scholarly journals Characterization of membrane currents in single smooth muscle cells from the guinea-pig gastric antrum.

1992 ◽  
Vol 451 (1) ◽  
pp. 387-417 ◽  
Author(s):  
T Noack ◽  
P Deitmer ◽  
E Lammel
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Membrane Currents ◽  
Gastric Antrum

Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

1997 ◽  
Vol 136 (5) ◽  
pp. 531-538 ◽  
Author(s):  
Dessislava B Duridanova ◽  
Milena D Nedelcheva ◽  
Hristo S Gagov
Keyword(s):  
Smooth Muscle ◽  
Cell Membrane ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Gastric Antrum ◽  
Dependent Manner ◽  
Response Curves ◽  
Dose Dependent ◽  
In Cells

Abstract To study the effects of oxytocin on both spontaneous phasic contractions and K+ outward currents (IK) of the so-called 'non-target' smooth muscle cells, physiological concentrations of oxytocin ranging between 10−12 mol/l and 10−8 mol/l were applied to smooth muscle preparations and single voltage-clamped cells isolated from the circular layer of the guinea-pig gastric antrum. Oxytocin (10−12mol/l to 10−8 mol/l) suppressed, in a dose-dependent manner, the tetrodotoxin- and atropine-resistant spontaneous phasic contractions and shifted rightward the dose–response curves of 10−7 mol/l charybdotoxin and 10−3mol/l BaCl2. In cells with preloaded intracellular Ca2+ stores, oxytocin (10−12 mol/l to 10−9 mol/l) caused a dose-dependent activation of the charybdotoxin-blockable non-inactivating component of IK (IK(s1)) of single voltage-clamped cells, which was accompanied by hyperpolarization of the cell membranes. 8Lys-vasopressin and 8arg-vasopressin failed to mimic the effects of oxytocin on both contraction and K+ currents. Further, the oxytocin-induced activation of IK(s1) was effectively antagonized by 5× 10−8 mol/l U-73122 or 5× 10−6 mol/l 2-nitro-4-carboxyphenyl N,N-diphenylcarbamate (inhibitors of the cell membrane phospholipase C), as well as by intracellularly applied heparin (selective inhibitor of inositol-1,4,5-trisphosphate (IP3)-induced Ca2+ release channels). In cells incubated in the absence of Ca2+ entry throughout the study, oxytocin (10−9 mol/l) caused a slight and transient increase of IK(s1) amplitudes. Neither ryanodine (10−6 mol/l nor cyclopiazonic acid (10−6 mol/l) were able to restore the IK-activating effect of oxytocin in these cells. The data obtained suggest (i) that selective oxytocin receptors are present on the membranes of guinea-pig antral smooth muscle cells, (ii) that the oxytocin-related relaxation may result from the activation of Ca2+-sensitive K+ conductivity via activation of IP3-induced release of Ca from the submembrane located cisternae of the sarcoplasmic reticulum Ca2+ stores and (iii) in turn, this evokes a non-inactivating component of IK, hyperpolarizing the cell membrane. European Journal of Endocrinology 136 531–538

scholarly journals Effects of KRN2391 on membrane currents in tracheal smooth muscle cells of the guinea-pig

1997 ◽  
Vol 73 ◽  
pp. 257
Author(s):  
Yuichiro Matsushita ◽  
Katsuhiko Muraki ◽  
Yun Imaizumi ◽  
Minoru Watanabe
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Membrane Currents ◽  
Tracheal Smooth Muscle

Characterization of Spontaneous Depolarizations in Smooth Muscle Cells of the Guinea Pig Prostate

2006 ◽  
Vol 175 (1) ◽  
pp. 370-380 ◽  
Author(s):  
Richard J. Lang ◽  
Dan-Thanh T. Nguyen ◽  
Hayato Matsuyama ◽  
Tadashi Takewaki ◽  
Betty Exintaris
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells
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