scholarly journals Active and inactive renin release from rabbit kidney cortex slices: effect of sodium concentration and of furosemide.

1982 ◽  
Vol 328 (1) ◽  
pp. 421-430 ◽  
Author(s):  
K A Munday ◽  
A R Noble ◽  
H K Richards
Keyword(s):  
Sodium Concentration ◽  
Renin Release ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Inactive Renin ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Isoprenaline-Induced Secretion of Active and Inactive Renin in Anaesthetized Rabbits and by Kidney Cortex Slices

1981 ◽  
Vol 61 (6) ◽  
pp. 679-684 ◽  
Author(s):  
H. K. Richards ◽  
A. R. Noble ◽  
K. A. Munday
Keyword(s):  
Time Course ◽  
Urine Volume ◽  
Renin Release ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Active Renin ◽  
Arterial Blood ◽  
Inactive Renin ◽  
Cortex Slices ◽  
Kidney Cortex Slices

1. The effect of the β-adrenoceptor agonist isoprenaline on the secretion of active and inactive renin was investigated in two preparations. 2. in ten urethane-anaesthetized rabbits isoprenaline, given as a renal artery infusion, had relatively minor effects on renal sodium excretion (increased) and systemic arterial blood pressure (decreased). Urine volume, potassium excretion, creatinine clearance and serum electrolytes were all unchanged. Plasma active and inactive renin both increased immediately and returned to basal values after ceasing the isoprenaline infusion. 3. No significant changes in either plasma renin activity or renal function were observed in a group of ten control animals. 4. The magnitude of the isoprenaline-induced changes in plasma active renin was similar to that in a previous study of frusemide diuresis, but the time course was quite different. Inactive renin disappeared from plasma during frusemide diuresis. 5. Renin release by rabbit kidney cortex slices was also studied. Isoprenaline, added to the incubation medium, caused a dose-related increase in active renin secretion, but inactive renin release remained unchanged. This is in marked constrast to a previous study where reducing [Na+] increased active renin and inhibited inactive renin output. 6. These data support our previous suggestion that activation of inactive renin is regulated by a sodium-sensitive intrarenal mechanism.

Effect of osmotic shocks on rabbit kidney cortex slices

1983 ◽  
Vol 244 (6) ◽  
pp. F696-F705
Author(s):  
R. Gilles ◽  
C. Duchene ◽  
I. Lambert
Keyword(s):  
Cell Volume ◽  
Volume Regulation ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Regulation Mechanism ◽  
Kidney Slices ◽  
Regulation Process ◽  
Maximum Cell ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Rabbit kidney cortex slices behave an osmometers when withstanding hyperosmotic or hyposmotic shocks of amplitude up to pi 1/pi 2 = 1.25. For hyposmotic shocks of amplitude larger than or equal to pi 1/pi 2 = 1.50, the maximum swelling achieved is less than what can be expected on the basis of the van't Hoff relation, thereby indicating that a volume regulation process is taking place. Volume regulation in kidney slices can be dissociated into two distinct phases. The first one, of swelling limitation, is very rapid and keeps maximum cell volume at values lower than expected when the tissue is considered as an osmometer. This phase is followed by a slow volume readjustment process during which volume progressively decreases towards control values. The major intracellular osmotic effector loss during both swelling limitation and volume readjustment is Na+. The overall volume regulation process is insensitive to furosemide, vanadate, and bumetanide. Swelling limitation is blocked by addition of ouabain. Contrary to what has been believed previously, there is, however, no need to implicate control of the activity of a ouabain-sensitive, Na+/K+ pump in the Na-dependent volume regulation mechanism.

RENIN SECRETION IN VITRO FROM RAT KIDNEY CORTEX SLICES

1969 ◽  
Vol 60 (3) ◽  
pp. 550-554 ◽  
Author(s):  
Lj. Božović ◽  
S. Efendić
Keyword(s):  
Rat Kidney ◽  
Renin Secretion ◽  
Renin Release ◽  
Kidney Cortex ◽  
Rat Kidney Cortex ◽  
Active Mechanism ◽  
Cortex Slices ◽  
Kidney Cortex Slices

ABSTRACT A method for in vitro studies of renin release is described. Kidney cortex slices taken from control rats and rats stimulated to release renin were incubated with and without glucose. Renin release from the slices to a large extent was glucose-dependent. This result supports the hypothesis of an active mechanism of renin secretion.

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