scholarly journals Multiple conductance states of single Ca2+-activated Cl- channels in rabbit pulmonary artery smooth muscle cells

2003 ◽  
Vol 547 (1) ◽  
pp. 181-196 ◽  
Author(s):  
A S Piper ◽  
W A Large
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Rabbit Pulmonary Artery ◽  
Cl Channels ◽  
Pulmonary Artery Smooth Muscle ◽  
Conductance States ◽  
Multiple Conductance States

Endothelin-1 increases intracellular Ca2+ in rabbit pulmonary artery smooth muscle cells through phospholipase C

2005 ◽  
Vol 289 (4) ◽  
pp. H1551-H1559 ◽  
Author(s):  
Eun A. Ko ◽  
Won Sun Park ◽  
Jae-Hong Ko ◽  
Jin Han ◽  
Nari Kim ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Phospholipase C ◽  
Muscle Cells ◽  
Transient Increase ◽  
Rabbit Pulmonary Artery ◽  
Intracellular Ca ◽  
Pulmonary Artery Smooth Muscle ◽  
Sustained Increase

In freshly isolated rabbit pulmonary artery smooth muscle cells, endothelin (ET)-1 induced a transient increase in intracellular Ca2+ concentration ([Ca2+]i) followed by a return to the initial [Ca2+]i. This response was not abolished by the voltage-dependent Ca2+ channel blocker nicardipine or removal of Ca2+ from the bath solution but was inhibited by ryanodine and thapsigargin. This finding suggested that the increase in [Ca2+]i induced by ET-1 was attributable to release of Ca2+ from ryanodine- and inositol 1,4,5-trisphosphate-sensitive intracellular Ca2+ stores. The transient increase in [Ca2+]i induced by ET-1 was also inhibited by pretreatment with antagonists of ET type A and B (ETA and ETB) receptors (BQ-123 and BQ-788, respectively). Furthermore, the ETB receptor agonist IRL-1620 induced an increase in [Ca2+]i that was followed by a sustained increase in [Ca2+]i; the sustained increase in [Ca2+]i was blocked by nicardipine. Using the nystatin-perforated patch-clamp technique, we found that IRL-1620 caused an increase in Ca2+ current that was inhibited by addition of ET-1. ET-1 did not inhibit Ca2+ current when cells were pretreated with BQ-123. These results suggested that when both receptor types are activated, the opposing responses lead to abolition of the sustained [Ca2+]i increases induced by ETB receptor activation. Western blot analysis confirmed expression of ETA and ETB receptors. Finally, U-73122 inhibited the ET-1-induced [Ca2+]i increase, indicating that phospholipase C was involved in modulation of the ET-1-induced [Ca2+]i increase in rabbit pulmonary artery smooth muscle cells.

scholarly journals Calcineurin Aα but Not Aβ Augments ICl(Ca)in Rabbit Pulmonary Artery Smooth Muscle Cells

2004 ◽  
Vol 279 (37) ◽  
pp. 38830-38837 ◽  
Author(s):  
Iain A. Greenwood ◽  
Jonathan Ledoux ◽  
Amy Sanguinetti ◽  
Brian A. Perrino ◽  
Normand Leblanc
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Rabbit Pulmonary Artery ◽  
Pulmonary Artery Smooth Muscle

scholarly journals Potential Role of PP2A in Regulating Ca 2+ ‐Activated Cl − Channels in Pulmonary Artery Smooth Muscle Cells

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Ramon Ayon ◽  
William Sones ◽  
Abigail Forrest ◽  
Brian Perrino ◽  
Maria Valencik ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Potential Role ◽  
Muscle Cells ◽  
Cl Channels ◽  
Pulmonary Artery Smooth Muscle
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