Simultaneous measurement and imaging of intracellular Ca2+ and H+ transport in isolated rabbit gastric glands

2001 ◽  
Vol 537 (3) ◽  
pp. 735-745 ◽  
Author(s):  
J. F. Perez ◽  
M. C. Ruiz ◽  
F. Michelangeli
Keyword(s):  
Simultaneous Measurement ◽  
Gastric Glands

Simultaneous Measurement of Bend Direction and Degree of Curvature using Micromachined Grating Fibers

2014 ◽  
Vol 134 (3) ◽  
pp. 41-46
Author(s):  
Hironori Kumazaki ◽  
Munehiro Hiramatsu ◽  
Hisakazu Oguri ◽  
Seiki Inaba ◽  
Kazuhiro Hane
Keyword(s):  
Simultaneous Measurement

Simultaneous measurement of atmospheric moisture and temperature in the presence of suspended particulates using ultrasonic technique

2020 ◽  
Vol 59 (9) ◽  
pp. 096503
Author(s):  
Nairit Das ◽  
Neha Bhattacharyya ◽  
Soumendra Singh ◽  
Animesh Halder ◽  
Deep Shikha ◽  
...  
Keyword(s):  
Simultaneous Measurement ◽  
Ultrasonic Technique ◽  
Atmospheric Moisture ◽  
Suspended Particulates

Simultaneous measurement of two major prostacyclin metabolites in urine

1990 ◽  
Vol 36 (11) ◽  
pp. 1978-1980 ◽  
Author(s):  
S Zureick ◽  
J Nadler ◽  
J Yamamoto ◽  
R Horton
Keyword(s):  
Angiotensin Ii ◽  
Blood Vessels ◽  
Simultaneous Measurement ◽  
Healthy Subjects ◽  
High Accuracy ◽  
High Concentration ◽  
Accuracy And Precision ◽  
Overall Evaluation ◽  
Excretion Rates ◽  
Apparently Healthy

Abstract We describe a combined HPLC-RIA technique to measure both major metabolites of prostacyclin (PGI2): 6-keto PGF1 alpha and 2,3-dinor-6 keto PGF1 alpha. The measurement of the former, which originates from renal blood vessels, and the latter, from systemic vessels and the liver, may provide a better overall evaluation of production than measurement of one metabolite. An aliquot of acidified urine with added 3H-labeled metabolites is adsorbed and then eluted from a C18 Bond-Elut column. The sample is then passed through an HPLC system by use of an isocratic solvent combination that separates the two metabolites from known prostaglandins. The purified metabolites are then quantified by RIA. Using a logit-log10 transform, one can measure between 12 and 250 pg of either metabolite, with high accuracy and precision (CVs of 12% for a low concentration and 7% for a high concentration). Reference values for apparently healthy subjects were, respectively, 107 (SD 45) and 171 (SD 69) ng/g creatinine for 6-keto PGF1 alpha and the dinor metabolite in men (n = 18) and 45 (SD 22) and 141 (SD 28) ng/g creatinine, respectively, in women (n = 15). Indomethacin in standard doses reduced both metabolite values by 50%. Intravenous administration of angiotensin II (5 ng/kg of body wt per minute) did not alter excretion rates, but equipressor doses of norepinephrine (0.1 microgram/kg per minute) increased the production of both metabolites (6-keto greater than dinor).

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