scholarly journals Substance P inhibits activation of calcium-dependent potassium conductances in guinea-pig myenteric neurones.

1992 ◽  
Vol 447 (1) ◽  
pp. 293-308 ◽  
Author(s):  
K Morita ◽  
Y Katayama
Keyword(s):  
Substance P ◽  
Guinea Pig ◽  
Calcium Dependent ◽  
Potassium Conductances ◽  
Myenteric Neurones

Potassium currents and their modulation by muscarine and substance P in neuronal cultures from adult guinea pig celiac ganglia

1993 ◽  
Vol 69 (5) ◽  
pp. 1632-1644 ◽  
Author(s):  
S. Vanner ◽  
R. J. Evans ◽  
S. G. Matsumoto ◽  
A. Surprenant
Keyword(s):  
Substance P ◽  
Guinea Pig ◽  
Action Potential ◽  
Potassium Current ◽  
Potassium Currents ◽  
Resting Potential ◽  
Time Constants ◽  
Whole Cell ◽  
Calcium Dependent ◽  
Ganglion Neurons

1. Intracellular microelectrode and whole-cell patch-clamp recordings were obtained from adult guinea pig celiac ganglion neurons grown in tissue culture for 7-14 days. Over 90% of neurons showed phasic-type action-potential discharge with the use of either type of recording electrode; they stained immunohistochemically for catecholamines, tyrosine hydroxylase, and neuropeptide Y. Input resistance (140 M omega) and action-potential amplitude (103 mV) were significantly greater with whole-cell than with microelectrode recordings, but other passive electrical properties were similar. 2. Five potassium currents were characterized: an apamin-sensitive after hyperpolarizing current (IAHP), an apamin and tetraethylammonium-insensitive slow IAHP, an M-like current, a transient outward IA current, and a delayed rectifier IK current. A hyperpolarization-activated cationic Ih current was also present. The first three currents were not observed with whole-cell recordings. 3. Cadmium (200 microM), cobalt (1 mM), lanthanum (30 microM), or a low calcium/high magnesium solution blocked both IAHPS and the M-like current; barium (1 mM) also blocked these currents. 4. Kinetics of the M-like current were best described by a double exponential fit to deactivating tail currents with time constants of 50 and 390 ms at -50 mV. The apamin-sensitive and slow IAHP decayed exponentially with time constants of 145 ms and 3.5 s, respectively. There was no correlation between occurrence of M-like current (95% of neurons) and slow IAHP (40% of neurons), nor any correlation between magnitude of M-like current and IAHP in those cells exhibiting both currents. 5. Muscarine and substance P (SP) caused depolarizations or inward currents (under voltage clamp) at the resting potential (-55 mV) associated with a decreased membrane conductance. The slow IAHP and the M-like current, but not the apamin-sensitive IAHP nor the IA, were blocked by muscarine and SP (IC50 3 microM and 100 nM, respectively). Muscarine and SP also decreased a "leak" potassium current. 6. We conclude that celiac neurons express two calcium-dependent IAHP currents and a calcium-dependent M-current; these are seen by fine-tipped intracellular microelectrodes but not by whole-cell patch electrodes. These currents are not required for spike frequency accommodation. Muscarine and SP reduce these currents, as well as voltage-independent leakage potassium current.

Capsaicin-induced release of substance P-like immunoreactivity from the guinea-pig stomach

1988 ◽  
Vol 22 (1-2) ◽  
pp. 156
Author(s):  
D. Renzi ◽  
P. Santicioli ◽  
C.A. Maggi ◽  
C. Surrenti ◽  
A. Calabrò ◽  
...  
Keyword(s):  
Substance P ◽  
Guinea Pig ◽  
Guinea Pig Stomach

scholarly journals Substance P-induced pulmonary vasoreactivity in isolated perfused guinea pig lung.

1988 ◽  
Vol 62 (2) ◽  
pp. 196-203 ◽  
Author(s):  
W M Selig ◽  
K E Burhop ◽  
J G Garcia ◽  
A B Malik
Keyword(s):  
Substance P ◽  
Guinea Pig

Substance P stimulates inhibitory synaptic transmission in the guinea pig basolateral amygdala in vitro

2001 ◽  
Vol 40 (6) ◽  
pp. 806-817 ◽  
Author(s):  
Karen A Maubach ◽  
Karine Martin ◽  
David W Smith ◽  
Louise Hewson ◽  
Robert A Frankshun ◽  
...  
Keyword(s):  
Substance P ◽  
Guinea Pig ◽  
Synaptic Transmission ◽  
Basolateral Amygdala ◽  
Inhibitory Synaptic Transmission
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