scholarly journals Interconversion between distinct gating pathways of the high threshold calcium channel in rat ventricular myocytes.

1993 ◽  
Vol 462 (1) ◽  
pp. 197-228 ◽  
Author(s):  
S Richard ◽  
P Charnet ◽  
J M Nerbonne
Keyword(s):  
Calcium Channel ◽  
Ventricular Myocytes ◽  
High Threshold ◽  
Rat Ventricular Myocytes

Changes of action potential and L-type calcium channel current of Sprague–Dawley rat ventricular myocytes by different amlodipine isomers

2008 ◽  
Vol 86 (9) ◽  
pp. 620-625 ◽  
Author(s):  
Ru-xing Wang ◽  
Wen-ping Jiang
Keyword(s):  
Steady State ◽  
Action Potential ◽  
Calcium Channel ◽  
Ventricular Myocytes ◽  
Channel Blockers ◽  
Sprague Dawley ◽  
Patch Clamp Technique ◽  
Rat Ventricular Myocytes ◽  
Channel Current ◽  
Steady State Inactivation

To investigate the effects of S- and R-amlodipine (Aml) on action potential (AP) and L-type calcium channel current (ICa-L), the whole-cell patch-clamp technique was used on rat ventricular myocytes to record AP, ICa-L, peak currents, steady-state activation currents, steady-state inactivation currents, and recovery currents from inactivation with S-Aml and R-Aml at various concentrations. Increasing concentrations of S-Aml gradually shortened AP durations (APDs). At concentrations of 0.1, 0.5, 1, 5, and 10 μmol/L, S-Aml blocked 1.5% ± 0.2%, 25.4% ± 5.3%, 65.2% ± 7.3%, 78.4% ± 8.1%, and 94.2% ± 5.0% of ICa-L, respectively (p < 0.05), and the half-inhibited concentration was 0.62 ± 0.12 µmol/L. Current–voltage curves were shifted upward; steady-state activation and inactivation curves were shifted to the left. At these concentrations of S-Aml, the half-activation voltages were –16.01 ± 1.65, –17.61 ± 1.60, –20.17 ± 1.46, –21.87 ± 1.69, and –24.09 ± 1.87 mV, respectively, and the slope factors were increased (p < 0.05). The half-inactivation voltages were –27.16 ± 4.48, –28.69 ± 4.52, –31.19 ± 4.17, –32.63 ± 4.34, and –35.16 ± 4.46 mV, respectively, and the slope factors were increased (p < 0.05). The recovery times from inactivation of S-Aml were prolonged (p < 0.05). In contrast, R-Aml had no effect on AP and ICa-L (p > 0.05) at the concentrations tested. Thus, only S-Aml has calcium channel blockade activity, whereas R-Aml has none of the pharmacologic actions associated with calcium channel blockers.

scholarly journals Hyposmotic challenge modulates function of L-type calcium channel in rat ventricular myocytes through protein kinase C

2010 ◽  
Vol 31 (11) ◽  
pp. 1438-1446 ◽  
Author(s):  
An-tao Luo ◽  
Hong-yan Luo ◽  
Xin-wu Hu ◽  
Lin-lin Gao ◽  
Hua-min Liang ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Calcium Channel ◽  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes ◽  
Kinase C

scholarly journals Effects of Leonurine on L-Type Calcium Channel in Rat Ventricular Myocytes

2012 ◽  
Vol 35 (8) ◽  
pp. 1249-1256 ◽  
Author(s):  
Hong Xin ◽  
Ming Gu ◽  
Wen Wei Wang ◽  
Shi Ying Huang ◽  
Fang Ping Li ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes

Gonadectomy of adult male rats reduces contractility of isolated cardiac myocytes

2003 ◽  
Vol 285 (3) ◽  
pp. E449-E453 ◽  
Author(s):  
Kish L. Golden ◽  
James D. Marsh ◽  
Yang Jiang ◽  
Tiane Brown ◽  
Jerome Moulden
Keyword(s):  
Cardiac Function ◽  
Calcium Channel ◽  
Cardiac Myocytes ◽  
Ventricular Myocytes ◽  
Functional Expression ◽  
Receptor Expression ◽  
Male Rats ◽  
Rat Ventricular Myocytes ◽  
Time To Peak ◽  
Isolated Rat

Sex-related differences in cardiac function have been well documented. The extent to which sex hormones are responsible for these differences is unclear. The current study was designed to determine whether castration and androgen replacement resulted in changes in functional expression of genes encoding the L-type calcium channel and Na/Ca exchanger in isolated rat ventricular myocytes. Sixteen weeks of castration produced a 50% decline in dihydropyridine receptor expression levels and a 16% ( P < 0.05) increase in time to peak shortening. Furthermore, cardiac myocytes isolated from castrated animals also displayed an 18% ( P < 0.001) increase in time to relengthening and an 80% decrease in Na/Ca exchanger gene expression when compared with intact controls. Testosterone treatment of castrated animals completely reversed these effects. These results provide the first evidence that androgens regulate functional expression of the L-type calcium channel and the Na/Ca exchanger in isolated rat ventricular myocytes and thus may play a role in modulating cardiac performance in males and thereby contribute to the observed gender differences in cardiac function.

scholarly journals Role of the T-Type Calcium Channel CaV3.2 in the Chronotropic Action of Corticosteroids in Isolated Rat Ventricular Myocytes

Endocrinology ◽  
2009 ◽  
Vol 150 (8) ◽  
pp. 3726-3734 ◽  
Author(s):  
Andrés Maturana ◽  
Sébastien Lenglet ◽  
Magaly Python ◽  
Shun'Ichi Kuroda ◽  
Michel F. Rossier
Keyword(s):  
Calcium Channel ◽  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes ◽  
Chronotropic Action ◽  
Isolated Rat
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