scholarly journals Regulation of the intracellular free calcium concentration in single rat dorsal root ganglion neurones in vitro.

1990 ◽  
Vol 425 (1) ◽  
pp. 85-115 ◽  
Author(s):  
S A Thayer ◽  
R J Miller
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Calcium Concentration ◽  
Intracellular Free Calcium ◽  
Free Calcium ◽  
Intracellular Free Calcium Concentration ◽  
Free Calcium Concentration

scholarly journals Bryophyllum pinnatum Compounds Inhibit Oxytocin-Induced Signaling Pathways in Human Myometrial Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Stefanie Santos ◽  
Leonie Zurfluh ◽  
Mónica Mennet ◽  
Olivier Potterat ◽  
Ursula von Mandach ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Calcium Concentration ◽  
Inhibitory Effect ◽  
Intracellular Free Calcium ◽  
Free Calcium ◽  
Myometrial Cells ◽  
Intracellular Free Calcium Concentration ◽  
Free Calcium Concentration ◽  
Bryophyllum Pinnatum

Bryophyllum pinnatum has been used in the treatment of premature labor, first in anthroposophic hospitals and, recently, in conventional settings as an add-on medication. In vitro work with hTERT human myometrial cells showed that B. pinnatum leaf press juice inhibits the increase of intracellular free calcium concentration induced by oxytocin, a hormone known to play a role in labor. Our aim was to identify fractions/compounds in B. pinnatum press juice that contribute to this inhibitory effect, and to investigate their effect on oxytocin-driven activation of the MAPK cascade. Several fractions/compounds from B. pinnatum press juice led to a concentration-dependent decrease of oxytocin-induced increase of intracellular free calcium concentration, but none of them was as strong as B. pinnatum press juice. However, the combination of a bufadienolide and a flavonoid-enriched fraction was as effective as B. pinnatum press juice, and their combination had a synergistic effect. B. pinnatum press juice inhibited oxytocin-driven activation of MAPKs SAPK/JNK and ERK1/2, an effect also exerted by the bufadienolide-enriched fraction. The effect of B. pinnatum press juice on oxytocin-induced signaling pathways was comparable to that of the oxytocin-receptor antagonist and tocolytic agent atosiban. Our findings further substantiate the use of B. pinnatum press juice preparations in the treatment of preterm labor.

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