scholarly journals Effects of reduced muscle glycogen concentration on force, Ca2+ release and contractile protein function in intact mouse skeletal muscle.

1997 ◽  
Vol 498 (1) ◽  
pp. 17-29 ◽  
Author(s):  
E R Chin ◽  
D G Allen
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Protein Function ◽  
Contractile Protein ◽  
Intact Mouse ◽  
Glycogen Concentration ◽  
Mouse Skeletal Muscle ◽  
Muscle Glycogen Concentration

scholarly journals Mechanism linking glycogen concentration and glycogenolytic rate in perfused contracting rat skeletal muscle

1992 ◽  
Vol 284 (3) ◽  
pp. 777-780 ◽  
Author(s):  
P Hespel ◽  
E A Richter
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Rat Skeletal Muscle ◽  
Phosphorylase Activity ◽  
Tension Development ◽  
Glycogen Concentration ◽  
The Difference ◽  
Resting Muscle ◽  
Muscle Glycogen Concentration ◽  
Glycogen Breakdown

The influence of differences in glycogen concentration on glycogen breakdown and on phosphorylase activity was investigated in perfused contracting rat skeletal muscle. The rats were preconditioned by a combination of swimming exercise and diet (carbohydrate-free or carbohydrate-rich) in order to obtain four sub-groups of rats with varying resting muscle glycogen concentrations (range 10-60 mumol/g wet wt.). Pre-contraction muscle glycogen concentration was closely positively correlated with glycogen breakdown over 15 min of intermittent short tetanic contractions (r = 0.75; P less than 0.001; n = 56) at the same tension development and oxygen uptake. Additional studies in supercompensated and glycogen-depleted hindquarters during electrical stimulation for 20 s or 2 min revealed that the difference in glycogenolytic rate was found at the beginning rather than at the end of the contraction period. Phosphorylase alpha activity was approximately twice as high (P less than 0.001) in supercompensated muscles as in glycogen-depleted muscles after 20 s as well as after 2 min of contractions. It is concluded that glycogen concentration is an important determinant of phosphorylase activity in contracting skeletal muscle, and probably via this mechanism a regulator of glycogenolytic rate during muscle contraction.

Skeletal muscle glycogen content: diurnal variation and effects of fasting

1976 ◽  
Vol 231 (2) ◽  
pp. 614-618 ◽  
Author(s):  
RK Conlee ◽  
MJ Rennie ◽  
WW Winder
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Diurnal Variation ◽  
Muscle Glycogen ◽  
Glycogen Content ◽  
Vastus Lateralis ◽  
Glycogen Concentration ◽  
Skeletal Muscle Glycogen ◽  
Ad Libitum ◽  
Muscle Glycogen Concentration

To test whether skeletal muscle glycogen concentration is related to food consumption, glycogen content was determined in red (R) and white (W) vastus lateralis and in soleus (S) muscles from six groups of ad libitum-fed rats killed at 4-h intervals and from 24-h-fasted animals killed at 0800 and 1600 h. The animal quarters were illuminated between 0700 and 1900 h. Glycogen values exhibited a peak at 0800 h and a nadir at 2000 h. These changes bore no relationship to blood glucose and lactate or plasma free fatty acids, glucagon, insulin, and corticosterone concentrations. Fasting resulted in reductions of glycogen content of 49% (S), 47% (R), and 29% (W) in animals killed at 0800h, but at 1600h changes were only 23% (RY), 17% (W), and 8% (S). The smaller changes at 1600 h were apparently due to lower glycogen levels in the tissues of the fed animals. It was concluded that skeletal muscle exhibits a diurnal variation in glycogen content, and that, contrary to accepted belief, fating significantly alters muscle glycogen concentration.

Role of glycogen concentration and epinephrine on glucose uptake in rat epitrochlearis muscle

1997 ◽  
Vol 272 (4) ◽  
pp. E649-E655 ◽  
Author(s):  
J. Jensen ◽  
R. Aslesen ◽  
J. L. Ivy ◽  
O. Brors
Keyword(s):  
Glucose Uptake ◽  
Muscle Glycogen ◽  
Similar Treatment ◽  
Glycogen Concentration ◽  
Spearman's Rho ◽  
Spearman’S Rho ◽  
Tracer Amount ◽  
Muscle Glycogen Concentration ◽  
Basal Glucose Uptake

The effects of diet-manipulated variations in muscle glycogen concentration and epinephrine on glucose uptake were studied in epitrochlearis muscles from Wistar rats. Both basal and insulin-stimulated glucose uptake [measured with a tracer amount of 2-[1,2-3H(N)]deoxy-D-glucose] inversely correlated with initial glycogen concentration (glycogen concentration vs. basal glucose uptake: Spearman's rho = -0.76, n = 84, P < 0.000001; glycogen concentration vs. insulin-stimulated glucose uptake: Spearman's rho = -0.67, n = 44, P < 0.00001). Two fasting-refeeding procedures were used that resulted in differences in muscle glycogen concentrations, although with similar treatment for the last 48 h before the experiment. In the rats with the lower glycogen concentration, basal as well as insulin-stimulated glucose uptake was elevated. The muscle glycogen concentration had no effect on epinephrine-stimulated glycogenolysis. Epinephrine, however, was found to reduce basal glucose uptake in all groups. These results suggest that 1) the glycogen concentration participates in the regulation of both basal and insulin-stimulated glucose uptake in skeletal muscle, 2) the magnitude of epinephrine-stimulated glycogen breakdown is independent of the glycogen concentration, and 3) epinephrine inhibits basal glucose uptake at all glycogen concentrations.

Muscle glycogen availability and temperature regulation in humans

1989 ◽  
Vol 66 (1) ◽  
pp. 72-78 ◽  
Author(s):  
L. Martineau ◽  
I. Jacobs
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Temperature Regulation ◽  
Vastus Lateralis ◽  
Water Immersion ◽  
Vastus Lateralis Muscle ◽  
Glycogen Concentration ◽  
Exercise Regimen ◽  
Before And After ◽  
Muscle Groups

The effects of intramuscular glycogen availability on human temperature regulation were studied in eight seminude subjects immersed in 18 degrees C water for 90 min or until rectal temperature (Tre) decreased to 35.5 degrees C. Each subject was immersed three times over a 3-wk period. Each immersion followed 2.5 days of a specific dietary and/or exercise regimen designed to elicit low (L), normal (N), or high (H) glycogen levels in large skeletal muscle groups. Muscle glycogen concentration was determined in biopsies taken from the vastus lateralis muscle before and after each immersion. Intramuscular glycogen concentration before the immersion was significantly different among the L, N, and H trials (P less than 0.01), averaging 247 +/- 15, 406 +/- 23, and 548 +/- 42 (SE) mmol glucose units.kg dry muscle-1, respectively. The calculated metabolic heat production during the first 30 min of immersion was significantly lower during L compared with N or H (P less than 0.05). The rate at which Tre decreased was more rapid during the L immersion than either N or H (P less than 0.05), and the time during the immersion at which Tre first began to decrease also appeared sooner during L than N or H. The results suggest that low skeletal muscle glycogen levels are associated with more rapid body cooling during water immersion in humans. Higher than normal muscle glycogen levels, however, do not increase cold tolerance.

Muscle glycogen concentrations in commercial consignments of Australian lamb measured on farm and post-slaughter after three different lairage periods

2005 ◽  
Vol 45 (5) ◽  
pp. 543 ◽  
Author(s):  
R. H. Jacob ◽  
D. W. Pethick ◽  
H. M. Chapman
Keyword(s):  
Western Australia ◽  
Muscle Glycogen ◽  
Glycogen Concentration ◽  
Age Class ◽  
Muscle Types ◽  
Carry Over ◽  
On Farm ◽  
Muscle Glycogen Concentration

The aim of this study was to gain an understanding of the distribution of glycogen concentrations and ultimate pH (pHu) in 2 different muscle types for lambs slaughtered under commercial conditions in Western Australia, and to compare muscle glycogen concentrations in lambs on farm and after slaughter. The study included 13 different consignments of prime lambs from a range of commercial scenarios. In each consignment, muscle glycogen concentration was measured in a group of lambs on farm and subsequently after slaughter in 3 different lairage groups. The lairage groups were: slaughter on arrival (no lairage), slaughter after 1 day, and slaughter after 2 days in lairage. Biopsies of M. semimembranosus and the M. semitendinosus were taken from live lambs on farm just before farm curfew before transport and from carcasses immediately after slaughter. There was a significant effect of consignment on muscle glycogen concentration. Muscle glycogen concentrations on farm were lower than 1 g/100 g in 4 consignments for the M. semimembranosus and 11 consignments for the M. semitendinosus. The cause of the differences between consignments was unclear as nutrition, genotype and age class were confounded between consignments. Glycogen concentrations were lower and meat pHu higher for sucker lamb compared with carry-over lamb consignments. However, lambs finished on grain-based feedlot rations had higher muscle glycogen concentrations than lambs finished on pasture and sucker lambs when finished on pastures only. Sucker lambs were only crossbred while carry-over lambs included crossbred and Merino genotypes. When data from different consignments were pooled and the effect of consignment was considered, there were no differences between muscle glycogen concentration measured on farm and muscle glycogen concentration measured after slaughter. However, there were differences between sample times within individual consignments. Glycogen concentration at slaughter was different from glycogen concentration on farm in more consignments for M. semitendinosus than M. semimembranosus, suggesting a difference between consignments for the effect caused by stress. Typically, the M. semimembranosus glycogen concentration at slaughter was lower than on farm in consignments consisting of Merino genotypes that had high muscle glycogen concentrations on farm. In the consignments in which lairage time had an effect on muscle glycogen concentration, the differences were small. In some consignments a difference occurred between lairage times for pHu without any difference occurring for muscle glycogen concentration.

Persistent increase in glucose uptake by rat skeletal muscle following exercise

1981 ◽  
Vol 241 (5) ◽  
pp. C200-C203 ◽  
Author(s):  
J. L. Ivy ◽  
J. O. Holloszy
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Muscle Glycogen ◽  
Glycogen Synthesis ◽  
Exercise Stress ◽  
Rat Skeletal Muscle ◽  
Major Pathway ◽  
Glycogen Accumulation ◽  
Hindlimb Muscles ◽  
Muscle Glycogen Concentration

The effect of a bout of exercise on glucose uptake and glycogen synthesis in skeletal muscle was examined using a perfused rat hindlimb preparation. Rats were subjected to a bout of swimming. The exercise stress was moderate as reflected in a reduction of muscle glycogen concentration of only 50%. Glucose uptake and glycogen synthesis were measured in perfused hindlimb muscles for a 30-min period beginning approximately 60 min following the exercise. The rate of glucose uptake in the absence of insulin was 10-fold higher in hindlimbs of exercised animals than in the controls. The rate of glucose uptake was also higher in exercised than in control muscles in the presence of 50 microunits/ml or 10 mU/ml of insulin, but these differences were smaller than that found in the absence of insulin. Conversion to glycogen was the major pathway for disposal of the glucose taken up by muscle. The rate of glycogen accumulation in the exercised plantaris muscles was greater than in the control muscles both in the absence and presence of insulin.

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