scholarly journals Activation of a Cl- conductance by SCN- in single proximal tubule cells isolated from Rana temporaria.

1995 ◽  
Vol 486 (3) ◽  
pp. 715-721 ◽  
Author(s):  
L Robson ◽  
R Tarran ◽  
M Hunter
Keyword(s):  
Proximal Tubule ◽  
Rana Temporaria ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Cl Conductance

Na+-alanine uptake activates a Cl− conductance in frog renal proximal tubule cells via nonconventional PKC

2001 ◽  
Vol 280 (5) ◽  
pp. F758-F767
Author(s):  
I. D. Millar ◽  
L. Robson
Keyword(s):  
Proximal Tubule ◽  
Atp Hydrolysis ◽  
Renal Proximal Tubule ◽  
Selectivity Ratio ◽  
Proximal Tubule Cells ◽  
Kinase C ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
Pkc Activation ◽  
Cl Conductance

Hyposmotically induced swelling of frog renal proximal tubule cells activates a DIDS-sensitive, outwardly rectifying Cl−conductance via a conventional protein kinase C (PKC). This study examines whether Na+-alanine cotransport similarly activates a DIDS-sensitive Cl− conductance in frog renal proximal tubule cells. On stimulation of Na+-alanine cotransport, the DIDS-sensitive current ( I DIDS-Ala) increased markedly over time. I DIDS-Ala exhibited outward rectification, a Na+/Cl− selectivity ratio of 0.19 ± 0.03, and an anion selectivity sequence Br− = Cl− > I− > gluconate−. Activation of I DIDS-Alawas dependent on ATP hydrolysis and PKC-mediated phosphorylation and was inhibited by hyperosmotic conditions. Activation could be not ascribed to a conventional PKC isoform, as I DIDS-Ala was not affected by removing Ca2+ or by phorbol ester treatment, suggesting a role for a nonconventional PKC isoform, either novel or atypical. We conclude that Na+-alanine cotransport activates a DIDS-sensitive Cl− conductance via a nonconventional PKC isoform. This contrasts with the hyposmotically activated Cl−conductance, which requires conventional PKC activation.

Nephrotoxicity Testing In Vitro: The Current Situation

1997 ◽  
Vol 25 (5) ◽  
pp. 497-503
Author(s):  
Jean-Paul Morin ◽  
Marc E. De Broe ◽  
Walter Pfaller ◽  
Gabriele Schmuck
Keyword(s):  
Proximal Tubule ◽  
Culture Media ◽  
Task Force ◽  
Primary Cultures ◽  
Phenotypic Expression ◽  
Transepithelial Transport ◽  
Specific Cell ◽  
Proximal Tubule Cells ◽  
Tubule Cells

An ECVAM task force on nephrotoxicity has been established to advise, in particular, on the follow-up to recommendations made in the ECVAM workshop report on nephrotoxicity testing in vitro. Since this workshop was held, in 1994, there have been several improvements in the techniques used. For example, the duration of renal slice viability, and the maintenance of functional activities in slices, have been improved by using dynamic incubation systems with higher oxygen tensions and more-appropriate cell culture media. Highly differentiated primary cultures of pig, human and rabbit proximal tubule cells have been established by using specific cell isolation procedures and/or selective culture media. To date, the most comparable phenotypic expression and transepithelial transport capacities to proximal tubules in vivo have been obtained with primary cultures of rabbit proximal tubule cells which are grown on bicompartmental supports; in this system, transepithelial substrate gradients are generated and the transepithelial transport of both organic anions and cations is highly active. This in vitro system has been selected by ECVAM for further evaluation and prevalidation. Industrial needs in the area of nephrotoxicity testing have been identified, and recommendations are made at the end of this report concerning possible future initiatives.

Effects of TCDD and estradiol-17β on the proliferation and Na+/glucose cotransporter in renal proximal tubule cells

2005 ◽  
Vol 19 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Ho Jae Han ◽  
Min Jin Lim ◽  
Yun Jung Lee ◽  
Eun Jung Kim ◽  
Young Jin Jeon ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
Estradiol 17Β
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