scholarly journals Charge movement and transcription regulation of L‐type calcium channel α 1S in skeletal muscle cells

2002 ◽  
Vol 540 (2) ◽  
pp. 397-409 ◽  
Author(s):  
Zhenlin Zheng ◽  
Zhong‐Min Wang ◽  
Osvaldo Delbono
Keyword(s):  
Skeletal Muscle ◽  
Calcium Channel ◽  
Transcription Regulation ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Charge Movement

scholarly journals Loss of caveolin-3 induced by the dystrophy-associated P104L mutation impairs L-type calcium channel function in mouse skeletal muscle cells

2007 ◽  
Vol 580 (3) ◽  
pp. 745-754 ◽  
Author(s):  
Harold Couchoux ◽  
Bruno Allard ◽  
Claude Legrand ◽  
Vincent Jacquemond ◽  
Christine Berthier
Keyword(s):  
Skeletal Muscle ◽  
Calcium Channel ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Mouse Skeletal Muscle ◽  
Channel Function

scholarly journals Antisense oligonucleotides against ‘cardiac’ and ‘skeletal’ DHP-receptors reveal a dual role for the ‘skeletal’ isoform in EC coupling of skeletal muscle cells in primary culture

1998 ◽  
Vol 111 (15) ◽  
pp. 2149-2158 ◽  
Author(s):  
L. Bulteau ◽  
G. Raymond ◽  
C. Cognard
Keyword(s):  
Skeletal Muscle ◽  
Membrane Potential ◽  
Calcium Channel ◽  
Primary Culture ◽  
Muscle Cells ◽  
Dihydropyridine Receptor ◽  
Skeletal Muscle Cells ◽  
Mrna Isoforms ◽  
Ec Coupling

Two dihydropyridine receptor mRNA isoforms (cardiac and skeletal) are expressed in rat skeletal muscle cells in primary culture. The progressive changes in excitation-contraction coupling mode from dual mode (‘skeletal’ and ‘cardiac’) to predominant ‘skeletal’ one during in vitro myogenesis are thought to be linked to the developmental changes in the relative expression of the two types of molecular entity previously observed in this preparation. In order to test this hypothesis, myotube cultures (5- to 7-day-old) were treated with antisense phosphorothioated oligodeoxynucleotides against cardiac or skeletal alpha1 subunit of L-type calcium channel. The oligodeoxynucleotide uptake by cells was checked by means of imaging of fluorescent oligodeoxynucleotide derivatives within the cells. Optimum concentration used (10 microM in the extracellular medium) and incubation time (70 hours) were empirically determined. Antisense directed against the cardiac type led to a 54% decrease in the averaged L-type calcium current peak density at −10 mV. The same type of experiment was performed with antisense against the skeletal isoform and led to a same order of inhibition (46%). This result clearly shows that the two isoforms can work as a calcium channel. Conversely, analysis of the shape of T-V (relative contractile amplitude versus membrane potential) curves shows that the treatment with ‘skeletal’ antisense depressed the contractile response in the medium membrane potential range whereas treatment with ‘cardiac’ antisense had no effect. This and other results taken together suggest that the skeletal isoform of dihydropyridine receptor is involved in both ‘cardiac’ and ‘skeletal’ types of EC coupling mechanisms at work in early stages of myotubes in vitro development. The type of coupling probably depends on the proximity of the skeletal dihydropyridine receptor and the ryanodine receptor.

Skeletal Muscle Cells Express Different Isoforms of the Calcium Channel α2/δ Subunit

2005 ◽  
Vol 42 (1) ◽  
pp. 013-020 ◽  
Author(s):  
Thomas Nabhani ◽  
Tanvi Shah ◽  
Jesús García
Keyword(s):  
Skeletal Muscle ◽  
Calcium Channel ◽  
Muscle Cells ◽  
Skeletal Muscle Cells
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