scholarly journals Spontaneous sarcoplasmic reticulum calcium release and extrusion from bovine, not porcine, coronary artery smooth muscle.

1992 ◽  
Vol 451 (1) ◽  
pp. 49-78 ◽  
Author(s):  
L Stehno-Bittel ◽  
M Sturek
Keyword(s):  
Smooth Muscle ◽  
Coronary Artery ◽  
Sarcoplasmic Reticulum ◽  
Calcium Release ◽  
Porcine Coronary Artery ◽  
Sarcoplasmic Reticulum Calcium

Cyclic ADP-ribose stimulates sarcoplasmic reticulum calcium release in porcine coronary artery smooth muscle

1996 ◽  
Vol 270 (2) ◽  
pp. H801-H806 ◽  
Author(s):  
M. S. Kannan ◽  
A. M. Fenton ◽  
Y. S. Prakash ◽  
G. C. Sieck
Keyword(s):  
Smooth Muscle ◽  
Coronary Artery ◽  
Sarcoplasmic Reticulum ◽  
Calcium Release ◽  
Direct Evidence ◽  
Ryanodine Receptors ◽  
Porcine Coronary Artery ◽  
Inositol 1 ◽  
Cyclic Adp Ribose ◽  
Sarcoplasmic Reticulum Calcium

Cyclic ADP-ribose (cADPR) was shown to induce calcium release from the endoplasmic reticulum via ryanodine-sensitive pathways. In smooth muscle, two pathways for calcium release from the sarcoplasmic reticulum (SR) have been previously demonstrated: D-myo-inositol 1, 4, 5-trisphosphate-gated and ryanodine-gated. However, evidence for cADPR as a regulator for SR Ca2+ release in smooth muscle is lacking. We used permeabilized porcine coronary artery smooth muscle cells to directly examine the stimulation of SR Ca2+ release by cADPR. The results provide direct evidence that cADPR stimulates SR Ca2+ release and that this response is not inhibited by heparin, by depletion of the caffeine-sensitive Ca2+ pool, or by blockade or ryanodine receptors. These results indicate a novel mechanism for Ca2+ release from the SR of vascular smooth muscle.

Exercise training alters Ca release from coronary smooth muscle sarcoplasmic reticulum

1990 ◽  
Vol 259 (2) ◽  
pp. H643-H647 ◽  
Author(s):  
L. Stehno-Bittel ◽  
M. H. Laughlin ◽  
M. Sturek
Keyword(s):  
Smooth Muscle ◽  
Coronary Artery ◽  
Sarcoplasmic Reticulum ◽  
Exercise Training ◽  
Recovery Time ◽  
Recovery Period ◽  
Oxidative Capacity ◽  
Free Calcium ◽  
Coronary Smooth Muscle ◽  
Porcine Coronary Artery

We examined intracellular free calcium (Cai) buffering by the sarcoplasmic reticulum (SR) of porcine coronary artery smooth muscle cells (SMC) and the effects of chronic exercise training (ET) on this phenomenon. Cai was measured with fura-2. Depolarization of single SMC via high extracellular K induced the entry of Ca, a fraction of which was sequestered (buffered) by the SR. Endurance ET of Yucatan minipigs for 16-20 wk increased exercise tolerance, heart-to-body weight ratios, and skeletal muscle oxidative capacity. Caffeine-induced Ca release from the SR after a short (2 min) recovery time from depolarization to caffeine exposure was equal in SMC from both ET and sedentary pigs. With a longer (11 min) recovery period after depolarization, caffeine-induced Ca release from the SR of sedentary pigs was equal to that after the short recovery time. However, after the long recovery, caffeine-induced Ca release in SMC of ET pigs was 60% less (P less than 0.05) than in the other protocols. These results indicate that chronic ET increased Cai buffering in SMC of the coronary artery by increasing the rate of spontaneous Ca extrusion from the SR.

scholarly journals Cardiac Sarcoplasmic Reticulum Calcium Release and Load Are Enhanced by Subcellular cAMP Elevations in PI3Kγ-Deficient Mice

2005 ◽  
Vol 96 (10) ◽  
pp. 1079-1086 ◽  
Author(s):  
Benoit-Gilles Kerfant ◽  
Dominica Gidrewicz ◽  
Hui Sun ◽  
Gavin Y. Oudit ◽  
Josef M. Penninger ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Release ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Sarcoplasmic Reticulum Calcium ◽  
Deficient Mice
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