scholarly journals On the mechanism of nucleotide diphosphate activation of the ATP-sensitive K+ channel in ventricular cell of guinea-pig.

1991 ◽  
Vol 437 (1) ◽  
pp. 239-256 ◽  
Author(s):  
R T Tung ◽  
Y Kurachi
Keyword(s):  
Guinea Pig ◽  
K Channel ◽  
Ventricular Cell

scholarly journals Large-conductance voltage- and Ca2+-activated K+ channel regulation by protein kinase C in guinea pig urinary bladder smooth muscle

2014 ◽  
Vol 306 (5) ◽  
pp. C460-C470 ◽  
Author(s):  
Kiril L. Hristov ◽  
Amy C. Smith ◽  
Shankar P. Parajuli ◽  
John Malysz ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Membrane Potential ◽  
Guinea Pig ◽  
Bk Channels ◽  
Bk Channel ◽  
K Channel ◽  
Channel Activity ◽  
Open Probability ◽  
Channel Regulation ◽  
Pkc Activation

Large-conductance voltage- and Ca2+-activated K+ (BK) channels are critical regulators of detrusor smooth muscle (DSM) excitability and contractility. PKC modulates the contraction of DSM and BK channel activity in non-DSM cells; however, the cellular mechanism regulating the PKC-BK channel interaction in DSM remains unknown. We provide a novel mechanistic insight into BK channel regulation by PKC in DSM. We used patch-clamp electrophysiology, live-cell Ca2+ imaging, and functional studies of DSM contractility to elucidate BK channel regulation by PKC at cellular and tissue levels. Voltage-clamp experiments showed that pharmacological activation of PKC with PMA inhibited the spontaneous transient BK currents in native freshly isolated guinea pig DSM cells. Current-clamp recordings revealed that PMA significantly depolarized DSM membrane potential and inhibited the spontaneous transient hyperpolarizations in DSM cells. The PMA inhibitory effects on DSM membrane potential were completely abolished by the selective BK channel inhibitor paxilline. Activation of PKC with PMA did not affect the amplitude of the voltage-step-induced whole cell steady-state BK current or the single BK channel open probability (recorded in cell-attached mode) upon inhibition of all major Ca2+ sources for BK channel activation with thapsigargin, ryanodine, and nifedipine. PKC activation with PMA elevated intracellular Ca2+ levels in DSM cells and increased spontaneous phasic and nerve-evoked contractions of DSM isolated strips. Our results support the concept that PKC activation leads to a reduction of BK channel activity in DSM via a Ca2+-dependent mechanism, thus increasing DSM contractility.

Acetylcholine response in guinea pig outer hair cells. II. Activation of a small conductance Ca2+-activated K+ channel

1996 ◽  
Vol 101 (1-2) ◽  
pp. 149-172 ◽  
Author(s):  
Anastas P. Nenov ◽  
Charles Norris ◽  
Richard P. Bobbin
Keyword(s):  
Guinea Pig ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
K Channel ◽  
Small Conductance

scholarly journals Characterization of the K+-channel-coupled adenosine receptor in guinea pig atria

1989 ◽  
Vol 340 (6) ◽  
pp. 684-688 ◽  
Author(s):  
H. Tawfik-Schlieper ◽  
K. -N. Klotz ◽  
V. A. W. Kreye ◽  
U. Schwabe
Keyword(s):  
Guinea Pig ◽  
Adenosine Receptor ◽  
K Channel ◽  
Guinea Pig Atria

K+ channel openers produce epithelium-dependent relaxation of the guinea-pig trachea

1995 ◽  
Vol 282 (1-3) ◽  
pp. 193-197 ◽  
Author(s):  
Ken-ichi Shikada ◽  
Sakuya Tanaka
Keyword(s):  
Guinea Pig ◽  
K Channel
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