultures

Use of liposomes to introduce substances into pancreatic islet cells

Diabetes ◽  
1988 ◽  
Vol 37 (8) ◽  
pp. 1123-1128
Author(s):  
N. Welsh ◽  
A. Hallberg ◽  
S. Sandler ◽  
C. Hellerstrom
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Superfusion of dissociated pancreatic islet cells attached to Cytodex beads

Diabetes ◽  
1982 ◽  
Vol 31 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Y. Spiess ◽  
M. A. Smith ◽  
W. Vale
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Efficient gene transfer to dispersed human pancreatic islet cells in vitro using adenovirus-polylysine/DNA complexes or polycationic liposomes

Diabetes ◽  
1996 ◽  
Vol 45 (9) ◽  
pp. 1197-1203 ◽  
Author(s):  
J. Saldeen ◽  
D. T. Curiel ◽  
D. L. Eizirik ◽  
A. Andersson ◽  
E. Strandell ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Dna Complexes ◽  
Human Pancreatic Islet ◽  
Efficient Gene
Sign in / Sign up

Export Citation Format

Share Document