scholarly journals Climbing fibre induced depression of both mossy fibre responsiveness and glutamate sensitivity of cerebellar Purkinje cells

1982 ◽  
Vol 324 (1) ◽  
pp. 113-134 ◽  
Author(s):  
Masao Ito ◽  
Masaki Sakurai ◽  
Pavich Tongroach
Keyword(s):  
Purkinje Cells ◽  
Mossy Fibre ◽  
Climbing Fibre ◽  
Cerebellar Purkinje Cells

Incomplete regression of multiple climbing fibre innervation of cerebellar Purkinje cells in mGluR1 mutant mice

Neuroreport ◽  
1997 ◽  
Vol 8 (2) ◽  
pp. 571-574 ◽  
Author(s):  
Carole Levenes ◽  
Hervé Daniel ◽  
Danielle Jaillard ◽  
François Conquet ◽  
François Crépel
Keyword(s):  
Purkinje Cells ◽  
Mutant Mice ◽  
Climbing Fibre ◽  
Cerebellar Purkinje Cells

scholarly journals Long-term climbing fibre activity induces transcription of microRNAs in cerebellar Purkinje cells

2014 ◽  
Vol 369 (1652) ◽  
pp. 20130508 ◽  
Author(s):  
Neal H. Barmack ◽  
Zuyuan Qian ◽  
Vadim Yakhnitsa
Keyword(s):  
Purkinje Cells ◽  
Fold Increase ◽  
Long Term Potentiation ◽  
Serine Phosphorylation ◽  
Behavioural Plasticity ◽  
Synaptic Membrane ◽  
Climbing Fibre ◽  
Mrna Targets ◽  
Cerebellar Purkinje Cells

Synaptic activation of central neurons is often evoked by electrical stimulation leading to post-tetanic potentiation, long-term potentiation or long-term depression. Even a brief electrical tetanus can induce changes in as many as 100 proteins. Since climbing fibre activity is often associated with cerebellar behavioural plasticity, we used horizontal optokinetic stimulation (HOKS) to naturally increase synaptic input to floccular Purkinje cells in mice for hours, not minutes, and investigated how this activity influenced the transcription of microRNAs, small non-coding nucleotides that reduce transcripts of multiple, complementary mRNAs. A single microRNA can reduce the translation of as many as 30 proteins. HOKS evoked increases in 12 microRNA transcripts in floccular Purkinje cells. One of these microRNAs, miR335, increased 18-fold after 24 h of HOKS. After HOKS stopped, miR335 transcripts decayed with a time constant of approximately 2.5 h. HOKS evoked a 28-fold increase in pri-miR335 transcripts compared with an 18-fold increase in mature miR335 transcripts, confirming that climbing fibre-evoked increases in miR335 could be attributed to increases in transcription. We used three screens to identify potential mRNA targets for miR335 transcripts: (i) nucleotide complementarity, (ii) detection of increased mRNAs following microinjection of miR335 inhibitors into the cerebellum, and (iii) detection of decreased mRNAs following HOKS. Two genes, calbindin and 14-3-3-θ, passed these screens. Transfection of N2a cells with miR335 inhibitors or precursors inversely regulated 14-3-3-θ transcripts. Immunoprecipitation of 14-3-3-θ co-immunoprecipitated PKC-γ and GABA A γ 2 . Knockdown of either 14-3-3-θ or PKC-γ decreased the serine phosphorylation of GABA A γ 2 , suggesting that 14-3-3-θ and PKC-γ under the control of miR335 homeostatically regulate the phosphorylation and insertion of GABA A γ 2 into the Purkinje cell post-synaptic membrane.

The effects of ethanol on cerebellar Purkinje cells in naive and alcohol-dependent rats

1980 ◽  
Vol 58 (4) ◽  
pp. 429-432 ◽  
Author(s):  
J. G. Sinclair ◽  
G. F. Lo ◽  
A. F. Tien
Keyword(s):  
Purkinje Cells ◽  
Acute Tolerance ◽  
Climbing Fibre ◽  
Anaesthetized Rats ◽  
Cerebellar Purkinje Cells ◽  
Alcohol Dependent

In urethane-anaesthetized rats, ethanol (1.5 g/kg, i.v.) increased the rate of discharge of cerebellar Purkinje cells, increased their regularity of discharge, and reduced or eliminated the climbing fibre evoked bursts. Most of the neurones responding in this manner exhibited an acute tolerance to the drug. Another group of rats was made alcohol dependent by feeding them for 2 weeks on a diet containing ethanol (12.5–16.5 g/kg per day). Ethanol produced similar but reduced effects on Purkinje cells of these animals.

scholarly journals Ethanol effects on the olivocerebellar system

1982 ◽  
Vol 60 (5) ◽  
pp. 610-614 ◽  
Author(s):  
J. G. Sinclair ◽  
G. F. Lo ◽  
D. P. Harris
Keyword(s):  
Purkinje Cell ◽  
Purkinje Cells ◽  
Inferior Olive ◽  
Climbing Fibre ◽  
Complex Spike ◽  
Anaesthetized Rats ◽  
Cerebellar Purkinje Cells ◽  
Cell Synapse

Ethanol (1.5 g/kg i.v.) was found to decrease spontaneous complex spike (CS) activity in cerebellar Purkinje cells in urethane anaesthetized rats while not changing the threshold required to evoke a CS by juxtafastigial stimulation. Thus ethanol does not decrease CS activity by an action at the climbing fibre – Purkinje cell synapse. Tremor induced by harmaline (5 mg/kg i.v.) in unanaesthetized animals was markedly antagonized by ethanol (0.5–2.0 g/kg i.v.) in all animals tested. However, in nine urethane-anaesthetized animals, ethanol markedly reversed the effects of harmaline on Purkinje cells in only two cases and partially reversed the effects in another four cells. Thus, the depressant effects of ethanol on the inferior olive is not totally responsible for the blockade of the harmaline tremor but would account for the decrease in spontaneous CS activity.

Ultrastorcture of Cerebellar Purkinje Cells in Rats Subjected To Partial Global Cerebral Ischemia

1985 ◽  
Vol 43 ◽  
pp. 674-675
Author(s):  
R.V.W. Dimlich ◽  
M.H. Biros
Keyword(s):  
Cerebral Ischemia ◽  
Purkinje Cells ◽  
Cell Types ◽  
Microscopic Level ◽  
Global Cerebral Ischemia ◽  
Light Microscopic Level ◽  
Cerebellar Damage ◽  
Cerebellar Injury ◽  
Cerebellar Purkinje Cells ◽  
Cell Changes

In severe cerebral ischemia, Purkinje cells of the cerebellum are one of the cell types most vulnerable to anoxic damage. In the partial (forebrain) global ischemic (PGI) model of the rat, Paljärvi noted at the light microscopic level that cerebellar damage is inconsistant and when present, milder than in the telencephalon, diencephalon and rostral brain stem. Cerebellar injury was observed in 3 of 4 PGI rats following 5 minutes of reperfusion but in none of the rats after 90 min of reperfusion. To evaluate a time between these two extremes (5 and 90 min), the present investigation used the PGI model to study the effects of ischemia on the ultrastructure of cerebellar Purkinje cells in rats that were sacrificed after 30 min of reperfusion. This time also was chosen because lactic acid that is thought to contribute to ischemic cell changes in PGI is at a maximum after 30 min of reperfusion.

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