scholarly journals NO donors potentiate the β‐adrenergic stimulation of I Ca,L and the muscarinic activation of I K,ACh in rat cardiac myocytes

2002 ◽  
Vol 540 (2) ◽  
pp. 411-424 ◽  
Author(s):  
Najah Abi‐Gerges ◽  
Gabor Szabo ◽  
Angela S. Otero ◽  
Rodolphe Fischmeister ◽  
Pierre‐François Méry
Keyword(s):  
Cardiac Myocytes ◽  
Adrenergic Stimulation ◽  
No Donors ◽  
Rat Cardiac Myocytes ◽  
Stimulation Of

scholarly journals Na+-K+pump cycle during β-adrenergic stimulation of adult rat cardiac myocytes

1998 ◽  
Vol 507 (2) ◽  
pp. 527-539 ◽  
Author(s):  
Maxim Dobretsov ◽  
Stephanie L. Hastings ◽  
Joseph R. Stimers
Keyword(s):  
Cardiac Myocytes ◽  
Adrenergic Stimulation ◽  
Adult Rat ◽  
Rat Cardiac Myocytes ◽  
Stimulation Of

scholarly journals Adrenergic stimulation of substrate utilization by cardiac myocytes isolated from rainbow trout

1991 ◽  
Vol 159 (1) ◽  
pp. 185-202 ◽  
Author(s):  
C. L. Milligan
Keyword(s):  
Rainbow Trout ◽  
Lactate Dehydrogenase ◽  
Cardiac Myocytes ◽  
Trypan Blue ◽  
Glycogen Content ◽  
Substrate Utilization ◽  
Co2 Production ◽  
Adrenergic Agonists ◽  
Adrenergic Stimulation ◽  
Stimulation Of

A method is described for the isolation of calcium-tolerant myocytes from adult rainbow trout. Isolated myocytes remain viable for at least 4 h in suspension as indicated by (1) maintenance of ATP, phosphocreatine (PCr) and glycogen levels; (2) maintenance of the integrity of cell membranes, shown by low rates of leakage of lactate dehydrogenase (LDH) to the medium and exclusion of Trypan Blue; (3) the ability to metabolize substrates; and (4) sensitivity to adrenergic agonists. CO2 production from both glucose and lactate was sensitive to adrenergic stimulation, with the following order of potency: isoproterenol greater than noradrenaline much greater than adrenaline greater than phenylephrine, which indicates the presence of beta 1-adrenoceptors. Myocytes isolated from trout acclimated to 20 degrees C in the summer were more sensitive to beta-adrenergic stimulation than myocytes isolated from trout acclimated to 9 degrees C in either summer or winter. In the absence of exogenous fuel, there was a net reduction in myocyte glycogen content and glycogenolysis was further stimulated by 10(−7) mmol l-1 noradrenaline. However, in the presence of exogenous fuel (either 5 mmol l-1 lactate or 5 mmol l-1 glucose), glycogen was ‘spared’ and noradrenaline-stimulated glycogenolysis was apparently inhibited.

scholarly journals Stimulation of anion exchange in hypoxic rat cardiac myocytes.

1999 ◽  
Vol 79 ◽  
pp. 66
Author(s):  
Hiroshi Kawasaki ◽  
Hitomi Otani ◽  
Katsuyuki Mishima ◽  
Chiyoko Inagaki
Keyword(s):  
Anion Exchange ◽  
Cardiac Myocytes ◽  
Rat Cardiac Myocytes ◽  
Stimulation Of
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