scholarly journals Oscillations in ciliary beat frequency and intracellular calcium concentration in rabbit tracheal epithelial cells induced by ATP

2003 ◽  
Vol 546 (3) ◽  
pp. 733-749 ◽  
Author(s):  
Luo Zhang ◽  
Michael J. Sanderson
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Intracellular Calcium Concentration ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial ◽  
Ciliary Beat

Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells

2002 ◽  
Vol 92 (6) ◽  
pp. 2396-2402 ◽  
Author(s):  
Jeffrey I. Frohock ◽  
Corrine Wijkstrom-Frei ◽  
Matthias Salathe
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Single Cells ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Airway Epithelial Cells ◽  
Racemic Mixture ◽  
Dependent Manner ◽  
Tracheal Epithelial Cells ◽  
Ciliary Beat

β2-Adrenergic agonists stimulate ciliary beat frequency (CBF), an integral part of mucociliary clearance. To evaluate the differential effects of albuterol enantiomers and their racemic mixture on ciliary function, CBF and intracellular calcium were measured at room temperature from single ovine airway epithelial cells with use of digital videomicroscopy. Baseline CBF was 7.2 ± 0.2 (SE) Hz ( n = 80 measurements). R-albuterol (10 μM to 1 mM) stimulated CBF in a dose-dependent manner to maximally 24.4 ± 5.4% above baseline. Racemic albuterol stimulated CBF to maximally 12.8 ± 3.6% above baseline, a significantly lower increase compared with R-albuterol alone, despite identical R-enantiomer amounts in both groups. Simultaneous recordings of intracellular calcium concentration and CBF from single cells indicated that the CBF increase in response to R-albuterol was mediated through β-receptors and stimulation of protein kinase A, in a calcium-dependent and -independent fashion. S-albuterol had a negligible effect on CBF and did not change intracellular calcium. Together, these results suggest that R-albuterol is more efficacious than racemic albuterol in stimulating CBF. Thus S-albuterol may interfere with the ability of R-albuterol to increase CBF.

Muscarinic signaling in ciliated tracheal epithelial cells: dual effects on Ca2+ and ciliary beating

1997 ◽  
Vol 272 (2) ◽  
pp. L301-L310 ◽  
Author(s):  
M. Salathe ◽  
E. J. Lipson ◽  
P. I. Ivonnet ◽  
R. J. Bookman
Keyword(s):  
Epithelial Cells ◽  
Muscarinic Receptors ◽  
Single Cells ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Tracheal Epithelial Cells ◽  
M3 Receptors ◽  
Tracheal Epithelial ◽  
M3 Subtype ◽  
Microscopy Techniques

To examine cholinergic signal transduction pathways that modulate ciliary beat frequency (CBF), cultured ovine tracheal epithelial cells were imaged using a combination of phase-contrast (CBF) and fluorescence (Ca2+) microscopy techniques. In single cells, acetylcholine (ACh) transiently increased CBF and intracellular Ca2+ concentration ([Ca2+]i), mainly by Ca2+ release from internal stores, with a small delayed contribution from Ca2+ influx. Nicotinic agonists did not alter CBF or [Ca2+]i, whereas atropine blocked the ACh-stimulated transients, consistent with the involvement of muscarinic receptors. 4-Diphenylacetoxy-N-methylpiperidine methiodide was approximately 100 times more potent than pirenzepine in inhibiting the ACh-induced [Ca2+]i peaks, suggesting that the receptor is a pharmacologically defined (M3) subtype. Interestingly, after depletion of intracellular Ca2+ stores by thapsigargin, ACh caused a rapid transient decrease in both CBF and [Ca2+]i, again with an antagonist profile of M3 receptors. We conclude that activation of M3 muscarinic receptors initiates specific signaling pathways that act simultaneously to increase and decrease [Ca2+]i and CBF.

cAMP does not regulate [Ca2+]i in human tracheal epithelial cells in primary culture

1994 ◽  
Vol 107 (10) ◽  
pp. 2899-2907
Author(s):  
P.B. Davis ◽  
C.L. Silski ◽  
A. Perez
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Primary Culture ◽  
Time Course ◽  
Calcium Concentration ◽  
Second Messengers ◽  
Tracheal Epithelial Cells ◽  
Adrenergic Response ◽  
Tracheal Epithelial

Human tracheal epithelial cells in primary culture respond to different receptor agonists with different peak intracellular calcium concentrations. From resting concentration 138 +/- 13 nM, bradykinin (0.1 microM) produces an increase to a maximum of 835 +/- 195 nM, histamine (10 microM) to 352 +/- 51 nM, and ATP (5-500 microM) to more than 1500 nM. Nine of 14 cultures also responded to isoproterenol (10 microM), though with a smaller increase, to 210 +/- 29 nM. A response was observed with isoproterenol, and epinephrine, but not norepinephrine, phenylephrine or methoxamine, was inhibited by propranolol but not phentolamine, and so this appeared to be a beta-adrenergic response. However, no response could be detected to adenosine, prostaglandin E2 or forskolin, agents that activate adenylate cyclase, or to permeant analogs of cAMP (CPT-cAMP or db-cAMP). The intracellular calcium response to isoproterenol did not follow either the time-course or the desensitization pattern of the cAMP response. Thus, this response to isoproterenol is not mediated by cAMP. No relation was demonstrated between cAMP production by other agonists and the response of intracellular calcium. Pretreatment with agents that increase cAMP did not affect the calcium responses to ATP or bradykinin. Thus, cAMP does not regulate intracellular calcium concentration in human tracheal epithelial cells. The variation in peak intracellular calcium responses to various agonists may be explained by the presence of multiple second messengers (other than cAMP), multiple intracellular pools of calcium, or cell heterogeneity. The agonists tested had the same relative potency in cells from patients with cystic fibrosis as in non-cystic fibrosis cells.

Betadine has a ciliotoxic effect on ciliated human respiratory cells

2014 ◽  
Vol 129 (S1) ◽  
pp. S45-S50 ◽  
Author(s):  
J H Kim ◽  
J Rimmer ◽  
N Mrad ◽  
S Ahmadzada ◽  
R J Harvey
Keyword(s):  
Epithelial Cells ◽  
High Speed ◽  
Imaging System ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Positive Control ◽  
Clinical Dose ◽  
Significant Difference ◽  
Sinonasal Mucosa ◽  
Ciliary Beat

AbstractObjective:This study investigated the effect of Betadine on ciliated human respiratory epithelial cells.Methods:Epithelial cells from human sinonasal mucosa were cultured at the air–liquid interface. The cultures were tested with Hanks' balanced salt solution containing 10 mM HEPES (control), 100 µM ATP (positive control), 5 per cent Betadine or 10 per cent Betadine (clinical dose). Ciliary beat frequency was analysed using a high-speed camera on a computer imaging system.Results:Undiluted 10 per cent Betadine (n = 6) decreased the proportion of actively beating cilia over 1 minute (p < 0.01). Ciliary beat frequency decreased from 11.15 ± 4.64 Hz to no detectable activity. The result was similar with 5 per cent Betadine (n = 7), with no significant difference compared with the 10 per cent solution findings.Conclusion:Betadine, at either 5 and 10 per cent, was ciliotoxic. Caution should be applied to the use of topical Betadine solution on the respiratory mucosal surface.

Nature of the mammalian ciliary metachronal wave

1993 ◽  
Vol 75 (1) ◽  
pp. 458-467 ◽  
Author(s):  
L. B. Wong ◽  
I. F. Miller ◽  
D. B. Yeates
Keyword(s):  
Beat Frequency ◽  
Longitudinal Direction ◽  
Longitudinal Axis ◽  
Ciliary Beat Frequency ◽  
Metachronal Wave ◽  
Epithelial Tissues ◽  
Tracheal Epithelial ◽  
The Mean ◽  
Temporal And Spatial ◽  
Ciliary Beat

The temporal and spatial coordination of ciliary beat (metachronicity) is fundamental to effective mucociliary transport. Metachronal wave period (MWP) and ciliary beat frequency (CBF) of fresh excised sheep and canine tracheal epithelial tissues were measured with the use of a newly developed alternating focal spot laser light scattering system. MWP was determined from cross correlation of the heterodyne signals from the alternating focal spots. CBF was determined by autocorrelation of the heterodyne signals from each of the spots. MWP and CBF were measured in four sheep tracheal epithelial tissues with the use of longitudinal interfocal spot distances of 6 and 18 microns. In three canine tracheal epithelial tissues MWP and CBF were measured both longitudinally and circumferentially with interfocal spot distances of 5, 15, 65, 87, and 96 microns. For the sheep tracheal epithelial tissues the mean CBF was 5.9 +/- 0.4 Hz (mean of means; range 3.6 +/- 0.5 to 9.9 +/- 1.5 Hz), whereas the mean MWPs for 6- and 18-microns interfocal spot distances were 0.50 +/- 0.1 and 0.47 +/- 0.1 s, respectively. For the canine tracheal epithelial tissues the mean CBF was 4.0 +/- 0.2 Hz (2.0 +/- 0.8 to 7.2 +/- 3.2 Hz), whereas the mean longitudinal MWP was 1.5 s and the mean circumferential MWP was 2.1 s. Geometric combination of the MWP components leads to a derived MWP of 2.6 s with a propagation direction of 54 degrees with respect to the longitudinal axis of the trachea. MWP was found to be episode modulated with 12- to 20-min intervals in the longitudinal direction, but modulation was not as apparent in the circumferential direction. These data suggest that MWP and CBF are regulated by separate intracellular, intercellular, and intraciliary mechanisms.

Erythromycin Inhibits ATP-Induced Intracellular Calcium Responses in Bovine Tracheal Epithelial Cells

1998 ◽  
Vol 19 (5) ◽  
pp. 799-804 ◽  
Author(s):  
Mitsuko Kondo ◽  
Soichiro Kanoh ◽  
Jun Tamaoki ◽  
Hideki Shirakawa ◽  
Shunichi Miyazaki ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial
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