scholarly journals Effects of amiloride and ouabain on short-chain fatty acid transport in guinea-pig large intestine.

1993 ◽  
Vol 460 (1) ◽  
pp. 455-466 ◽  
Author(s):  
W von Engelhardt ◽  
M Burmester ◽  
K Hansen ◽  
G Becker ◽  
G Rechkemmer
Keyword(s):  
Fatty Acid ◽  
Guinea Pig ◽  
Large Intestine ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Fatty Acid Transport ◽  
Acid Transport

Significance of Short Chain Fatty Acid Transport by Members of the Monocarboxylate Transporter Family (MCT)

2012 ◽  
Vol 37 (11) ◽  
pp. 2562-2568 ◽  
Author(s):  
Ivano Moschen ◽  
Angelika Bröer ◽  
Sandra Galić ◽  
Florian Lang ◽  
Stefan Bröer
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Monocarboxylate Transporter ◽  
Fatty Acid Transport ◽  
Acid Transport ◽  
Transporter Family

scholarly journals Short-chain fatty acid transport by the rat colon in vitro

1978 ◽  
Vol 74 (5) ◽  
pp. 1005 ◽  
Author(s):  
S. Bar-Meir ◽  
H. Ruppin ◽  
K.H. Soergel ◽  
S. Broor ◽  
C.M. Wood
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Rat Colon ◽  
Fatty Acid Transport ◽  
Acid Transport

Short-chain fatty acid transport and its effects on ion transport by rabbit cecum

1987 ◽  
Vol 253 (2) ◽  
pp. G171-G178
Author(s):  
M. Hatch
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Short Circuit ◽  
Basolateral Membrane ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Bathing Solution ◽  
Fatty Acid Transport ◽  
Unexpected Finding

Short-chain fatty acid (SCFA) metabolism and transport were examined in vitro across isolated rabbit cecal epithelia whose primary function is absorption of these solutes. This study shows that although there was some low-level metabolism of SCFAs to ketone bodies by the isolated cecum, a significantly higher oxygen consumption was sustained for a longer time period by tissues incubated in glucose-containing salines. The cecum supported a significant net secretory flux of acetate (J net Ac- = -1.13 +/- 0.13 mu eq X cm-2 X h-1) and propionate (J net Pr- = -0.61 +/- 0.14 mu eq X cm-2 X h-1). This study also shows that glucose significantly enhanced short-circuit current (Isc), tissue conductance (Gt), and sodium transport across this tissue. Neither Ac- nor Pr- enhanced net sodium flux (J net Ac-) but Pr- significantly reduced net chloride flux (J net Cl-), whereas Ac- had no effect. The increase in Isc and Gt observed in the presence of SCFAs was attributable to the presence of SCFA in the serosal bathing solution alone. To explain the latter finding and the unexpected finding of SCFA secretion, the existence of an electrogenic anion (HCO3-) secretory pathway is postulated. It is suggested that this system can accommodate SCFAs in vitro and that it is a Na+-dependent system located on the basolateral membrane of the cecal cell.

scholarly journals Short chain fatty acid absorption by the human large intestine.

Gut ◽  
1978 ◽  
Vol 19 (9) ◽  
pp. 819-822 ◽  
Author(s):  
N I McNeil ◽  
J H Cummings ◽  
W P James
Keyword(s):  
Fatty Acid ◽  
Large Intestine ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Acid Absorption ◽  
Human Large Intestine ◽  
Fatty Acid Absorption

Transport of propionate by human ileal brush-border membrane vesicles

1991 ◽  
Vol 260 (5) ◽  
pp. G776-G782 ◽  
Author(s):  
J. M. Harig ◽  
K. H. Soergel ◽  
J. A. Barry ◽  
K. Ramaswamy
Keyword(s):  
Fatty Acid ◽  
Anion Exchange ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Short Chain Fatty Acid ◽  
Membrane Vesicles ◽  
Chain Fatty Acid ◽  
Brush Border Membrane Vesicles ◽  
Short Chain ◽  
Fatty Acid Transport

Human ileal brush-border membrane vesicles were employed to study the mechanisms of short-chain fatty acid (propionate) absorption especially to determine the effects of intravesicular HCO3- and the component of nonionic diffusion. Preloading the vesicles with HCO3- resulted in up to 20-fold "overshoots" of transport, and this effect was not seen with other intravesicular anions. This transport process was very fast (peak uptake 6 s) and was not due to intravesicular buffering by HCO3-. Radiolabeled propionate transport demonstrated transstimulation when the vesicles were preloaded with unlabeled propionate. An inward H+ gradient led to stimulation of propionate transport much smaller than in the presence of trans-HCO3-, whereas an inward Na+ gradient had no effect. Propionate transport was attenuated by the anion exchange inhibitors SITS and DIDS. Under HCO3- gradient conditions, propionate transport exhibited saturation kinetics with an apparent Km of 21 +/- 3 mM and a Vmax of 50 +/- 3 nmol.mg protein-1.3 s-1. Propionate transport was inhibited up to 40% by 2-5 carbon short-chain fatty acids (10 mM) but not by other organic anions. Short-chain fatty acid transport in the human ileum is Na+ independent and occurs mostly via a specific anion exchange mechanism with HCO3-. Our results also demonstrate a small component of nonionic diffusion of the protonated fatty acid (or anion exchange for OH-).

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