scholarly journals Effects of 2-ketoisocaproate on insulin release and single potassium channel activity in dispersed rat pancreatic beta-cells.

1987 ◽  
Vol 385 (1) ◽  
pp. 517-529 ◽  
Author(s):  
F M Ashcroft ◽  
S J Ashcroft ◽  
D E Harrison
Keyword(s):  
Potassium Channel ◽  
Beta Cells ◽  
Insulin Release ◽  
Pancreatic Beta Cells ◽  
Channel Activity ◽  
Potassium Channel Activity

scholarly journals Mechanisms of Impaired Glucose Tolerance and Insulin Secretion during Isoflurane Anesthesia

2009 ◽  
Vol 111 (5) ◽  
pp. 1044-1051 ◽  
Author(s):  
Katsuya Tanaka ◽  
Takashi Kawano ◽  
Takehito Tomino ◽  
Hiroaki Kawano ◽  
Tsuyoshi Okada ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Patch Clamp ◽  
Glucose Tolerance ◽  
Potassium Channel ◽  
Beta Cells ◽  
Mechanism Of Action ◽  
Pancreatic Beta Cells ◽  
Glucose Utilization ◽  
Channel Activity ◽  
Potassium Channel Activity

Background Volatile anesthetics impair insulin secretion and glucose utilization; however, the precise mechanism of action that underlies these effects is unknown. The authors hypothesized that isoflurane inhibits glucose-induced inhibition of adenosine triphosphate-sensitive potassium channel activity in pancreatic beta cells, which could result in impaired insulin secretion and glucose tolerance. Methods Intravenous glucose tolerance tests were performed on 28 male Japanese White rabbits anesthetized with sodium pentobarbital. Glibenclamide (50 microg/kg + 33.5 microg x kg x h) or vehicle was administered 75 min before intravenous administration of 0.6 g/kg glucose. Half of the animals (n = 7) in the vehicle and glibenclamide groups received isoflurane at 1.0 minimum alveolar concentration 30 min before administration of glucose, and the other half received a vehicle control. Hemodynamics, blood glucose, and plasma insulin were measured. A cell-attached patch clamp configuration was used to record single channel currents in the pancreas from male Swiss-Webster mice. Results Isoflurane alone or a combination of isoflurane and glibenclamide inhibited the insulinogenic index to a greater extent than in the vehicle and glibenclamide groups. In the patch clamp experiments, channel activity was significantly decreased as the glucose concentration was increased from 0 to 10 mm. The subsequent application of 0.5 mm isoflurane reversed the effects of glucose on channel activity. Conclusion These results show that isoflurane impairs insulin secretion and glucose utilization. The mechanism of action responsible for these effects may involve a decrease in glucose-induced inhibition of adenosine triphosphate-sensitive potassium channel activity in pancreatic beta cells.

Dendrotoxin-binding brain membrane protein displays a potassium channel activity that is stimulated by both cAMP-dependent and endogenous phosphorylations

Biochemistry ◽  
1989 ◽  
Vol 28 (15) ◽  
pp. 6455-6460 ◽  
Author(s):  
Hubert Rehm ◽  
Siegried Pelzer ◽  
Claude Cochet ◽  
Edmond Chambaz ◽  
Bruce L. Tempel ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Potassium Channel ◽  
Channel Activity ◽  
Brain Membrane ◽  
Potassium Channel Activity

scholarly journals Post-transcriptional Control of Human maxiK Potassium Channel Activity and Acute Oxygen Sensitivity by Chronic Hypoxia

2003 ◽  
Vol 278 (51) ◽  
pp. 51422-51432 ◽  
Author(s):  
Matthew E. Hartness ◽  
Stephen P. Brazier ◽  
Chris Peers ◽  
Alan N. Bateson ◽  
Michael L. J. Ashford ◽  
...  
Keyword(s):  
Potassium Channel ◽  
Transcriptional Control ◽  
Chronic Hypoxia ◽  
Oxygen Sensitivity ◽  
Channel Activity ◽  
Potassium Channel Activity
Sign in / Sign up

Export Citation Format

Share Document